Lys调节PLGA纳米纤维的体外降解产物酸度的研究
发布时间：2019-05-10 02:22
【摘要】：采用向PLGA纳米纤维中添加碱性氨基酸——赖氨酸(Lys)的方法来降低降解产物的集酸程度.用电纺丝技术制备载Lys的PLGA/Lys复合纳米纤维,并用SEM、DSC、及力学测试等表征手段对该复合纳米纤维的形貌、热学性质以及力学性质等方面进行表征;研究PLGA/Lys复合纳米纤维中Lys的释放特性;对PLGA与PLGA/Lys纳米纤维进行体外降解实验,评价纤维降解过程中的形貌和降解液pH值;用已降解8周的纳米纤维降解液对血管平滑肌细胞进行培养,MTT检测细胞毒性.结果表明,在电压为12 kV,注射速率1.0mL/h,温度23℃,湿度20%,接收距离14 cm时可以纺出形貌较好的PLGA/Lys复合纳米纤维,Lys含量越多,复合纳米纤维的直径越细.纳米纤维中Lys的含量影响Lys的释放特性.Lys促进PLGA的降解并对改善其降解产物的酸度有一定的缓解作用,含2%Lys的纳米纤维降解8周后可以使降解液pH维持在7.4~6.7之间.降解的酸性产物不利于平滑肌细胞生长,但含Lys降解液的培养基中细胞生长状态良好,这可能是由于Lys能减轻酸性降解产物对细胞功能的影响,同时也能为细胞增殖提供营养物质.
[Abstract]:The alkaline amino acid-lysine (Lys) was added to the PLGA nanofibers to reduce the acid concentration of the degradation products. PLGA/Lys composite nanofibers loaded with Lys were prepared by electrospinning technique. The morphology, thermal properties and mechanical properties of the composite nanofibers were characterized by SEM,DSC, and mechanical tests. The release characteristics of Lys from PLGA/Lys composite nanofibers were studied, and the degradation experiments of PLGA and PLGA/Lys nanofibers in vitro were carried out to evaluate the morphology and pH value of the degradation solution. Vascular smooth muscle cells (VSMCs) were cultured with biodegradable nanofiber solution for 8 weeks. The cytotoxicity of vascular smooth muscle cells (VSMCs) was detected by MTT. The results show that the PLGA/Lys composite nanofibers with good morphology can be spun at 12 kV, injection rate 1.0 mL 路h, temperature 23 鈩，
本文编号：2473273
[Abstract]:The alkaline amino acid-lysine (Lys) was added to the PLGA nanofibers to reduce the acid concentration of the degradation products. PLGA/Lys composite nanofibers loaded with Lys were prepared by electrospinning technique. The morphology, thermal properties and mechanical properties of the composite nanofibers were characterized by SEM,DSC, and mechanical tests. The release characteristics of Lys from PLGA/Lys composite nanofibers were studied, and the degradation experiments of PLGA and PLGA/Lys nanofibers in vitro were carried out to evaluate the morphology and pH value of the degradation solution. Vascular smooth muscle cells (VSMCs) were cultured with biodegradable nanofiber solution for 8 weeks. The cytotoxicity of vascular smooth muscle cells (VSMCs) was detected by MTT. The results show that the PLGA/Lys composite nanofibers with good morphology can be spun at 12 kV, injection rate 1.0 mL 路h, temperature 23 鈩，
本文编号：2473273
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