含锌镁合金对人成骨肉瘤U2OS细胞株体外增殖的影响

发布时间：2019-05-22 14:53

【摘要】：背景:合金化可提高纯镁的抗腐蚀性能,延缓其降解速率;大量文献表明锌具有良好的抗肿瘤效果。目的:评价含锌镁合金对人成骨肉瘤U2OS细胞体外增殖及凋亡的影响。方法:检测纯镁及不同含锌量镁合金(质量分数2%、4%、6%)在Hank’s液中的氢气释放速率。取人成骨肉瘤U2OS细胞(或MC3T3-E1细胞),分别与不同含锌量镁合金浸提液、纯镁浸提液及钛合金浸提液共培养1,3,5 d,MTT法检测细胞增殖。取人成骨肉瘤U2OS细胞,分别与不同含锌量镁合金浸提液、纯镁浸提液及钛合金浸提液共培养24 h,流式细胞仪分析细胞凋亡,Western Blotting检测凋亡相关蛋白的表达水平。结果与结论:①在初始的100 h内,纯镁组氢气释放速率明显高于含锌镁合金组;在不同含锌量镁合金组中,以含锌量4%镁合金组氢气释放速率最慢;②不同含锌量镁合金均可明显抑制人成骨肉瘤U2OS细胞的增殖,且与锌含量呈正相关;不同含锌量镁合金对MC3T3-E1细胞的毒性在0至1级之间;③不同含锌量的镁合金可明显促进人成骨肉瘤U2OS细胞的凋亡,且与锌含量呈正相关;④不同含锌量镁合金可上调人成骨肉瘤U2OS细胞p53、Bax蛋白表达,下调Bcl-2蛋白表达,改变Bcl-2/Bax比例,其中以含锌量4%镁合金效果最显著;⑤结果表明:含锌镁合金可抑制人成骨肉瘤U2OS细胞的体外增殖,诱导其凋亡。
[Abstract]:Background: alloying can improve the corrosion resistance and delay the degradation rate of pure magnesium, and a large number of literatures show that zinc has good antitumor effect. Aim: to evaluate the effect of zinc magnesium alloy on proliferation and apoptosis of human osteosarcoma U2OS cells in vitro. Methods: the hydrogen release rate of pure magnesium and magnesium alloys with different zinc content (mass fraction 2%, 4%, 6%) in Hank' s solution was measured. Human osteosarcoma U2OS cells (or MC3T3-E1 cells) were co-cultured with magnesium alloy extract with different zinc content, pure magnesium extract and titanium alloy extract for 1 day and 5 days, respectively. the proliferation of human osteosarcoma cells was detected by MC3T3-E1 assay. Human osteosarcoma U2OS cells were co-cultured with magnesium alloy extract with different zinc content, pure magnesium extract and titanium alloy extract for 24 H. the expression of apoptosis-related proteins was detected by flow cytometry., Western Blotting was used to detect the expression of apoptosis-related proteins. Results and conclusion: 1 the hydrogen release rate of pure magnesium group was significantly higher than that of zinc magnesium alloy group within the initial 100 h, and the hydrogen release rate of zinc content 4% magnesium alloy group was the slowest in different zinc content magnesium alloy group, and the hydrogen release rate of pure magnesium alloy group was significantly higher than that of zinc magnesium alloy group, and the hydrogen release rate of zinc content 4% magnesium alloy group was the slowest. (2) magnesium alloys with different zinc content could significantly inhibit the proliferation of human osteosarcoma U2OS cells, which were positively correlated with zinc content, and the toxicity of magnesium alloys with different zinc content to MC3T3-E1 cells was between 0 and 1 grade, and the toxicity of magnesium alloys with different zinc content to MC3T3-E1 cells was between 0 and 1, and the toxicity of magnesium alloys with different zinc content to MC3T3-E1 cells was between 0 and 1. (3) magnesium alloys with different zinc content could significantly promote the apoptosis of human osteosarcoma U2OS cells, which was positively correlated with zinc content. (4) magnesium alloy with different zinc content could up-regulate the expression of p53, down-regulate the expression of Bcl-2 protein and change the proportion of Bcl-2/Bax in human osteosarcoma U2OS cells, among which 4% magnesium alloy had the most significant effect. The results showed that zinc magnesium alloy could inhibit the proliferation and induce apoptosis of human osteosarcoma U2OS cells in vitro.
【作者单位】：煤炭总医院骨科;
【分类号】：R318.08;R738.1

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