核素切伦科夫光学显像基础研究
发布时间：2018-12-10 22:01
【摘要】：目的 研究不同核素的切伦科夫光学显像的光学信号在不同组织中的穿透性差异及空间分辨率变化。并且设计一种稀土纳米颗粒对切伦科夫光进行转换以增强其信号强度和穿透性。 方法 将活度为0，3，6，12，25，50uCi的131I和18F-FDG分别加入24孔板中，进行切伦科夫光学显像，进行切伦科夫光学信号与放射性活度的相关性分析。制作放射性浓度为20，50，100，200μCi/μl，长度为100mm，，直径为1mm的线状131I放射源，间隔1cm平行放置，分别覆盖不同厚度的脂肪、肝、肺和肌肉组织片，进行光学显像；观察不同活度的131I对不同组织的光信号穿透性差异。制作放射性浓度为100uCi/μL，长度为100mm，直径为1mm的线状131I和18F放射源，间隔2cm平行放置；上方覆盖不同厚度的脂肪、肝、肺和肌肉组织薄片进行切伦科夫光学显像，研究切伦科夫光学显像空间分辨率的变化。然后利用水热法合成Yb3+-和Er3+-掺杂的NaYF4稀土纳米颗粒。利用扫描电镜和X射线衍射对其进行表征并利用光谱仪测定其吸收和发射光谱。将其溶于水中加入发射性核素，利用光学显像测定其对切伦科夫光信号的增强作用，同时利用仿体模型测定加入稀土纳米颗粒后核素切伦科夫光的穿透性变化。最红利用荷假瘤小鼠进行正电子发射断层显像和光学显像验证这一增强效果。 结果 切伦科夫光信号强度随放射性活度的增加呈线性增加(r2=0.97，P=0.0001和r2=0.96，P=0.0001)。同时切伦科夫光信号的强度随光源的深度增加而下降。其中以脂肪组织的光透过性最好。另外，切伦科夫光学显像的空间分辨率随放射性活度的降低和组织深度的增加而下降；同时对不同组织的空间分辨率各异，其中以脂肪组织中成像的空间分辨率最好。之后的增强实验显示切伦科夫辐射可以在520nm和980nm波段激发稀土纳米颗粒在660nm波段发光。加入稀土纳米颗粒后，切伦科夫光强度提升约2倍而组织穿透性从5mm明显提高至15mm。并且信号增强的强度与放射性核素活度(R2=0.996)及稀土纳米颗粒的质量(R2=0.994)有关。动物实验则验证了这一效应的可行性。 结论 切伦科夫光学显像的光穿透性及空间分辨率受核素类型、放射性活度、组织类型及组织深度等因素影响较为明显。其光信号穿透性差，而分辨率较高。在加入稀土纳米颗粒后，切伦科夫光信号强度和穿透性都明显提升，为进一步的应用奠定了基础。
[Abstract]:Aim to study the difference of optical signal penetration and spatial resolution in different tissues of Cerenkov optical imaging with different nuclides. A rare earth nanoparticles were designed to convert the Cerenkov light to enhance its signal intensity and penetration. Methods 131I and 18F-FDG were added to the 24-hole plate respectively, and the correlation between the Cerenkov optical signal and the radioactivity was analyzed. A linear 131I radioactive source with a diameter of 100mm and a diameter of 1mm 渭 Ci/ 渭 l was prepared. The spacer 1cm was placed parallel to each other and covered with different thickness of fat, liver, lung and muscle tissue, respectively, for optical imaging. The optical signal penetration of 131I with different activity to different tissues was observed. 100uCi/ 渭 L, 100mm long, 131I and 18F radioactive sources with diameter of 1mm were prepared, and the 2cm was spaced parallel to each other. The different thickness of fat, liver, lung and muscle tissue were covered with Cerenkov optical imaging to study the spatial resolution of Cerenkov optical imaging. Then Yb3-and Er3-doped NaYF4 rare earth nanoparticles were synthesized by hydrothermal method. The absorption and emission spectra were characterized by scanning electron microscope (SEM) and X-ray diffraction (XRD). The enhancement of Cerenkov light signal was measured by optical imaging, and the penetrability of Cerenkov light with rare earth nanoparticles was measured by the imitating body model. Positron emission tomography (PET) and optical imaging were performed in mice bearing pseudotumor. Results the intensity of Cerenkov light signal increased linearly with the increase of radioactivity (r _ (2) O ~ (97) P ~ (0. 0001) and r ~ (2 +) ~ (0.96) P ~ (2 +) P ~ (0.0001). At the same time, the intensity of the Cherenkov light signal decreases with the increase of the depth of the light source. Among them, adipose tissue has the best light transmittance. In addition, the spatial resolution of Cerenkov optical imaging decreases with the decrease of radioactivity and the increase of tissue depth, and the spatial resolution of different tissues is different, and the spatial resolution of imaging in adipose tissue is the best. The subsequent enhancement experiments show that Cerenkov radiation can excite rare earth nanoparticles in the 520nm and 980nm bands to emit light in the 660nm band. With the addition of rare earth nanoparticles, the light intensity of Cerenkov was increased by about 2 times, while the penetration of the tissue was increased from 5mm to 15mm. The intensity of signal enhancement is related to the activity of radionuclide (R2N) 0.996 and the mass of rare earth nanoparticles (R2O0.994). Animal experiments verify the feasibility of this effect. Conclusion the optical penetrability and spatial resolution of Cerenkov optical imaging are obviously influenced by the type of radionuclide, the activity of radionuclide, the type of tissue and the depth of tissue. The optical signal has poor penetration and high resolution. After the addition of rare earth nanoparticles, the intensity and penetration of Cerenkov light signal were improved obviously, which laid a foundation for further application.
