力学增强型水凝胶纤维的制备及其生物相容性评价

发布时间：2019-03-18 14:48

【摘要】：针对天然水凝胶纤维力学性能及成形性能差的问题,结合微流控和光交联的方法,高通量制备了一种力学增强型海藻酸钠/明胶甲基丙烯酸酯复合水凝胶材料。研究了微流控喷头尺寸、水凝胶单体浓度对水凝胶纤维成形性能、水凝胶力学特性及结构特征的影响。结果表明:制备的水凝胶纤维边缘光滑,直径(390±50μm)较为均一,具有良好的操作性及可控性;随着海藻酸钠和明胶甲基丙烯酸酯单体浓度的增高,水凝胶弹性模量升高,SEM结果显示其片层微结构更为致密。进一步测试了水凝胶纤维的生物相容性,细胞实验结果表明,培养至第5天时,水凝胶中细胞的存活率稳定维持在80%以上,证明所制备的水凝胶具有良好的生物相容性,适宜细胞生长。动物实验结果表明,当凝胶植入动物体内1周后,周围的皮下组织很正常;在第3周时,凝胶周围的炎症反应明显减轻,材料也出现一定程度的降解。研究力学增强型海藻酸钠/明胶甲基丙烯酸酯复合水凝胶,有望于实现三维肌肉微组织模型的高通量构建。
[Abstract]:Aiming at the problems of poor mechanical property and forming property of natural water gel fiber, a mechanical enhanced sodium alginate/ gelatin methacrylate composite hydrogel material is prepared in high flux by combining micro-fluidic and light cross-linking methods. The effect of the size of the micro-fluidic spray head, the concentration of the water-gel monomer on the forming property of the hydrogel fiber, the mechanical properties and the structural characteristics of the hydrogel was studied. The results show that the edge of the prepared hydrogel fiber is smooth and the diameter (390 to 50. mu.m) is uniform, and has good operability and controllability; with the increase of the concentration of the sodium alginate and the gelatin methacrylate monomer, the elastic modulus of the hydrogel is increased, The SEM results show that the lamellar microstructure is more compact. The biocompatibility of the hydrogel fiber was further tested. The results of the cell experiment showed that the survival rate of the cells in the hydrogel was maintained at more than 80% on the 5th day, and it was proved that the prepared hydrogel had good biocompatibility and suitable for cell growth. The results of animal experiments show that the surrounding tissue of the surrounding subcutis is normal after the gel is implanted in the animal for 1 week; at the third week, the inflammatory response around the gel is obviously reduced, and the material also has a certain degree of degradation. The research on the mechanical enhanced sodium alginate/ gelatin methacrylate composite hydrogel is expected to be used to realize the high-throughput construction of the three-dimensional muscle micro-tissue model.
【作者单位】：西安交通大学生命科学与技术学院;马鞍山师范高等专科学校;西安交通大学生物医学工程与生物力学研究中心;安徽中医药高等专科学校;
【基金】：2017年陕西省自然科学基础研究计划资助项目(2017JQ1036) 安徽高校自然科学研究重点资助项目(KJ2017A681) 博士后创新人才支持计划资助项目(2016)
【分类号】：R318.08;TQ340.1

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