钛内植物表面纳米银超疏水抗菌涂层的构建和评估
发布时间：2019-07-01 16:06
【摘要】：研究背景 细菌粘附于材料表面是内植物感染的始动环节。因此，在材料表面构建抗菌涂层阻断细菌粘附是预防内植物感染的方向性策略。超疏水表面能抑制细菌粘附但不具备杀菌效果。而银抗菌谱广、热稳定性好、生物安全性高，但常规方法制备载银涂层存在释放速度快，抗菌有效期短等不足。本研究拟将抗菌性能优异的银引入超疏水表面构建，在提高超疏水表面抗菌性能同时抑制载银突释，实现持久抗菌目的。 目的 1.探讨TiO_2纳米管作为纳米银载体释放规律及长期抗菌能力；2.探讨载银/TiO_2纳米管表面修饰超疏水及抑制细菌粘附-杀灭细菌能力；3.探讨超疏水修饰对载银突释的抑制作用及释放规律影响。 方法 1.电化学阳极氧化法制备TiO_2纳米管载体层,通过修饰氟硅烷(PETS)制备超疏水表面，扫描电镜（SEM）、X线衍射（XRD）、接触角仪（CA）等方法分析表面特征及浸润性，评估超疏水涂层对细菌粘附作用。 2.脉冲电沉积法制备载纳米银/TiO_2纳米管涂层，SEM、X射线能谱（XPS）等离子质谱仪（ICPMS）等评估表面特征及载银释放，并评估抗菌性能及生物相容性。 3.在载纳米银/TiO_2纳米管表面修饰PETS制备超疏水涂层，评估对细菌粘附的抑制作用及杀灭粘附细菌能力。通过ICPMS评估超疏水对载银突释抑制作用及对释放规律影响。通过动物试验，探讨纳米银超疏水涂层的抗菌效能。 结果 1.电化学阳极氧化法可制备出排列规则的TiO_2纳米管载体层，管径80-100nm，管长200-400nm。在其表面修饰PETS制备超疏水涂层，接触角达168.2°，能明显抑制细菌粘附。 2.脉冲电沉积法可将纳米级银（10-30nm）均匀分布TiO_2纳米管表面及管内。而且以5-300、10-300、20-300样品银颗粒大小及分布最为均匀，表面含银比例分别为4.08At%、1.23At%、0.2At%。 3.样品（5-300、10-300、20-300）银释放时间14d，前3天由于表面银突释作用释放量较大，其后呈平稳释放状态。载银样品周围均能形成明显抑菌圈。 4.载银样品（5-300、10-300、20-300）具有良好生物相容性，对成骨细胞增殖、分化及矿化能力未见明显影响，，而且血液相容性良好。 5.在5-300、10-300、20-300样品表面修饰PETS，其接触角分别达160.1°、161.6°、158.0°。载银超疏水表面能有效抑制细菌粘附并杀灭粘附细菌，对载银突释具有一定程度的抑制作用，使载银释放更加平稳。 6.动物实验表明，相对于TiO_2钛棒，载银钛棒、超疏水载银钛棒均能预防骨髓炎及术后感染。 结论 1.脉冲电沉积法可在TiO_2纳米管表面及管内均匀沉积纳米级单质银颗粒。 2.载纳米银/TiO_2纳米管涂层能够持久释放，体外抗菌效果较好，而且具有良好的生物相容性。 3.载银超疏水涂层具有抑制细菌粘附-表面抗菌双重功效。 4.超疏水修饰对载银突释具有一定程度的抑制作用，使载银释放更加平稳。 5.载银钛棒、载银超疏水钛棒均在一定程度预防内植物术后感染。
[Abstract]:Study Background The bacterial adhesion to the surface of the material is the starting ring of the internal plant infection. Section. Therefore, the construction of an antibacterial coating on the surface of the material to block bacterial adhesion is a directional measure to prevent the infection of the plant. The super-hydrophobic surface can inhibit the adhesion of bacteria, but does not have the effect of sterilization. the silver antibacterial spectrum is wide, the heat stability is good, the biological safety is high, the silver-carrying coating prepared by the conventional method has the advantages of high release speed, short antibacterial period, and the like In this study, it is proposed to introduce the silver with excellent antibacterial performance into the super-hydrophobic surface, to improve the antibacterial performance of the super-hydrophobic surface and to inhibit the release of the silver-loaded surface, so as to realize the long-lasting antibacterial effect. It's all right. Objective 1. To investigate the release and long-term resistance of TiO _ 2 nanotubes as nano-silver carriers Surface modification of silver-bearing/ TiO _ 2 nanotubes, superhydrophobic and inhibition of bacterial adhesion-killing of fine particles the effect of super-hydrophobic modification on the release of silver-loaded silver and its release to set a gauge Method 1. The preparation of the TiO _ 2 nanotube carrier layer by electrochemical anodic oxidation, the preparation of superhydrophobic surface by modified fluorosilane (PETS), scanning electron microscopy (SEM), X-ray diffraction (XRD), contact angle instrument (CA), etc. Analysis of surface features and wettability, and evaluation of super-hydrophobic The surface features of silver-loaded nano-silver/ TiO _ 2 nanotube coating, SEM, X-ray energy spectrum (XPS) plasma mass spectrometer (ICPMS) were prepared by the pulse electrodeposition method. Preparation of super-hydrophobic coating on the surface modified PETS of nano-silver/ TiO _ 2 nano-tube to evaluate the adhesion of bacteria The inhibitory effect