利用二氧化钛静电纺丝和透明质酸甲基丙烯酸酯构筑用于细胞培养的纤维凝胶结构

发布时间：2019-05-28 06:15

【摘要】：通过观察生物体内骨的电镜图片,发现骨表面具有丰富的纤维微纳结构,在此观测的启发下,本实验设计将二氧化钛表面生物活性化的同时构筑这种纤维微纳结构,模仿骨表面。考虑到二氧化钛具有光催化性,且具有生物无毒性,本实验采用"自上而下"的一步法,在紫外光照的条件下,将二氧化钛静电纺丝与透明质酸衍生物在光照条件下通过双键稳定地连接起来。傅立叶-红外光谱分析表明实验成功地在二氧化钛静电纺丝表面嫁接上透明质酸分子。通过荧光和扫描电镜分析表明,间充质干细胞可以很好地在二氧化钛静电纺丝和透明质酸的复合结构上生长。这种构筑复合结构表面的方法,生物活化了二氧化钛纺丝的表面,同时模仿骨表面获得了微纳纤维拓扑结构。此外,可以将二氧化钛静电纺丝纺在不同种类的材料表面,从而在不同材料表面简便地得到可用于细胞培养的纤维表面结构,对于未来可实际应用的移植材料研发具有很好的借鉴意义。
[Abstract]:By observing the electron microscopic pictures of bone in organisms, it was found that there were abundant fiber microstructures on the bone surface. Inspired by this observation, the biological activation of titanium dioxide surface was designed to construct this fiber microstructure at the same time. Mimic the surface of the bone. Considering that titanium dioxide is photocatalytic and biotoxic, a "top-down" one-step method is used in this experiment under the condition of ultraviolet light. Titanium dioxide electrospinning and hyaluronic acid derivatives were stably connected by double bonds under light conditions. Fourier transform infrared spectroscopy (FTIR) analysis showed that hyaluronic acid molecules were successfully grafting on titanium dioxide electrospinning surface. Fluorescence and scanning electron microscope analysis showed that mesenchymal stem cells could grow well on the composite structure of titanium dioxide electrospinning and hyaluronic acid. The surface of titanium dioxide spinning was biologically activated by this method, and the topological structure of micro-nanofibers was obtained by imitating the surface of bone. In addition, titanium dioxide electrospinning can be spun on the surface of different kinds of materials, so that the surface structure of the fiber which can be used for cell culture can be easily obtained on the surface of different materials. It has a good reference significance for the research and development of transplanting materials which can be applied in the future.
【作者单位】：吉林大学化学学院超分子结构与材料国家重点实验室;
【分类号】：R318.08;TQ340.1

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