新型牙髓治疗材料iRoot BP Plus对人牙龈成纤维细胞的细胞毒性
发布时间:2018-12-14 13:35
【摘要】:目的:比较新型牙髓治疗材料i Root BP Plus与矿物三氧化物凝聚体(mineral trioxide aggregate,MTA)对人牙龈成纤维细胞的细胞毒性。方法:采用四甲基偶氮唑盐(methyl-thiazol-tetrazolium,MTT)法测定上述2种材料不同浸提时间(1、3、7 d)的浸提液原液及不同浓度稀(1:2、1:5)释液对体外培养的人牙龈成纤维细胞增殖的影响。细胞增殖率以x±s表示,采用SPSS20.0软件包对数据进行析因设计的方差分析。结果:i Root BP Plus和MTA的浸提液原液及不同浓度稀释液作用后的细胞增殖率界于77.31%~113.82%,细胞毒性分级为0或1级,评价结果为无细胞毒性。不同时间点、不同稀释度下,2种材料对体外培养的人牙龈成纤维细胞增殖率的影响无显著差异(F浓度*时间*材料=1.393,P=0.256)。结论 :i Root BP Plus与MTA均对体外培养的人牙龈成纤维细胞无细胞毒性。
[Abstract]:Aim: to compare the cytotoxicity of i Root BP Plus and (mineral trioxide aggregate,MTA on human gingival fibroblasts. Methods: tetramethylazolium salt (methyl-thiazol-tetrazolium,MTT) was used to determine the extraction time of the two materials. The effects of 1: 2 and 1: 5 release solution on the proliferation of human gingival fibroblasts in vitro. The cell proliferation rate was expressed as x 卤s, and SPSS20.0 software package was used to analyze the variance of the factorial design. Results the cell proliferation rate of: i Root BP Plus and MTA was 77.31 and 113.82, and the cytotoxicity grade was 0 or 1. The results showed that there was no cytotoxicity. There was no significant difference in the proliferation rate of human gingival fibroblasts between the two materials at different time points and different dilutions (F concentration * time * material = 1.393). Conclusion both: i Root BP Plus and MTA have no cytotoxicity to cultured human gingival fibroblasts.
【作者单位】: 中国医科大学口腔医学院儿童口腔科;辽宁省口腔医学研究所儿童口腔医学研究室;
【基金】:辽宁省科学技术计划项目(2012225015) 沈阳市科学技术计划项目(F12-277-1-65)~~
【分类号】:R783.1
[Abstract]:Aim: to compare the cytotoxicity of i Root BP Plus and (mineral trioxide aggregate,MTA on human gingival fibroblasts. Methods: tetramethylazolium salt (methyl-thiazol-tetrazolium,MTT) was used to determine the extraction time of the two materials. The effects of 1: 2 and 1: 5 release solution on the proliferation of human gingival fibroblasts in vitro. The cell proliferation rate was expressed as x 卤s, and SPSS20.0 software package was used to analyze the variance of the factorial design. Results the cell proliferation rate of: i Root BP Plus and MTA was 77.31 and 113.82, and the cytotoxicity grade was 0 or 1. The results showed that there was no cytotoxicity. There was no significant difference in the proliferation rate of human gingival fibroblasts between the two materials at different time points and different dilutions (F concentration * time * material = 1.393). Conclusion both: i Root BP Plus and MTA have no cytotoxicity to cultured human gingival fibroblasts.
【作者单位】: 中国医科大学口腔医学院儿童口腔科;辽宁省口腔医学研究所儿童口腔医学研究室;
【基金】:辽宁省科学技术计划项目(2012225015) 沈阳市科学技术计划项目(F12-277-1-65)~~
【分类号】:R783.1
【共引文献】
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1 李峥;杜斌;苗群爱;;牙髓病治疗领域生物材料的研究进展[J];中国美容医学;2014年11期
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