支架、细胞、因子:组织工程血管化策略的研究与前景

发布时间：2019-06-05 16:16

【摘要】：背景:随着组织工程皮肤、骨、软骨、血管、肌瓣等组织的再生与修复方面已取得显著的进展,组织工程组织血管化的障碍问题引起了学者们的广泛关注。目的:总结血管形成的机制(血管生成与血管再生),血管化的策略(种子细胞,支架材料,生长因子)以及组织血管化的模型(体内与体外)等与组织血管化相关的研究,为组织工程血管化的基础研究提供理论依据。方法:计算机检索2000年1月至2016年4月Pub Med、Springerlink、Web of Science、Science Direct数据库相关文献,检索词"tissue engineering、vascularization、scaffolds、cell growth factors、vasculogenesis、angiogenesis";并检索中文期刊CNKI、维普、万方数据库相关文献,检索词为"组织工程、血管化、细胞生长因子、血管的再生、血管的生成"。55篇与组织血管化相关的同时反映其最新研究进展的文章被纳入。结果与结论:体外构建组织内微血管中运用的较多,将支架材料做成具有血管样结构的模型,再种植二维细胞,在生长因子的刺激下,二维细胞朝着血管样结构的形状增殖、迁移,进而形成微小的血管。目前,大多数在体外实验能很好的形成血管的细胞-支架复合物,都沿着移植于体内方向发展。体内血管化模型中还存在着很多问题,移植于体内的炎症反应是不可避免的,它直接影响细胞-支架复合物的功能发挥及在体内的存活问题,在组织的再生与康复中,血管化仍是组织工程中一大难题。
[Abstract]:Background: with the remarkable progress in the regeneration and repair of tissue engineering skin, bone, cartilage, blood vessel, muscle flap and so on, the problem of vascularization of tissue engineering tissue has attracted extensive attention of scholars. Objective: to summarize the mechanism of vascularization (angiogenesis and vascular regeneration), the strategy of vascularization (seed cells, scaffold materials, growth factors) and the model of tissue vascularization (in vivo and in vitro). It provides a theoretical basis for the basic research of vascularization of tissue engineering. Methods: the relevant literature of Pub Med,Springerlink,Web of Science,Science Direct database from January 2000 to April 2016 was searched with the key words of "tissue engineering,vascularization,scaffolds,cell growth factors,vasculogenesis,angiogenesis". The Chinese journal CNKI, Weipu and Wanfang database were searched with the key words of "tissue engineering, vascularization, cell growth factor, vascular regeneration. 55 articles related to tissue vascularization and reflecting the latest research progress were included. Results & conclusion: the scaffolds are widely used in the construction of microvessels in vitro. The scaffolds are made into models with vascular structure, and then two-dimensional cells are planted. Under the stimulation of growth factors, the two-dimensional cells proliferate towards the shape of vascular-like structures. Migrate, which in turn forms tiny blood vessels. At present, most of the cell-stent complexes, which can form blood vessels in vitro, develop along the direction of transplantation in vivo. There are still many problems in the vascularization model in vivo. The inflammatory response of transplantation in vivo is inevitable, which directly affects the function of cell-stent complex and its survival in vivo, and in the process of tissue regeneration and rehabilitation. Vascularization is still a difficult problem in tissue engineering.
【作者单位】：南华大学药物药理研究所湖南省分子靶标新药研究协同创新中心;
【分类号】：R318.08

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