电纺丝纳米纤维材料负载凝血酶制备新型止血敷料的可行性研究
发布时间：2019-05-24 23:37
【摘要】：任何一种临床常用的止血材料均存在其独特的性能优势，同时也有各自的局限。探索并研制性能更加优良的新型快速止血材料是很有必要的。电纺丝技术近年来已成为制备药物递送系统的一种有效方法。将速效止血药负载到电纺丝纤维材料上是一种很有吸引力的制备快速止血材料的途径。本研究拟探讨将速效止血药凝血酶负载固定到电纺纤维膜的表面制备高效快速止血材料的可行性及有效性。 目的: 探讨以电纺丝纳米纤维膜负载凝血酶制备新型止血敷料的可行性。 方法: 1、筛选合适的高分子材料，经电纺丝纳米纤维负载凝血酶制备试验性止血材料； 2、分别测试试验止血材料在不同温度、酸碱度及光照条件下的稳定性。 3、在不同的动物出血模型中与明胶海绵及可吸收止血绫对比，评估受试材料的止血性能。 结果: 以电纺丝膜负载凝血酶制备止血材料具有可行性。材料在低温条件下酶活更稳定，酸碱环境及光照条件下易失活。在肝脏渗血模型中试验材料的止血效果优于明胶海绵。在动脉搏动性出血模型中其止血可靠性优于可吸收止血绫。在大动脉缝合修补后渗血模型中试验材料的止血效果不及可吸收止血绫。 结论: 电纺丝膜负载凝血酶制备的新型止血材料有着良好的临床应用前景。
[Abstract]:Any kind of hemostatic material commonly used in clinic has its unique performance advantages, but also has its own limitations. It is necessary to explore and develop a new type of rapid hemostatic material with better properties. Electrospinning technology has become an effective method to prepare drug delivery system in recent years. Loading quick hemostatic drugs on electrospun fiber materials is an attractive way to prepare rapid hemostatic materials. The purpose of this study was to investigate the feasibility and effectiveness of immobilization of available hemostatic drug thrombin on the surface of electrospun fiber membrane to prepare efficient and rapid hemostatic materials. Objective: to investigate the feasibility of preparing a new hemostatic dressing with thrombin loaded with electrospun nanofiber membrane. Methods: 1. The suitable polymer materials were selected and the experimental hemostatic materials were prepared by electrospun nanofiber loaded with thrombin. 2. The stability of the experimental hemostatic materials under different temperature, pH and light conditions was tested respectively. 3. The hemostatic properties of the tested materials were evaluated by comparing with gelatin sponge and absorbable hemostatic silk in different animal bleeding models. Results: it is feasible to prepare hemostatic material by electrospinning membrane loaded thrombin. The enzyme activity of the material was more stable at low temperature, and it was easy to be inactivated in acid-base environment and light. The hemostatic effect of the experimental material is better than that of gelatin sponge in the model of liver bleeding. The hemostatic reliability of arterial pulsatile hemorrhage model is better than that of absorbable hemostatic model. The hemostatic effect of the experimental material in the bleeding model after artery suture repair was not as good as that of absorbable hemostasis. Conclusion: the new hemostatic material prepared by electrospinning membrane loaded with thrombin has a good clinical application prospect.
【学位授予单位】：中国人民解放军医学院
【学位级别】：博士
【学位授予年份】：2013
【分类号】：R318.08
本文编号：2485287
[Abstract]:Any kind of hemostatic material commonly used in clinic has its unique performance advantages, but also has its own limitations. It is necessary to explore and develop a new type of rapid hemostatic material with better properties. Electrospinning technology has become an effective method to prepare drug delivery system in recent years. Loading quick hemostatic drugs on electrospun fiber materials is an attractive way to prepare rapid hemostatic materials. The purpose of this study was to investigate the feasibility and effectiveness of immobilization of available hemostatic drug thrombin on the surface of electrospun fiber membrane to prepare efficient and rapid hemostatic materials. Objective: to investigate the feasibility of preparing a new hemostatic dressing with thrombin loaded with electrospun nanofiber membrane. Methods: 1. The suitable polymer materials were selected and the experimental hemostatic materials were prepared by electrospun nanofiber loaded with thrombin. 2. The stability of the experimental hemostatic materials under different temperature, pH and light conditions was tested respectively. 3. The hemostatic properties of the tested materials were evaluated by comparing with gelatin sponge and absorbable hemostatic silk in different animal bleeding models. Results: it is feasible to prepare hemostatic material by electrospinning membrane loaded thrombin. The enzyme activity of the material was more stable at low temperature, and it was easy to be inactivated in acid-base environment and light. The hemostatic effect of the experimental material is better than that of gelatin sponge in the model of liver bleeding. The hemostatic reliability of arterial pulsatile hemorrhage model is better than that of absorbable hemostatic model. The hemostatic effect of the experimental material in the bleeding model after artery suture repair was not as good as that of absorbable hemostasis. Conclusion: the new hemostatic material prepared by electrospinning membrane loaded with thrombin has a good clinical application prospect.
【学位授予单位】：中国人民解放军医学院
【学位级别】：博士
【学位授予年份】：2013
【分类号】：R318.08
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