矿化柞蚕丝胶膜表面粗糙度的调控及其对骨髓间充质干细胞生长行为的影响

发布时间：2018-11-25 15:51

【摘要】：生物材料表面的粗糙度是影响细胞行为的重要因素之一。为了调控丝蛋白生物材料表面的粗糙度,并评价材料表面粗糙度对细胞生长行为的影响,首先,通过湿化学共沉淀法,以柞蚕丝胶(AS)溶液为模板,诱导了羟基磷灰石(HAp)晶体成核,进而调控了AS膜表面的粗糙度。然后,采用SEM、粗糙仪、FTIR及EDX等对HAp/AS复合膜表面形貌、粗糙度及成分进行了表征。最后,通过SEM和CellTiter 96?AQueous单溶液细胞增殖检测试剂盒(MTS)检测了骨髓间充质干细胞(BMSCs)在HAp/AS复合膜表面的形貌及增殖率。结果表明:纯AS膜的表面粗糙度为0.15μm,矿化1、8及24h后,表面粗糙度分别为0.38、0.46和1.20μm;矿化24h后,在HAp/AS复合膜表面可观察到直径为30~80nm的球状复合物,生成的矿化物为HAp;HAp/AS复合膜具有良好的细胞相容性,表面粗糙度为1.20μm的复合膜能够显著促进BMSCs的增殖,粗糙度对BMSCs在HAp/AS复合膜表面的粘附和形貌有着重要的影响。因此,可通过矿化的方法在生物大分子表面诱导HAp晶体的成核与生长,从而调控材料的表面粗糙度,研究材料界面上的细胞行为。
[Abstract]:Surface roughness of biomaterials is one of the important factors affecting cell behavior. In order to regulate the surface roughness of silk protein biomaterials and evaluate the effect of surface roughness on cell growth behavior, firstly, the solution of tussah sericin (AS) was used as template by wet chemical coprecipitation. The nucleation of hydroxyapatite (HAp) crystal was induced and the surface roughness of AS film was regulated. Then, the surface morphology, roughness and composition of HAp/AS composite film were characterized by SEM, roughness, FTIR and EDX. Finally, the morphology and proliferation rate of bone marrow mesenchymal stem cells (BMSCs) on the surface of HAp/AS composite membrane were detected by SEM and CellTiter 96?AQueous single cell proliferation assay kit (MTS). The results show that the surface roughness of pure AS films is 0. 15 渭 m, and the surface roughness of pure AS films is 0. 38 渭 m and 1. 20 渭 m after mineralization for 1 ~ 8 and 24 h, respectively. After 24 hours of mineralization, spherical complexes with diameter of 30~80nm were observed on the surface of HAp/AS composite membrane. The mineralized compound was HAp;. HAp/AS composite membrane has good cell compatibility. The surface roughness of 1.20 渭 m composite membrane can significantly promote the proliferation of BMSCs. Roughness has an important effect on the adhesion and morphology of BMSCs on the surface of HAp/AS composite membrane. Therefore, the formation and growth of HAp crystals can be induced by mineralization on the surface of biomolecules, thus controlling the surface roughness of the materials and studying the cell behavior at the interface of the materials.
【作者单位】：浙江大学动物科学学院;浙江大学医学院;
【基金】：国家“863”计划(2013AA102507) 国家自然科学基金(21172194) 浙江省自然科学基金重点项目(LZ12C17001) 浙江省蚕桑产业科技创新团队项目(2011R50028)
【分类号】：R318.08

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