新型骨支架复合材料修复牙槽骨缺损的研究

发布时间：2019-05-10 19:11

【摘要】：目的通过多孔聚丙烯晴基碳纤维/壳聚糖/双相磷酸钙/聚乳酸-羟基乙酸(PAN/CS/BCP/PLGA)复合支架的实验研究，探讨该复合支架对大白兔牙槽骨缺损的修复能力。材料与方法采用湿法合成双相磷酸钙,真空冷冻干燥技术制备多孔PAN/CS/BCP/PLGA复合支架,测定抗压强度、阿基米德法测定孔隙率及体外模拟生物活性，并采用XRD、SEM分析相结构及形貌特征。选取32只成年雄性新西兰大白兔，随机分为实验组和对照组，手术制备大小为10mm×10mm×3mm牙槽骨缺损区。实验组骨缺损区植入PAN/CS/BCP/PLGA复合支架，对照组植入CS/PLGA复合材料，术后2、4、8、12周分期取材，进行X线检查、组织学观察、扫描电镜(SEM)检测和成骨细胞计数分析。结果PAN/CS/BCP/PLGA复合支架中添加4wt%PAN后，平均孔隙率大于85%,抗压强度大于5.9MPa，提高了6倍，呈现多孔贯通形貌，孔径平均为400-500μm,PAN分布均匀。术后12周实验组检测结果，X线观察：骨缺损区阻射影与自体骨相同。组织学观察：骨缺损区完全被新骨充填，且为较成熟的板层骨，与自体骨完全融合。SEM检测：骨-材料界面形成骨性结合，移植材料大部分降解，被成熟骨组织替代。成骨细胞计数分析：成骨细胞生长活跃，统计学有显著性差异。结论多孔PAN/CS/BCP/PLGA复合支架具备良好的生物活性和生物可降解性,对骨缺损的修复能力要优于CS/PLGA复合材料，为其成为骨组织工程支架替代材料提供理论依据。
[Abstract]:Aim to investigate the ability of porous polypropylene-based carbon fiber / chitosan / biphasic calcium phosphate / poly (lactic acid-glycolic acid) (PAN/CS/BCP/PLGA) composite scaffolds to repair alveolar bone defects in rabbits. Materials and methods two-phase calcium phosphate was synthesized by wet method, porous PAN/CS/BCP/PLGA composite scaffolds were prepared by vacuum freeze-drying technique, compressive strength was measured, porosity and simulated biological activity in vitro were measured by Archimedes method, and XRD, was used. The phase structure and morphology were analyzed by SEM. Thirty-two adult male New Zealand white rabbits were randomly divided into experimental group and control group. The size of alveolar bone defect area was 10mm 脳 10mm 脳 3mm. In the experimental group, PAN/CS/BCP/PLGA composite scaffold was implanted in bone defect area, and CS/PLGA composite material was implanted in the control group. After 2, 4, 8 and 12 weeks after operation, the materials were collected in stages for X-ray examination, histological observation, scanning electron microscope (SEM) detection and osteoblast count analysis. Results after adding 4wt%PAN to the PAN/CS/BCP/PLGA composite scaffolds, the average porosity was more than 85%, the compressive strength was greater than 5.9 MPA, and the average pore size was 400-500 渭 m, and the distribution of PAN was uniform. The average pore size was 400-500 渭 m and the compressive strength was more than 5.9 MPA, which was increased by 6 times. 12 weeks after operation, the results of X-ray observation showed that the blocking radiography of bone defect area was the same as that of autogenous bone. Histological observation: the bone defect area was completely filled with new bone, and it was a mature lamellar bone, and completely fused with autogenous bone. SEM showed that the bone-material interface formed bony union, most of the graft materials were degraded, and were replaced by mature bone tissue. Osteoblast count analysis: osteoblasts grew actively, and there was significant difference between them. Conclusion porous PAN/CS/BCP/PLGA composite scaffolds have good biological activity and biodegradability, and their ability to repair bone defects is better than that of CS/PLGA composites, which provides a theoretical basis for them to become substitute materials for bone tissue engineering scaffolds.
【学位授予单位】：佳木斯大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R318.08

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