人食管上皮细胞在多孔丝素蛋白支架上体外培养构建组织工程化食管的实验研究

发布时间：2019-06-27 12:32

【摘要】：目的：研究食管上皮细胞在多孔丝素蛋白支架上的粘附和生长情况,观察人食管上皮细胞与丝素蛋白支架的生物相容性,探索以丝素蛋白支架为基质构建组织工程化食管的可行性。方法：体外分离、培养、扩增人正常食管上皮细胞,并以一定数量接种于丝素蛋白支架上,在体外持续培养,通过组织学及扫描电镜观察食管上皮细胞在体外的增殖与分化情况以及细胞与丝素蛋白支架的生物相容性。结果：分离培养的人食管上皮细胞呈铺路石样,体外可大量扩增,细胞角蛋白抗体免疫组织化学染色阳性,体外培养可见食管上皮细胞在支架上粘附、生长良好,持续培养仍可保持食管上皮特性。结论：酶消化法适合食管上皮细胞的分离,所得细胞成分较纯,丝素蛋白支架有良好的生物相容性和细胞粘附性,无细胞毒性,适于食管上皮细胞的粘附生长,可作为组织工程食管的支架材料。
[Abstract]:Aim: to study the adhesion and growth of esophageal epithelial cells on porous silk fibroin scaffolds, to observe the biocompatibility of human esophageal epithelial cells with silk fibroin scaffolds, and to explore the feasibility of constructing tissue engineering esophagus with silk fibroin scaffolds. Methods: human normal esophageal epithelial cells were isolated, cultured and expanded in vitro and inoculated on silk fibroin scaffolds. The proliferation and differentiation of esophageal epithelial cells in vitro and the biocompatibility of esophageal epithelial cells with silk fibroin scaffolds were observed by histology and scanning electron microscope. Results: the isolated and cultured human esophageal epithelial cells were paving stone, which could be expanded in vitro, and the cytokeratin antibody was positive. Esophageal epithelial cells adhered to the scaffold in vitro and grew well. Continuous culture could still maintain the characteristics of esophageal epithelial cells. Conclusion: enzyme digestion is suitable for the isolation of esophageal epithelial cells. The obtained cells are pure. Silk fibroin scaffolds have good biocompatibility, cell adhesion and no cytotoxicity. They are suitable for the adhesion and growth of esophageal epithelial cells and can be used as scaffolds for tissue engineering esophagus.
【学位授予单位】：复旦大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R318.08

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