新型组织工程化改性胶原-壳聚糖复合支架制备与神经细胞的相容性

发布时间：2019-04-12 19:28

【摘要】：背景:周围神经缺损的治疗在再生医学领域仍然具有挑战性,支架材料的应用被认为是有前途的探索方向之一,理想的支架材料应是安全、耐用和缺乏抗原性的。目的:探讨制备一种新型的组织工程化改性胶原-壳聚糖支架,并探索其在大鼠横断坐骨神经模型中用作桥梁修复神经损伤的可能。方法:先制备出胶原-壳聚糖复合支架,然后用1-乙基-3-(3-二甲基氨基丙基)-碳化二亚胺,N-羟基琥珀酰亚胺对支架进行化学改性即交联。接着将神经干细胞种植于支架上,之后连同支架-种子细胞在体内植入坐骨神经缺损部位。结果与结论:制得的变性交联胶原-壳聚糖复合支架具有三维立体网状结构。将支架-种子细胞移植4周后,可在损伤的坐骨神经中发现S-100神经丝蛋白200表达。结果表明这种组织工程化改性交联胶原-壳聚糖复合支架具有良好的细胞相容性。
[Abstract]:Background: the treatment of peripheral nerve defects is still challenging in regenerative medicine. The application of stent materials is considered to be one of the promising exploration directions. The ideal scaffold materials should be safe, durable and lack of antigenicity. Aim: to study the preparation of a new type of tissue engineering modified collagen chitosan scaffold and explore the possibility of using it as a bridge to repair nerve injury in rat sciatic nerve model. Methods: collagen-chitosan composite scaffolds were prepared, and then the scaffolds were chemically modified with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide and N-hydroxysuccinimide. Neural stem cells were then implanted on the scaffold, and then together with the scaffold-seed cells, the sciatic nerve defect was implanted into the body. Results & conclusion: the modified cross-linked collagen-chitosan composite scaffolds have three-dimensional reticular structure. Four weeks after transplantation of scaffold-seeded cells, the expression of neurofilament 200 was found in injured sciatic nerve. 4 weeks after transplantation, the expression of neurofilament 200 was found in the injured sciatic nerve. The results showed that the cross-linked collagen-chitosan composite scaffolds with tissue engineering had good cellular compatibility.
【作者单位】：湖北医药学院第一临床学院;湖北医药学院麻醉学研究所;湖北医药学院附属太和医院麻醉科;湖北医药学院基础医学研究所;
【基金】：国家教育部2012年度国家级大学生创新创业训练计划项目(201210929004) 国家教育部2016年度国家级大学生创新创业训练计划项目(201610929005) 十堰市科学技术研究与开发项目(14Y13) 2016年度湖北医药学院教学研究项目(2016027) 2016年度湖北医药学院附属太和医院科学研究基金(2016JJXM001) 湖北医药学院高层次人才科研启动金(2011QDZR-1,2011QDZR-2) 湖北医药学院2015年度“十二五规划”学科建设学科英才成长资助项目(2015)~~
【分类号】：R318.08

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