石墨烯掺杂PLA纳米纤维膜的研究
发布时间：2019-03-13 10:38
【摘要】：皮肤是人体的重要组成部分,它与我们的体温控制,人体免疫力以及人体与外界的传感等重要功能息息相关。创伤是我们不可忽视的一类疾病,伤口敷料一直是生活以及医药领域的必需品。传统敷料已不再满足人们的要求,所以近年来,可降解性合成聚合物纳米纤维敷料成为大家研究的重点。聚乳酸(PLA)有着难以比拟的优良生物降解性、生物相容性和一定的强度,广泛应用于生物医药领域。近来,有关石墨烯的抗菌性以及其细胞毒性的研究很多,结果表明石墨烯具有很好的抑菌效果且对细胞无毒性,同时氧化石墨烯有着很好的亲水性能,这为石墨烯在生物医药领域的应用提供了坚实的理论基础。目前为止,尚未发现有关将石墨烯加入聚乳酸进行静电纺丝,应用于生物伤口敷料的相关报道。本论文采用静电纺丝的方法制备出石墨烯改性聚乳酸(PLA)纳米纤维膜,探究石墨烯对纳米纤维膜性能的影响,探索其在生物伤口敷料应用潜力。首先,我们用层间催化解离法(Interlayer catalytic exfoliation,ICE)制备石墨烯,并对石墨烯进行SEM、TEM、FT-IR、Raman、XRD等测试。测试结果表明石墨烯具有较大的尺寸,而且厚度小于2nm。最终得到的石墨烯几乎没有缺陷,保留了其原有的完整基本晶面。接着,我们对纯的PLA进行静电纺丝工艺的探讨,得出如下结论:环境参数:温度为25℃,相对湿度为25%;纺丝液浓度;15%;过程工艺参数:电压为15kV喂料速率为0.5ml/h,针头到收集装置的水平距离为15cm;使用锡箔纸为最终收集装置。运用这一工艺参数纺制了石墨烯改性纳米纤维膜,对单根纤维进行了SEM以及TEM测试,结果表明石墨烯被包裹在纤维内部。对纳米纤维膜进行了FT-IR、Raman以及XPS测试,再次表明膜中含有石墨烯。最后,我们对复合膜的抗菌性能进行了测试。先对石墨烯及氧化石墨烯进行了抑菌率测试,结果发现石墨烯几乎不抗菌,而氧化石墨烯的抑菌率大于90%。然后分别对掺杂石墨烯与氧化石墨烯的静电纺丝膜进行抑菌圈实验,结果发现掺杂石墨烯的静电纺丝膜没有出现抑菌环,而掺杂氧化石墨烯的静电纺丝膜出现了一小圈的抑菌环,但是很小,可能是由于膜中氧化石墨烯含量太少导致的。为将其应用在敷料方面,需要对其进行更加深入的探索研究。
[Abstract]:Skin is an important part of the human body, it is closely related to our temperature control, human immunity and human body and external sensors and other important functions. Trauma is a kind of disease that we can't ignore. Wound dressing has always been a necessity of life and medicine. Traditional dressings no longer meet the needs of people, so in recent years, degradable synthesis of polymer nanofiber dressings has become the focus of research. Poly (lactic acid) (PLA) is widely used in biomedicine because of its incomparable biodegradability, biocompatibility and certain strength. Recently, there have been a lot of researches about the antibacterial activity and cytotoxicity of graphene. The results show that graphene has good bacteriostatic effect and no toxicity to cells, and that graphene oxide has good hydrophilicity, and that graphene has good bacteriostatic effect and no toxicity to cells. This provides a solid theoretical basis for the application of graphene in the field of biomedicine. So far, it has not been reported that graphene was added to polylactic acid for electrospinning and applied to biological wound dressing. In this paper, graphene-modified polylactic acid (PLA) nano-fiber membrane was prepared by electrospinning, and the effect of graphene on the performance of nano-fiber membrane was investigated, and the potential application of graphene in bio-wound dressing was explored. Firstly, we prepared graphene by interlayer catalytic dissociation method (Interlayer catalytic exfoliation,ICE), and tested graphene by SEM,TEM,FT-IR,Raman,XRD and so on. The results show that graphene has a large size and a thickness of less than 2 nm. The resulting graphene has almost no defects and retains its original basic crystal plane. Then, we discuss the electrospinning process of pure PLA and draw the following conclusions: the temperature is 25 鈩，
本文编号：2439313
[Abstract]:Skin is an important part of the human body, it is closely related to our temperature control, human immunity and human body and external sensors and other important functions. Trauma is a kind of disease that we can't ignore. Wound dressing has always been a necessity of life and medicine. Traditional dressings no longer meet the needs of people, so in recent years, degradable synthesis of polymer nanofiber dressings has become the focus of research. Poly (lactic acid) (PLA) is widely used in biomedicine because of its incomparable biodegradability, biocompatibility and certain strength. Recently, there have been a lot of researches about the antibacterial activity and cytotoxicity of graphene. The results show that graphene has good bacteriostatic effect and no toxicity to cells, and that graphene oxide has good hydrophilicity, and that graphene has good bacteriostatic effect and no toxicity to cells. This provides a solid theoretical basis for the application of graphene in the field of biomedicine. So far, it has not been reported that graphene was added to polylactic acid for electrospinning and applied to biological wound dressing. In this paper, graphene-modified polylactic acid (PLA) nano-fiber membrane was prepared by electrospinning, and the effect of graphene on the performance of nano-fiber membrane was investigated, and the potential application of graphene in bio-wound dressing was explored. Firstly, we prepared graphene by interlayer catalytic dissociation method (Interlayer catalytic exfoliation,ICE), and tested graphene by SEM,TEM,FT-IR,Raman,XRD and so on. The results show that graphene has a large size and a thickness of less than 2 nm. The resulting graphene has almost no defects and retains its original basic crystal plane. Then, we discuss the electrospinning process of pure PLA and draw the following conclusions: the temperature is 25 鈩，
本文编号：2439313
本文链接：https://www.wllwen.com/yixuelunwen/swyx/2439313.html
