PLGA、壳聚糖纳米粒的制备及细胞毒性研究

发布时间：2019-04-26 16:55

【摘要】：目的:制备大小均一的PLGA、壳聚糖纳米粒并研究其对牙周膜细胞的毒性。方法:采用乳化溶剂挥发法制备PLGA纳米粒;离子交联法制备壳聚糖纳米粒;用Zetasizer 3000 HS检测纳米粒的粒径和分散系数,透射电镜观察纳米粒的形态。取3~5代人牙周膜细胞,分别与0(对照组)、0.5、1、2 mg/m L的PLGA和壳聚糖纳米粒以及1 mg/m L普通粒径的PLGA和壳聚糖粉末共同培养;分别于培养24、48、72 h后用MTT法检测各组细胞的相对增殖率,评价PLGA、壳聚糖纳米粒的细胞毒性。结果:透射电镜观察:PLGA纳米粒呈球形,壳聚糖纳米粒类似球形,两者大小较均匀、边缘清晰。Zetasizer 3000HS检测显示:PLGA纳米粒的粒径为(188.10±2.86)nm,分散系数为0.55±0.04;壳聚糖纳米粒的粒径为(250.97±30.86)nm,分散系数为0.50±0.07。毒性实验结果:培养48 h时,PLGA、壳聚糖粉末组以及2 mg/m L PLGA、1 mg/m L壳聚糖纳米粒组的细胞增殖率均低于空白对照组(P0.05),其他各时间点各组间均无统计学差异(P0.05);各组各时间点的细胞相对增殖率均大于85%,细胞毒性为0或1级。结论:根据ISO10993-5,PLGA和壳聚糖纳米粒对牙周膜细胞无毒性。
[Abstract]:Aim: to prepare homogeneous PLGA, chitosan nanoparticles and study their toxicity to periodontal ligament cells. Methods: PLGA nanoparticles were prepared by emulsifying solvent volatilization method, chitosan nanoparticles were prepared by ion cross-linking method, the size and dispersion coefficient of nanoparticles were detected by Zetasizer 3000 HS, and the morphology of nanoparticles was observed by transmission electron microscopy (TEM). Human periodontal ligament cells were cultured with 0 (control group), 0.5, 1, 2 mg/m / L PLGA and chitosan nanoparticles, 1 mg/m / L PLGA and chitosan powder, respectively. After 24, 48 and 72 hours of culture, the relative proliferation rate of PLGA, chitosan nanoparticles was detected by MTT assay, and the cytotoxicity of chitosan nanoparticles was evaluated. Results: the results of transmission electron microscopy showed that PLGA nanoparticles were spherical and chitosan nanoparticles were spherical. The size of PLGA nanoparticles was uniform and the edges were clear. Zetasizer 3000HS analysis showed that the dispersion coefficient of PLGA nanoparticles was (188.10 卤2.86) nm, and the dispersion coefficient was 0.55 卤0.04.Zetasizer 3000HS analysis showed that the dispersion coefficient of PLGA nanoparticles was (188.10 卤2.86) nm,. The particle size of chitosan nanoparticles was (250.97 卤30.86) nm, and the dispersion coefficient was 0.50 卤0.07. The results of toxicity test showed that the proliferation rate of PLGA, chitosan powder group and 2 mg/m / L PLGA,1 mg/m L chitosan nanoparticles group was lower than that of blank control group at 48 h (P0.05). There was no statistical difference between each group at other time points (P0.05). The relative proliferation rate of each group was more than 85% at each time point, and the cytotoxicity was 0 or 1 grade. Conclusion: according to ISO10993-5,PLGA and chitosan nanoparticles, there is no toxicity to periodontal ligament cells.
【作者单位】：南方医科大学南方医院·南方医科大学口腔医学院;
【基金】：广东省科技计划项目(2010B060900053)
【分类号】：R318.08

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