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宽带激光熔覆钙磷涂层中的稀土可控析出及其对破骨细胞活性的抑制作用

发布时间:2019-02-21 20:01
【摘要】:人工关节的骨溶解(磨屑病)是导致人工假体松动的主要原因。利用稀土离子抑制破骨细胞的活性将会有效地防治骨溶解的产生。本文利用激光熔覆技术对生物医用金属材料(Ti-6Al-4V)表面进行改性处理,制备出含有稀土的Ca-P生物陶瓷涂层,利用涂层在生理环境中析出的稀土离子来达到防治骨溶解的目的。利用金相显微镜(OM)、扫描电镜(SEM)、等离子质谱仪(ICP-AES)、X射线衍射仪(XRD)、显微硬度仪、MTT比色法、倒置荧光显微镜,TRAP染色法等检测手段分别对Ca-P生物陶瓷涂层结合界面、微观形貌、稀土离子析出规律、物相、显微硬度、生物活性以及稀土离子浓度对破骨细胞活性抑制作用的规律进行了研究,并获得了以下结论:(1)探究了不同La_2O_3掺杂量及不同钙磷比对激光熔覆Ca-P陶瓷涂层组织性能与生物活性的影响。结果表明:Ca-P陶瓷涂层主要包括CaTiO_3、HA、β-TCP、TiO_2等物相。涂层中Ca:P比值越高,平均硬度越大。La_2O_3掺杂量为0.6 wt.%,Ca:P=1.40的Ca-P陶瓷涂层具有最佳的组织性能与生物活性。(2)探究了Ca-P陶瓷涂层在模拟体液中的降解析出规律。结果表明:Ca-P陶瓷涂层在模拟体液中质量呈现动态变化的规律,是类骨磷灰石沉积与β-TCP降解共存的一种动态变化。涂层中的La~(3+)平均每天的析出浓度在9.5~12 PPB之间波动变化。并计算得到Ca-P涂层中La~(3+)析出浓度C与浸泡时间t之间的规律公式为:(?),单位(PPB/mm~2)。(3)探究了Ca-P陶瓷涂层中可控析出的稀土离子对破骨细胞活性的抑制作用。结果表明:当细胞诱导因子浓度RANKL为100 ng/mL,M-CSF为50 ng/mL时,诱导生成的阳性多核破骨细胞数量最多。设置3天,6天,9天三个时间点,从统计数据可以看出,与空白组相比,La~(3+)浓度为21 PPB时,诱导生成的破骨细胞数量显著增加,P0.05。其他浓度的La~(3+)对诱导生成的破骨细胞数量具有明显的抑制作用。综上可知当La_2O_3含量为0.6 wt.%,Ca:P=1.40的Ca-P陶瓷涂层不仅具有优异的组织性能及生物活性,并且具有抑制破骨细胞活性的作用时间可以长达16.5年左右,期望其可为临床实践上防治磨屑病提供一种新思路。
[Abstract]:Osteolysis of artificial joint (debris disease) is the main cause of artificial prosthesis loosening. The inhibition of osteoclast activity by rare earth ions will effectively prevent the formation of osteolysis. In this paper, the surface of biomedical metal material (Ti-6Al-4V) was modified by laser cladding, and the Ca-P bioceramics coating containing rare earth was prepared. The rare earth ions precipitated by the coating in physiological environment were used to prevent and cure osteolysis. Metallographic microscope (OM), scanning electron microscope (SEM), plasma mass spectrometer (ICP-AES), X ray diffractometer (XRD), microhardness instrument MTT colorimetric method inverted fluorescence microscope) was used. The interface of Ca-P bioceramics coating, micromorphology, precipitation regularity of rare earth ions, phase and microhardness of Ca-P bioceramics coating were examined by TRAP staining method. Biological activity and the inhibition of rare earth ion concentration on osteoclast activity were studied. The following conclusions were obtained: (1) the effects of different amounts of La_2O_3 doping and different Ca / P ratios on the microstructure, properties and biological activity of laser clad Ca-P ceramic coatings were investigated. The results show that the Ca-P ceramic coating mainly includes CaTiO_3,HA, 尾-TCP,TiO_2 phase. The higher the Ca:P ratio in the coating, the greater the average hardness. The doping amount of La_2O_3 is 0.6 wt.%,. The Ca-P ceramic coating of Ca:P=1.40 has the best microstructure, properties and biological activity. (2) the degradation and precipitation of Ca-P ceramic coating in simulated body fluid was investigated. The results show that the quality of Ca-P ceramic coating changes dynamically in simulated body fluid, which is a dynamic change of the coexistence of bone-like apatite deposition and 尾-TCP degradation. The average daily precipitation concentration of La~ (3) in the coating fluctuates from 9.5 to 12 PPB. The formula between the concentration C of La~ (3) in Ca-P coating and the soaking time t was calculated as follows: (?) Unit (PPB/mm~2). (3) was used to investigate the inhibitory effect of rare earth ions precipitated in Ca-P ceramic coating on osteoclast activity. The results showed that when the concentration of RANKL was 100 ng/mL,M-CSF and 50 ng/mL, the number of positive multinuclear osteoclasts induced was the highest. When the concentration of La~ (3) was 21 PPB, the number of osteoclasts induced was increased significantly (P0.05). Other concentrations of La~ (3) inhibited the number of osteoclasts induced. It can be concluded that when the content of La_2O_3 is 0.6 wt.%,Ca:P=1.40, the Ca-P ceramic coating not only has excellent microstructure and properties, but also has good bioactivity. The activity of osteoclast can be inhibited for about 16.5 years. It is expected that it can provide a new idea for the prevention and treatment of abrasion disease in clinical practice.
【学位授予单位】:贵州大学
【学位级别】:硕士
【学位授予年份】:2017
【分类号】:R318.08;TG174.4

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