and the ability to kill the adherent bacteria. The super-hydrophobic effect on the silver-bearing process was assessed by the ICPMS. The effect of the release and the effect on the release rule. nanometer Results 1. The electrochemical anodic oxidation method can be used to prepare the TiO2 nanotube carrier layer with regular arrangement, and the tube diameter is 80- 100 nm, the tube length is 200-400 nm. The super-hydrophobic coating and the contact angle are prepared on the surface modified PETS. up to 168.2 掳, the adhesion of bacteria can be obviously inhibited.2. The pulse electrodeposition method can transfer the nano-grade silver (10-30n m) the surface and the tube of the TiO2 nanotube are uniformly distributed, and the size and the distribution of the silver particles in the sample of 5-300,10-300 and 20-300 are the most uniform, the silver-containing ratio of the surface is 4.0, 8At%, 1.23 At%, 0.2 At%.3. Sample (5-300,10-300,20-300) Silver release time 14d, first 3 days due to Table The release amount of the surface silver burst is large, and then it is flat. 4. the silver-carrying sample (5-300,10-300,20-300) has good biocompatibility, 5-300,10-300,20-300 sample surface modified PETS, The antennae reach 160.1 掳, 161.6 掳 and 158.0 掳 respectively. The silver-bearing super-hydrophobic surface can effectively inhibit the adhesion of bacteria and kill the adherent bacteria. 6. The results of animal experiments show that relative to TiO _ 2, the release of silver is more stable. titanium rod Conclusion:1. The pulse of titanium-bearing titanium rod and super-hydrophobic silver-bearing titanium rod can prevent osteomyelitis and post-operative infection. The nano-level elemental silver particles can be uniformly deposited on the surface of the TiO _ 2 nano tube and the tube by the electrodeposition method, and the nano-silver/ TiO _ 2 is loaded. The nanotube coating can be released for a long time, the in vitro antibacterial effect is good, 3. The silver-bearing super-hydrophobic coating has the double work of inhibiting bacterial adhesion-surface antibacterial and 4. the super-hydrophobic modification has a certain inhibition effect on the release of the silver-loaded burst, so that the carrie
【学位授予单位】：中国人民解放军医学院
【学位级别】：博士
【学位授予年份】：2013
【分类号】：R318.08;TB383.1
本文编号：2508624
[Abstract]:Study Background The bacterial adhesion to the surface of the material is the starting ring of the internal plant infection. Section. Therefore, the construction of an antibacterial coating on the surface of the material to block bacterial adhesion is a directional measure to prevent the infection of the plant. The super-hydrophobic surface can inhibit the adhesion of bacteria, but does not have the effect of sterilization. the silver antibacterial spectrum is wide, the heat stability is good, the biological safety is high, the silver-carrying coating prepared by the conventional method has the advantages of high release speed, short antibacterial period, and the like In this study, it is proposed to introduce the silver with excellent antibacterial performance into the super-hydrophobic surface, to improve the antibacterial performance of the super-hydrophobic surface and to inhibit the release of the silver-loaded surface, so as to realize the long-lasting antibacterial effect. It's all right. Objective 1. To investigate the release and long-term resistance of TiO _ 2 nanotubes as