类人胶原蛋白-微孔淀粉的制备及生物学评价
发布时间：2018-11-23 19:58
【摘要】：文中以微孔淀粉为主体,研究一种新型复合止血材料的制备及生物学评价。此复合材料由一定比例的α-淀粉酶与糖化酶复合后处理淀粉以形成微孔结构,然后复合类人胶原蛋白,再以戊二醛为交联剂进行交联;用SD大鼠作肝脏出血模型进行止血效果对比试验。通过正交试验确定微孔淀粉最优制备工艺条件如下:α-淀粉酶:糖化酶=1∶3,T/℃=50,t/h=20,pH=4.8;用0.2%(V/V)戊二醛乙醇溶液交联的类人胶原蛋白-微孔淀粉止血材料,在相同的止血时间下,该复合材料平均吸水率为3 580%,对照组(分别是微孔淀粉和类人胶原蛋白海绵)的平均吸水率为3 160%和3 280%。而在SD大鼠肝脏实验中,复合材料t/s=94±13,而外购的止血剂t/s=121±15。类人胶原蛋白-微孔淀粉复合材料止血的止血原理从3个方面展开:①微孔淀粉吸收水分并将血液中的有形物质聚集到表面;②加速内源性凝血因子的激活使其形成血栓;③在类人胶原蛋白的牵引下,淀粉颗粒聚成团大幅度增加了比表面积,使止血效果更优秀;对照组止血机理只包含了该复合材料的前两个方面。结果显示,复合材料的止血效果要优于普通微孔淀粉制成的止血剂,证明该复合止血材料具有良好的止血性能。
[Abstract]:In this paper, the preparation and biological evaluation of a new compound hemostatic material with microporous starch as the main body were studied. The composite was treated with a certain proportion of 伪 -amylase and Glucoamylase to form micropore structure, and then compound human collagen-like protein, then crosslinked with glutaraldehyde as crosslinking agent. The hemostatic effect of SD rats was compared with the model of hepatic hemorrhage. The optimum preparation conditions of microporous starch were determined by orthogonal test as follows: 伪 -amylase: Glucoamylase = 1: 3T / 鈩，
本文编号：2352547
[Abstract]:In this paper, the preparation and biological evaluation of a new compound hemostatic material with microporous starch as the main body were studied. The composite was treated with a certain proportion of 伪 -amylase and Glucoamylase to form micropore structure, and then compound human collagen-like protein, then crosslinked with glutaraldehyde as crosslinking agent. The hemostatic effect of SD rats was compared with the model of hepatic hemorrhage. The optimum preparation conditions of microporous starch were determined by orthogonal test as follows: 伪 -amylase: Glucoamylase = 1: 3T / 鈩，
本文编号：2352547
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