选择性激光烧结法构建纳米羟基磷灰石与聚己内酯复合材料人工骨支架
发布时间：2019-01-27 10:27
【摘要】：目的用选择性激光烧结(selective laser sintering,SLS)技术构建纳米羟基磷灰石(nano-hydroxyapatite,Nano-HA)/聚己内酯(poly-ε-caprolactone,PCL)人工骨支架并探索其力学特性、生物相容性和生物活性。方法配制NanoHA质量分数分别为5%、10%、15%的Nano-HA/PCL混合材料粉末,采用SLS技术制备出PCL与Nano-HA/PCL(5%、10%、15%)人工骨支架,并测定其孔隙率及抗压强度。将分离培养的兔骨髓间充质干细胞(MSCs)接种至上述2种支架中,观察细胞黏附及增殖情况,并通过碱性磷酸酶(ALP)表达测定、茜素红法钙结节染色比较其成骨分化情况。结果各组支架均具有良好的力学强度和细胞黏附性,且未见明显的细胞毒性;接种后第1天各组的ALP表达差异无统计学意义,但随着培养时间的延长,Nano-HA/PCL组的ALP表达逐渐高于PCL组与空白对照组(P0.05),且Nano-HA比例越高,ALP的表达也越强(P0.05);Nano-HA/PCL组茜素红染色的阳性强度高于PCL组与空白对照组,且随着Nano-HA含量增高,其钙结节数量增多,染色的阳性强度也随之增加。结论本研究通过SLS技术所构建出的Nano-HA/PCL人工骨支架具有良好的生物力学强度、生物相容性及骨诱导性,有望成为一种新型骨修复替代材料。
[Abstract]:Objective to construct nano-hydroxyapatite (nano-hydroxyapatite,Nano-HA) / polycaprolactone (poly- 蔚-caprolactone,PCL) artificial bone scaffold by selective laser sintering (selective laser sintering,SLS) and explore its mechanical properties, biocompatibility and bioactivity. Methods the PCL and Nano-HA/PCL artificial bone scaffolds were prepared by SLS technique, and the porosity and compressive strength of the scaffolds were measured. Cultured rabbit bone marrow mesenchymal stem cells (MSCs) were inoculated into the above two scaffolds to observe the cell adhesion and proliferation. The osteogenic differentiation of rabbit bone marrow mesenchymal stem cells was determined by alkaline phosphatase (ALP) expression and the osteogenic differentiation was compared by alizarin red calcium nodule staining. Results the scaffolds had good mechanical strength and cell adhesion, and there was no obvious cytotoxicity. On the first day after inoculation, there was no significant difference in ALP expression in all groups, but with the extension of culture time, the ALP expression in Nano-HA/PCL group was gradually higher than that in PCL group and blank control group (P0.05), and the ratio of Nano-HA was higher than that in control group. The expression of ALP was stronger (P0.05). The positive intensity of alizarin red staining in Nano-HA/PCL group was higher than that in PCL group and blank control group. With the increase of Nano-HA content, the number of calcium nodules increased and the positive intensity of staining increased. Conclusion the Nano-HA/PCL artificial bone scaffold constructed by SLS technique has good biomechanical strength, biocompatibility and bone induction, and it is expected to be a new substitute for bone repair.
【作者单位】： 第二军医大学长海医院创伤骨科;解放军309医院骨科;解放军94826部队野战医院;兰州军区总医院骨科;解放军71677部队卫生队;
【基金】：国家杰出青年科学基金(81201442)~~
【分类号】：R318.08
本文编号：2416171
[Abstract]:Objective to construct nano-hydroxyapatite (nano-hydroxyapatite,Nano-HA) / polycaprolactone (poly- 蔚-caprolactone,PCL) artificial bone scaffold by selective laser sintering (selective laser sintering,SLS) and explore its mechanical properties, biocompatibility and bioactivity. Methods the PCL and Nano-HA/PCL artificial bone scaffolds were prepared by SLS technique, and the porosity and compressive strength of the scaffolds were measured. Cultured rabbit bone marrow mesenchymal stem cells (MSCs) were inoculated into the above two scaffolds to observe the cell adhesion and proliferation. The osteogenic differentiation of rabbit bone marrow mesenchymal stem cells was determined by alkaline phosphatase (ALP) expression and the osteogenic differentiation was compared by alizarin red calcium nodule staining. Results the scaffolds had good mechanical strength and cell adhesion, and there was no obvious cytotoxicity. On the first day after inoculation, there was no significant difference in ALP expression in all groups, but with the extension of culture time, the ALP expression in Nano-HA/PCL group was gradually higher than that in PCL group and blank control group (P0.05), and the ratio of Nano-HA was higher than that in control group. The expression of ALP was stronger (P0.05). The positive intensity of alizarin red staining in Nano-HA/PCL group was higher than that in PCL group and blank control group. With the increase of Nano-HA content, the number of calcium nodules increased and the positive intensity of staining increased. Conclusion the Nano-HA/PCL artificial bone scaffold constructed by SLS technique has good biomechanical strength, biocompatibility and bone induction, and it is expected to be a new substitute for bone repair.
【作者单位】： 第二军医大学长海医院创伤骨科;解放军309医院骨科;解放军94826部队野战医院;兰州军区总医院骨科;解放军71677部队卫生队;
【基金】：国家杰出青年科学基金(81201442)~~
【分类号】：R318.08
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