新型丝素蛋白多孔支架复合兔髓核细胞体外构建组织工程化髓核的实验研究
发布时间：2019-02-16 05:50
【摘要】：[目的]观察以新型丝素蛋白多孔支架复合兔髓核细胞体外构建组织工程化髓核的可行性。[方法]分离培养兔髓核细胞,与丝素蛋白多孔支架在体外复合培养,建立组织工程化髓核模型,通过扫描电镜、HE染色、甲苯胺蓝染色、Ⅱ型胶原免疫组化以及酶联免疫吸附测定观察细胞在支架上1周和3周的生长及增殖情况。[结果]培养1周后,扫描电镜显示细胞呈球状均匀地贴附在支架内部。细胞-支架复合体HE染色可见支架内部有大量髓核细胞,甲苯胺兰染色阳性,Ⅱ型胶原免疫组化染色阳性。培养3周后,扫描电镜显示细胞成层黏附于支架表面,细胞重叠生长,分泌大量细胞外基质,HE染色可见支架内部有大量髓核细胞填满支架孔隙并分泌大量细胞外基质,甲苯胺兰染色阳性,Ⅱ型胶原免疫组化染色阳性。酶联免疫吸附测定:3周组蛋白多糖含量(52.4±4.5)ng/ml明显高于1周组(29.3±3.6)ng/ml,P0.05,3周组的II型胶原含量(24.3±1.8)ng/ml明显高于1周组(15.16±1.5)ng/ml,P0.05。[结论]新型丝素蛋白多孔支架复合兔髓核细胞体外培养生长良好,分泌大量类似髓核样细胞外基质,可以用于体外构建组织工程化髓核。
[Abstract]:[objective] to observe the feasibility of constructing tissue engineered nucleus pulposus with a new fibroin porous scaffold combined with rabbit nucleus pulposus cells in vitro. [methods] the rabbit nucleus pulposus cells were isolated and cultured in vitro. The model of tissue engineered nucleus pulposus was established by co-culture with silk fibroin porous scaffold. Scanning electron microscopy, HE staining and toluidine blue staining were used. The growth and proliferation of type 鈪，
本文编号：2424109
[Abstract]:[objective] to observe the feasibility of constructing tissue engineered nucleus pulposus with a new fibroin porous scaffold combined with rabbit nucleus pulposus cells in vitro. [methods] the rabbit nucleus pulposus cells were isolated and cultured in vitro. The model of tissue engineered nucleus pulposus was established by co-culture with silk fibroin porous scaffold. Scanning electron microscopy, HE staining and toluidine blue staining were used. The growth and proliferation of type 鈪，
本文编号：2424109
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