人血管内皮细胞生长因子121基因修饰材料修复桡骨火器性骨缺损

发布时间：2019-06-13 07:46

【摘要】：背景:骨缺损是导致火器性股损伤骨不连的首要原因,火器性损伤相对比较特殊。自体骨移植和异体骨移植均存在较大弊端,难以满足基层医院需求,采用具有良好血管能力、骨能力的组织工程化骨修复火器性骨缺损将是一种理想的、可行的修复方法。目的:探讨人血管内皮细胞生长因子121基因修饰材料在兔桡骨火器性骨缺损修复中的应用。方法:将128只家兔随机分为手术致伤组和火器伤组,每组64只。火器伤组采用56式滑膛枪发射质量为0.25 g的钢珠,建立火器伤桡骨损伤模型;手术致伤组仅用手术方法造成兔桡骨1.2 cm损伤模型。采用人血管内皮细胞生长因子121基因修饰材料进行修复处理,取家兔相关组织细胞,获得骨髓基质干细胞进行培养,制备多孔支架材料,并将获得的材料分别植入手术致伤组和火器伤组家兔桡骨骨缺损部位,分析人血管内皮细胞生长因子121基因修饰材料在兔桡骨火器性骨缺损修复中的应用。结果与结论:与手术致伤组相比,修复8,12,16周后,火器伤组家兔骨缺损部位灰度比值、健侧与修复侧桡骨的抗压缩力学比值降低(P0.05),新生骨面积增多(P0.05);修复2,4周后,骨缺损修复处局部血流量显著升高(P0.05)。结果说明,与单纯手术致桡骨损伤相比,烧伤致桡骨损伤修复过程中由于局部出现了缺血缺氧,采用人血管内皮细胞生长因子121基因治疗效果更理想。人血管内皮细胞生长因子121能够对骨髓基质细胞进行修复,将其运用于火器烧伤中能够提高其合成及分泌成血管因子,提高其局部血流量、降低抗压缩力学比值,且新生骨面积较大。
[Abstract]:Background: bone defect is the primary cause of nonunion of firearm thigh injury, and firearm injury is relatively special. Autologous bone transplantation and allogenic bone transplantation have great disadvantages, so it is difficult to meet the needs of grass-roots hospitals. It will be an ideal and feasible method to repair firearm bone defects with tissue engineering bone with good vascular ability and bone ability. Objective: to investigate the application of human vascular endothelial growth factor 121 gene modification in the repair of firearm bone defect of rabbit radius. Methods: 128 rabbits were randomly divided into surgical injury group (n = 64) and firearm injury group (n = 64). In the firearm injury group, the radius injury model was established by firing 0.25g steel beads with 56 type slide gun, and the rabbit radius 1.2 cm injury model was made only by operation in the operation group. Human vascular endothelial growth factor 121 gene modified material was used for repair and treatment. Rabbit related tissues and cells were obtained and cultured to prepare porous scaffolds. The obtained materials were implanted into the radius defect of rabbits in surgical injury group and firearm injury group respectively. The application of human vascular endothelial growth factor 121 gene modification material in the repair of firearm bone defect of rabbit radius was analyzed. Results and conclusion: compared with the surgical injury group, after 16 weeks of repair, the gray ratio of bone defect site, the ratio of anti-compression mechanics between the healthy side and the repaired side of radius in the firearm injury group decreased (P 0.05), and the area of new bone increased (P 0.05), and the local blood flow in the bone defect repair site increased significantly after 4 weeks of repair (P 0.05). The results showed that compared with radius injury induced by operation alone, human vascular endothelial growth factor 121 gene therapy was more effective because of local ischemia and hypoxia in the process of repairing radius injury caused by burn. Human vascular endothelial growth factor 121 can repair bone marrow stroma cells. Its application in firearm burn can increase its synthesis and secretion of vascular factors, increase its local blood flow, reduce the ratio of anticompression mechanics, and the area of new bone is large.
【作者单位】：河北大学附属医院;解放军第三军医大学大坪医院野战外科研究所第6研究室创伤烧伤复合伤国家重点实验室;
【基金】：全军医学科学技术研究“十五”指令性课题(01L060) 河北省科学技术研究与发展计划项目(072761417)~~
【分类号】：R318.08

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