聚乳酸接枝丙烯酸纳米孔纤维膜制备及其细胞相容性研究
发布时间：2018-11-23 18:51
【摘要】：以V(二氯甲烷)/V(N,N-二甲基甲酰胺)=4∶1为溶剂,通过电纺制备直径为(650±60)nm,孔径为96 nm×72 nm聚乳酸(PLLA)纳米孔纤维膜。利用氧等离子体处理将亲水性单体丙烯酸(AA)接枝到纤维表面制备聚乳酸接枝丙烯酸(PLLAg-PAA)纳米孔纤维膜。与PLLA纳米孔纤维膜相比,PLLA-g-PAA纳米孔纤维膜的水接触角从(119.4±1.2)°降低到(42.3±0.6)°,拉伸强度、杨氏模量和断裂伸长率略有降低。将牙髓干细胞(DPSCs)在纤维膜支架上培养,细胞生长密度顺序为PLLA-g-PAA纳米孔纤维膜PLLA纳米孔纤维膜PLLA纤维膜。由于PLLA-g-PAA纳米孔纤维膜表面粗糙,比表面积大,孔隙率高和表面亲水性好,更有利于细胞的粘附、迁移、分化和繁殖。PLLA-g-PAA纳米孔纤维膜有望成为优良的组织工程支架材料。
[Abstract]:Using V (dichloromethane) / V (NN- dimethylformamide) = 4:1 as solvent, the membrane with a diameter of (650 卤60) nm, with a pore diameter of 96 nm 脳 72 nm Poly (lactic acid) (PLLA) nanoporous fiber was prepared by electrospinning. Poly (lactic acid) grafted acrylic acid (PLLAg-PAA) nano-porous fiber membrane was prepared by oxygen plasma treatment by grafting hydrophilic monomer acrylic acid (AA) onto the fiber surface. Compared with PLLA nano-porous fiber membrane, the water contact angle of PLLA-g-PAA nano-porous fiber membrane decreased from (119.4 卤1.2) 掳to (42.3 卤0.6) 掳, and the tensile strength, Young's modulus and elongation at break decreased slightly. Dental pulp stem cell (DPSCs) was cultured on fiber membrane scaffold. The order of cell growth density was PLLA-g-PAA nano-pore fiber membrane PLLA fiber membrane. Because of the rough surface, large specific surface area, high porosity and good surface hydrophilicity of PLLA-g-PAA nanoporous fiber membrane, it is more favorable for cell adhesion and migration. Differentiation and reproduction. PLLA-g-PAA nanoporous fiber membrane is expected to be an excellent scaffold material for tissue engineering.
【作者单位】： 武夷学院生态与资源工程学院福建省生态产业绿色技术重点实验室;福建师范大学材料科学与工程学院;
【基金】：国家自然科学基金(51406141) 福建省教育厅(JK2014052,JZ160333) 南平市科技局(2011DJ10)
【分类号】：R318.08;TQ342.8
本文编号：2352414
[Abstract]:Using V (dichloromethane) / V (NN- dimethylformamide) = 4:1 as solvent, the membrane with a diameter of (650 卤60) nm, with a pore diameter of 96 nm 脳 72 nm Poly (lactic acid) (PLLA) nanoporous fiber was prepared by electrospinning. Poly (lactic acid) grafted acrylic acid (PLLAg-PAA) nano-porous fiber membrane was prepared by oxygen plasma treatment by grafting hydrophilic monomer acrylic acid (AA) onto the fiber surface. Compared with PLLA nano-porous fiber membrane, the water contact angle of PLLA-g-PAA nano-porous fiber membrane decreased from (119.4 卤1.2) 掳to (42.3 卤0.6) 掳, and the tensile strength, Young's modulus and elongation at break decreased slightly. Dental pulp stem cell (DPSCs) was cultured on fiber membrane scaffold. The order of cell growth density was PLLA-g-PAA nano-pore fiber membrane PLLA fiber membrane. Because of the rough surface, large specific surface area, high porosity and good surface hydrophilicity of PLLA-g-PAA nanoporous fiber membrane, it is more favorable for cell adhesion and migration. Differentiation and reproduction. PLLA-g-PAA nanoporous fiber membrane is expected to be an excellent scaffold material for tissue engineering.
【作者单位】： 武夷学院生态与资源工程学院福建省生态产业绿色技术重点实验室;福建师范大学材料科学与工程学院;
【基金】：国家自然科学基金(51406141) 福建省教育厅(JK2014052,JZ160333) 南平市科技局(2011DJ10)
【分类号】：R318.08;TQ342.8
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