结构仿生型骨骼修复支架材料的研究

发布时间：2019-05-21 10:16

【摘要】：本论文致力于构建具有一定延展性、机械强度、高比表面积、良好生物相容性、优异生物活性、可渗透的多孔结构的钛基钛酸盐纳米线生物支架材料，探讨其制备机理、性质特征，探索其作为生物体植入装置应用的可能性。论文首先综述了生物支架材料最新研究进展，，简单介绍了生物支架材料研究领域所存在的关键问题。在总结已有研究成果的基础上，提出本论文的研究设想和新型生物医用支架材料的制备方案。论文通过水热合成法制备网格状微孔结构钛基钛酸盐纳米线基体材料，在类体液(SBF)体系中，采用电化学沉积技术，以上述基体材料为工作电极、铂电极为对极、甘汞电极为参比电极，通过电流协助Ca2+、PO3-4与OH-相互作用反应得到羟基磷酸钙(HA)纳米颗粒修饰的钛基钛酸盐纳米线支架材料。采用场发射扫描电子显微镜(FESEM)、透射电子显微镜(TEM)、X-射线衍射光谱(XRD)、傅立叶—衰减全反射红外光谱(FTIR-ATR)等手段表征了此支架材料的结构。结果表明，所制得的“项链珠模型”核壳结构的HA纳米颗粒修饰钛基钛酸盐纳米线支架材料与生物体自然骨结构和功能相近，其中羟基磷酸钙纳米颗粒的长约150nm、宽约30nm，且结构为六方晶系，此种材料在一定程度上达到了结构功能化仿生的目的。通过体外细胞培养进一步对比研究了人体造骨细胞MG63在HA纳米颗粒修饰前后钛基钛酸盐纳米线支架材料表面上的吸附、接触、生长和分化现象，得到HA纳米颗粒的定向修饰有利于材料生物相容性和生物活性的进一步提高。论文以水热合成技术得到的网格状微孔结构钛基钛酸盐纳米线基体材料为基础，在Hummers、Offeman法得到2.0mg/mL的石墨烯纳米片悬浮液体系中，采用电泳沉积技术，以上述基体材料为工作电极、铂电极为对极，通过电流协助石墨烯纳米片与钛酸盐纳米线相互作用得到石墨烯纳米片修饰的钛基钛酸盐纳米线支架材料。采用场发射扫描电子显微镜(FESEM)、X-射线衍射光谱(XRD)、拉曼光谱(Raman)、傅立叶—衰减全反射红外光谱(FTIR-ATR)、荧光光谱(PL)、紫外—可见光谱(UV-vis)等手段表征了此支架材料的结构。结果表明，随着电泳沉积时间的延续，钛酸盐纳米线基体材料上所修饰石墨烯纳米片的形貌主要是从有规律的相互重叠到无规律的相互堆积的过程，且钛基钛酸盐纳米线的网格状微孔结构逐渐的遭到破坏。通过体外细胞培养进一步研究了人体造骨细胞MG63在石墨烯纳米片修饰前后的钛基钛酸盐纳米线支架材料表面上的吸附、接触、生长和分化现象，得到石墨烯纳米片的有规律的修饰有利于材料生物性能的改善。最后，总结了论文所做的具体工作，并提出了论文中有待继续解决的问题，为进一步的研究工作明确了方向。
[Abstract]:The purpose of this paper is to construct titanium based titanate nanowires with porous structure with certain extensibility, mechanical strength, high specific surface area, good biocompatibility, excellent biological activity and permeable porous structure, and to explore the preparation mechanism of titanium based titanate nanowires with certain extensibility, mechanical strength, high specific surface area, good biocompatibility, excellent biological activity and permeable porous structure. To explore the possibility of its application as an organism implantation device. In this paper, the latest research progress of biological scaffold materials is reviewed, and the key problems in the research field of biological scaffold materials are briefly introduced. On the basis of summing up the existing research results, the research ideas of this paper and the preparation scheme of new biomedical scaffold materials are put forward. In this paper, the matrix material of lattice microporous titanium based titanate nanowires was prepared by hydrothermal synthesis. In the humoral (SBF) system, electrochemical deposition technology was used as the working electrode and platinum electrode was used. Calomel electrode was used as reference electrode to prepare titanium based titanate nanowires modified by calcium hydroxyphosphate (HA) nanoparticles by current-assisted interaction of Ca2, PO3-4 and OH-. The structure of the scaffold was characterized by field emission scanning electron microscope (FESEM), transmission electron microscope (TEM), X-ray diffraction spectrum (XRD), Fourier-attenuated total reflection infrared spectroscopy (FTIR-ATR). The results show that the structure and function of titanium based titanate nanowires modified by HA nanoparticles with "necklace bead model" core-shell structure are similar to those of natural bone, in which calcium hydroxyphosphate nanoparticles are about 150 nm long and 30 nm wide. And the structure is hexagonal system, which achieves the purpose of structural functionalization bionic to a certain extent. The adsorption, contact, growth and differentiation of human osteoblasts MG63 on the surface of titanium based titanate nanowires before and after HA nanoparticles modification were further studied by cell culture in vitro. The directional modification of HA nanoparticles is beneficial to the further improvement of biocompatibility and biological activity of the materials. In this paper, based on the matrix material of reticulated microporous titanium based titanate nanowires obtained by hydrothermal synthesis, the graphene nanoparticles suspension system of 2.0mg/mL was obtained by Hummers, offemaner method, and the electrodeposition technique was used. Using the matrix material as working electrode and platinum electrode as opposite electrode, graphene nanowires modified titanium based titanate nanowires were prepared by the interaction of graphene nanowires with titanate nanowires. Field emission scanning electron microscope (FESEM), X-ray diffraction spectrum (XRD), Raman spectrum (Raman), Fourier-attenuated total reflection infrared spectrum (FTIR-ATR), fluorescence spectrum (PL), The structure of the scaffold was characterized by UV-vis spectroscopy (UV-vis). The results show that the morphology of graphene nanoparticles modified on titanate nanowires is mainly from regular overlap to irregular stacking with the prolongation of electrophoresis deposition time. The meshed microporous structure of titanium-based titanate nanowires was gradually destroyed. The adsorption, contact, growth and differentiation of human osteoblasts MG63 on the surface of titanium based titanate nanowires before and after graphene nanoparticles modification were further studied by cell culture in vitro. The regular modification of graphene nanoparticles is beneficial to the improvement of biological properties of graphene nanoparticles. Finally, the specific work of the paper is summarized, and the problems to be solved in the paper are put forward, which clarifies the direction of further research work.
【学位授予单位】：浙江理工大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：TB383.1;R318.08

【共引文献】