nano-silver carriers Surface modification of silver-bearing/ TiO _ 2 nanotubes, superhydrophobic and inhibition of bacterial adhesion-killing of fine particles the effect of super-hydrophobic modification on the release of silver-loaded silver and its release to set a gauge Method 1. The preparation of the TiO _ 2 nanotube carrier layer by electrochemical anodic oxidation, the preparation of superhydrophobic surface by modified fluorosilane (PETS), scanning electron microscopy (SEM), X-ray diffraction (XRD), contact angle instrument (CA), etc. Analysis of surface features and wettability, and evaluation of super-hydrophobic The surface features of silver-loaded nano-silver/ TiO _ 2 nanotube coating, SEM, X-ray energy spectrum (XPS) plasma mass spectrometer (ICPMS) were prepared by the pulse electrodeposition method. Preparation of super-hydrophobic coating on the surface modified PETS of nano-silver/ TiO _ 2 nano-tube to evaluate the adhesion of bacteria The inhibitory effect and the ability to kill the adherent bacteria. The super-hydrophobic effect on the silver-bearing process was assessed by the ICPMS. The effect of the release and the effect on the release rule. nanometer Results 1. The electrochemical anodic oxidation method can be used to prepare the TiO2 nanotube carrier layer with regular arrangement, and the tube diameter is 80- 100 nm, the tube length is 200-400 nm. The super-hydrophobic coating and the contact angle are prepared on the surface modified PETS. up to 168.2 掳, the adhesion of bacteria can be obviously inhibited.2. The pulse electrodeposition method can transfer the nano-grade silver (10-30n m) the surface and the tube of the TiO2 nanotube are uniformly distributed, and the size and the distribution of the silver particles in the sample of 5-300,10-300 and 20-300 are the most uniform, the silver-containing ratio of the surface is 4.0, 8At%, 1.23 At%, 0.2 At%.3. Sample (5-300,10-300,20-300) Silver release time 14d, first 3 days due to Table The release amount of the surface silver burst is large, and then it is flat. 4. the silver-carrying sample (5-300,10-300,20-300) has good biocompatibility, 5-300,10-300,20-300 sample surface modified PETS, The antennae reach 160.1 掳, 161.6 掳 and 158.0 掳 respectively. The silver-bearing super-hydrophobic surface can effectively inhibit the adhesion of bacteria and kill the adherent bacteria. 6. The results of animal experiments show that relative to TiO _ 2, the release of silver is more stable. titanium rod Conclusion:1. The pulse of titanium-bearing titanium rod and super-hydrophobic silver-bearing titanium rod can prevent osteomyelitis and post-operative infection. The nano-level elemental silver particles can be uniformly deposited on the surface of the TiO _ 2 nano tube and the tube by the electrodeposition method, and the nano-silver/ TiO _ 2 is loaded. The nanotube coating can be released for a long time, the in vitro antibacterial effect is good, 3. The silver-bearing super-hydrophobic coating has the double work of inhibiting bacterial adhesion-surface antibacterial and 4. the super-hydrophobic modification has a certain inhibition effect on the release of the silver-loaded burst, so that the carrie
【学位授予单位】：中国人民解放军医学院
【学位级别】：博士
【学位授予年份】：2013
【分类号】：R318.08;TB383.1
【参考文献】
相关期刊论文 前1条
1 李田，严煦世，黄伟星;固定膜光催化氧化反应器深度净化自来水研究[J];中国给水排水;1996年03期
本文编号：2508624
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