不同载银量载银羟基磷灰石细胞相容性与成骨活性的对比研究

发布时间：2019-05-29 17:51

【摘要】：目的·制备不同载银量的载银羟基磷灰石(Ag-HAp),探讨不同载银量Ag-HAp的细胞相容性及其对体外培养大鼠骨髓间充质干细胞(BMSC)成骨活性的影响,确定适宜载银量。方法·制备不同载银量(x_(Ag)值)的Ag-HAp(x_(Ag)0.3、x_(Ag)0.5、x_(Ag)0.7)并对其进行表征。将材料分为对照组(x_(Ag)0)和实验组(x_(Ag)0.3、x_(Ag)0.5、x_(Ag)0.7),检测各组材料对BMSC的细胞相容性、成骨相关基因的表达和碱性磷酸酶分泌情况的影响。结果·材料表征结果证实合成产物为Ag-HAp,最终载银量x_(Ag)0.7x_(Ag)0.5x_(Ag)0.3。细胞相容性实验结果显示:x_(Ag)0.3-HAp和x_(Ag)0.5-HAp为合格,x_(Ag)0.7-HAp有轻微毒性。Real-time PCR结果显示:与对照组比较,x_(Ag)0.3组和x_(Ag)0.5组成骨相关基因表达上调,x_(Ag)0.7组成骨相关基因表达下调或无明显改变。碱性磷酸酶定性定量实验显示:x_(Ag)0.3组和x_(Ag)0.5组碱性磷酸酶活性均高于对照组,x_(Ag)0.7组碱性磷酸酶活性低于对照组。结论·改变原材料中银的加入量(即改变x_(Ag)值)可制得不同载银量Ag-HAp,当x_(Ag)为0.3、0.5时,其细胞毒性实验结果为合格,且可促进大鼠BMSC成骨相关基因的表达及碱性磷酸酶的分泌。
[Abstract]:Objective to prepare silver-loaded hydroxyapatite (Ag-HAp) with different silver loading, to investigate the cellular compatibility of different silver-loaded Ag-HAp and its effect on (BMSC) osteogenic activity of rat bone marrow mesenchymal stem cells cultured in vitro, and to determine the suitable silver loading. Methods Ag-HAp (x _ (Ag) 0.3, x _ (Ag) 0.5, x _ (Ag) 0.7) with different silver loading (x _ (Ag) value) was prepared and characterized. The materials were divided into control group (x _ (Ag) 0) and experimental group (x _ (Ag) 0.3, x _ (Ag) 0.5, x _ (Ag) 0.7). The cellular compatibility of each group to BMSC was detected. Effect of osteogenic related gene expression and alkaline phosphatase secretion. Results the results of material characterization confirmed that the synthesized product was the final silver carrying capacity of Ag-HAp, 0.7x( Ag) 0.5x( Ag) 0.3. The results of cell compatibility test showed that x _ (Ag) 0.3-HAp and x _ (Ag) 0.5-HAp were qualified and x _ (Ag) 0.7-HAp was slightly toxic. Real-time PCR results showed that compared with the control group, The expression of bone-related genes in x( Ag) group was up-regulated, while the expression of bone-related genes in x( Ag) group was down-regulated or had no significant change. The results of qualitative and quantitative experiments showed that the alkaline phosphatase activity in x( Ag) group and x( Ag) group was higher than that in control group, and the alkaline phosphatase activity in x( Ag) group was lower than that in control group. Conclusion different silver loading Ag-HAp, can be prepared by changing the amount of silver in raw materials (that is, changing the value of x _ (Ag). When the x _ (Ag) is 0.3,0.5, the cytotoxicity test results are qualified. It can promote the expression of osteogenic related genes and the secretion of alkaline phosphatase in rats with BMSC.
【作者单位】：上海交通大学医学院附属第九人民医院口腔修复科;
【基金】：上海市科学技术委员会项目(114119a3900,14411964200)~~
【分类号】：R318.08

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