宽带激光熔覆钙磷涂层中的稀土可控析出及其对破骨细胞活性的抑制作用
[Abstract]:Osteolysis of artificial joint (debris disease) is the main cause of artificial prosthesis loosening. The inhibition of osteoclast activity by rare earth ions will effectively prevent the formation of osteolysis. In this paper, the surface of biomedical metal material (Ti-6Al-4V) was modified by laser cladding, and the Ca-P bioceramics coating containing rare earth was prepared. The rare earth ions precipitated by the coating in physiological environment were used to prevent and cure osteolysis. Metallographic microscope (OM), scanning electron microscope (SEM), plasma mass spectrometer (ICP-AES), X ray diffractometer (XRD), microhardness instrument MTT colorimetric method inverted fluorescence microscope) was used. The interface of Ca-P bioceramics coating, micromorphology, precipitation regularity of rare earth ions, phase and microhardness of Ca-P bioceramics coating were examined by TRAP staining method. Biological activity and the inhibition of rare earth ion concentration on osteoclast activity were studied. The following conclusions were obtained: (1) the effects of different amounts of La_2O_3 doping and different Ca / P ratios on the microstructure, properties and biological activity of laser clad Ca-P ceramic coatings were investigated. The results show that the Ca-P ceramic coating mainly includes CaTiO_3,HA, 尾-TCP,TiO_2 phase. The higher the Ca:P ratio in the coating, the greater the average hardness. The doping amount of La_2O_3 is 0.6 wt.%,. The Ca-P ceramic coating of Ca:P=1.40 has the best microstructure, properties and biological activity. (2) the degradation and precipitation of Ca-P ceramic coating in simulated body fluid was investigated. The results show that the quality of Ca-P ceramic coating changes dynamically in simulated body fluid, which is a dynamic change of the coexistence of bone-like apatite deposition and 尾-TCP degradation. The average daily precipitation concentration of La~ (3) in the coating fluctuates from 9.5 to 12 PPB. The formula between the concentration C of La~ (3) in Ca-P coating and the soaking time t was calculated as follows: (?) Unit (PPB/mm~2). (3) was used to investigate the inhibitory effect of rare earth ions precipitated in Ca-P ceramic coating on osteoclast activity. The results showed that when the concentration of RANKL was 100 ng/mL,M-CSF and 50 ng/mL, the number of positive multinuclear osteoclasts induced was the highest. When the concentration of La~ (3) was 21 PPB, the number of osteoclasts induced was increased significantly (P0.05). Other concentrations of La~ (3) inhibited the number of osteoclasts induced. It can be concluded that when the content of La_2O_3 is 0.6 wt.%,Ca:P=1.40, the Ca-P ceramic coating not only has excellent microstructure and properties, but also has good bioactivity. The activity of osteoclast can be inhibited for about 16.5 years. It is expected that it can provide a new idea for the prevention and treatment of abrasion disease in clinical practice.
【学位授予单位】:贵州大学
【学位级别】:硕士
【学位授予年份】:2017
【分类号】:R318.08;TG174.4
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