有序排列PMMA电纺纳米纤维在引导大鼠背根神经元生长中的价值探讨

发布时间：2019-03-22 20:28

【摘要】：神经损伤后可植入性组/细胞支架设计是周围神经损伤、和脊髓损伤后神经修复一个重要思路。本研究利用静电纺丝技术成功构建具有随或有序拓扑结构聚甲丙烯酸甲酯（PMMA）纳米纤维材料，并进行大鼠原代背根神经节神经元（DRGn）培养，及其与大鼠原代雪旺细胞（SCs）共培养。结果显示所构建PMMA电纺纳米纤维具有良好生物相容性，适合进行神经细胞培养；通过所转染绿色荧光蛋白表达，发现DRGn能够顺利在电纺纤维上贴壁并生长，电纺纤维拓扑结构能够影响DRGn生长能力和生长方向，DRGn在有序电纺纳米纤维上能够生成更长神经突，且神经突生长方向能够和及质纤维延伸方向保持高度一致；通过进一步分析及扫描电镜检查，发现不管在随或有序PMMA电纺纳米纤维，DRGn神经突均能够沿纤维行，，但相比随纤维，有序电纺纤维对DRGn引导能力更强，DRGn似乎更倾向于循有序纤维生长；通过DRGn与SCs共培养，则发现较PMMA薄膜和随电纺纤维，有序电纺纤维能够在形态上促成DRGn与SCs建立更多共定位，估计能够促进后续髓鞘化进程。本课研究结果示有序PMMA电纺纳米纤维具有作为神经损伤后植入性支架力，值后续更进一步体内研究。
[Abstract]:The design of implantable group / cell scaffold after nerve injury is an important idea for peripheral nerve injury and nerve repair after spinal cord injury. In this study, poly (methyl methacrylate) (PMMA) nanofibers with random or ordered topological structure were successfully constructed by electrospinning technique, and the primary dorsal root ganglion neurons (DRG) of rats were cultured with (DRGn). And co-cultured with rat primary Schwann cells (SCs). The results showed that the PMMA electrospun nanofibers had good biocompatibility and were suitable for nerve cell culture. Through the expression of green fluorescent protein, it was found that DRGn could attach and grow on electrospun fibers smoothly. The topological structure of electrospun fibers could affect the growth ability and growth direction of DRGn, and DRGn could produce longer nerve processes on ordered electrospun nanofibers. And the direction of neurite growth was highly consistent with the direction of fiber extension. By means of further analysis and scanning electron microscopy, it was found that the DRGn nerve processes were able to walk along the fibers, regardless of whether they were electrospun with or without PMMA, but the ordered electrospun fibers had stronger ability to guide DRGn than with fibers. DRGn seems to be more inclined to follow the order of fiber growth; By co-culture of DRGn and SCs, it was found that the ordered electrospun fibers could promote more co-localization of DRGn and SCs in morphology than those of PMMA thin films and electrospun fibers, and could promote the subsequent myelinization process. The results of this study show that ordered PMMA electrospun nanofibers have implantable scaffold force after nerve injury and further study in vivo.
【学位授予单位】：重庆医科大学
【学位级别】：博士
【学位授予年份】：2014
【分类号】：R651.3;R318.08

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