自组装膜表面不同功能团对骨骼肌细胞生物学特性的影响

发布时间：2019-05-10 07:25

【摘要】：目的探讨自组装膜表面不同的功能团对骨骼肌细胞生物学特性影响。方法通过自组装技术在金片表面接枝不同的化学功能团(-CH3、-COOH、-NH2、-OH),测定接触角和原子力显微镜确定其表征,将材料与骨骼肌细胞进行共培养,分别通过SEM、DAPI染色、MTT法、钙黄绿素染色和流式检测法等方法观察不同功能团对骨骼肌细胞的形态、粘附、增殖、活力及凋亡的影响。结果含有-NH2和-COOH基团的材料表面能明显促进骨骼肌的粘附、增殖,具有良好的生物相容性;而含有-CH3基团的基片明显不利于骨骼肌细胞的粘附、增殖,并对骨骼肌细胞凋亡有一定的促进;不同的功能团材料对骨骼肌细胞粘附、增殖率影响的顺序从大到小依次为-NH2-COOH-OH-CH3;不同的化学功能团对细胞凋亡率和毒性的顺序-NH2-COOH-OH-CH3。结论不同的功能团对骨骼肌细胞的生物特性具有不同的影响,含有-NH2基团的基片能明显促进细胞的粘附和增殖,有利于构建具有促细胞粘附的生物材料界面;而含有-CH3基团的材料不利于细胞的粘附和增殖,有利于构建抗粘附的生物材料表面界面。
[Abstract]:Objective to investigate the effects of different functional groups on the biological characteristics of skeletal muscle cells. Methods different chemical functional groups (- CH3,-COOH,-NH2,-OH) were grafting on the surface of gold sheet by self-assembly technique. The contact angle and atomic force microscope were measured and characterized. The material was co-cultured with skeletal muscle cells and SEM, respectively. The effects of different functional groups on the morphology, adhesion, proliferation, activity and apoptosis of skeletal muscle cells were observed by DAPI staining, MTT staining, calcium green staining and flow cytometry. Results the surface of the material containing-NH2 and-COOH groups could obviously promote the adhesion and proliferation of skeletal muscle, and had good biocompatibility. However, the substrate containing-CH3 group was not conducive to the adhesion and proliferation of skeletal muscle cells, and promoted the apoptosis of skeletal muscle cells to a certain extent. The order of the effect of different functional groups on the adhesion and proliferation rate of skeletal muscle cells from large to small was-the order of apoptosis rate and toxicity of different chemical functional groups of NH2-COOH-OH-CH3;-NH2-COOH-OH-CH3.. Conclusion different functional groups have different effects on the biological characteristics of skeletal muscle cells. The substrate containing-NH2 group can obviously promote the adhesion and proliferation of skeletal muscle cells, which is beneficial to the construction of biomaterial interface with cell adhesion. However, the material containing-CH3 group is not conducive to the adhesion and proliferation of cells, and is beneficial to the construction of anti-adhesion biomaterial surface interface.
【作者单位】：南方医科大学研究生院;广州军区广州总医院骨科医院;清华大学材料科学与工程系;
【基金】：国家自然科学基金(2012022) 全军医学科学技术研究“十二五”计划面上项目(cws11c268) 广东省科技计划项目(2012A030400064)~~
【分类号】：R318.08

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