【学位授予单位】：第四军医大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R310
[Abstract]:Aim to study the difference of optical signal penetration and spatial resolution in different tissues of Cerenkov optical imaging with different nuclides. A rare earth nanoparticles were designed to convert the Cerenkov light to enhance its signal intensity and penetration. Methods 131I and 18F-FDG were added to the 24-hole plate respectively, and the correlation between the Cerenkov optical signal and the radioactivity was analyzed. A linear 131I radioactive source with a diameter of 100mm and a diameter of 1mm 渭 Ci/ 渭 l was prepared. The spacer 1cm was placed parallel to each other and covered with different thickness of fat, liver, lung and muscle tissue, respectively, for optical imaging. The optical signal penetration of 131I with different activity to different tissues was observed. 100uCi/ 渭 L, 100mm long, 131I and 18F radioactive sources with diameter of 1mm were prepared, and the 2cm was spaced parallel to each other. The different thickness of fat, liver, lung and muscle tissue were covered with Cerenkov optical imaging to study the spatial resolution of Cerenkov optical imaging. Then Yb3-and Er3-doped NaYF4 rare earth nanoparticles were synthesized by hydrothermal method. The absorption and emission spectra were characterized by scanning electron microscope (SEM) and X-ray diffraction (XRD). The enhancement of Cerenkov light signal was measured by optical imaging, and the penetrability of Cerenkov light with rare earth nanoparticles was measured by the imitating body model. Positron emission tomography (PET) and optical imaging were performed in mice bearing pseudotumor. Results the intensity of Cerenkov light signal increased linearly with the increase of radioactivity (r _ (2) O ~ (97) P ~ (0. 0001) and r ~ (2 +) ~ (0.96) P ~ (2 +) P ~ (0.0001). At the same time, the intensity of the Cherenkov light signal decreases with the increase of the depth of the light source. Among them, adipose tissue has the best light transmittance. In addition, the spatial resolution of Cerenkov optical imaging decreases with the decrease of radioactivity and the increase of tissue depth, and the spatial resolution of different tissues is different, and the spatial resolution of imaging in adipose tissue is the best. The subsequent enhancement experiments show that Cerenkov radiation can excite rare earth nanoparticles in the 520nm and 980nm bands to emit light in the 660nm band. With the addition of rare earth nanoparticles, the light intensity of Cerenkov was increased by about 2 times, while the penetration of the tissue was increased from 5mm to 15mm. The intensity of signal enhancement is related to the activity of radionuclide (R2N) 0.996 and the mass of rare earth nanoparticles (R2O0.994). Animal experiments verify the feasibility of this effect. Conclusion the optical penetrability and spatial resolution of Cerenkov optical imaging are obviously influenced by the type of radionuclide, the activity of radionuclide, the type of tissue and the depth of tissue. The optical signal has poor penetration and high resolution. After the addition of rare earth nanoparticles, the intensity and penetration of Cerenkov light signal were improved obviously, which laid a foundation for further application.
【学位授予单位】：第四军医大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R310
【共引文献】
相关期刊论文 前10条
1 康飞;杨卫东;汪静;;对切伦科夫光学成像应用前景的思考[J];医学争鸣;2013年06期
2 张庆彬;孔祥贵;王新;程成;;NaYF_4:Yb~(3+),Er~(3+)上转换发光纳米晶在三元混合溶剂体系中的配体交换修饰及发光性质[J];高等学校化学学报;2014年02期
3 吕光磊;刘克印;孟路燕;易涛;;二芳乙烯荧光开关材料及其应用进展[J];影像科学与光化学;2014年01期
4 杨卫东;汪静;;核素切伦科夫显像研究进展[J];功能与分子医学影像学(电子版);2013年03期
5 李向丽;谭贵良;张娜;刘W
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