纳米银生物腹壁修复材料的生物安全性评价

发布时间：2019-07-06 12:17

【摘要】：目的研究自制的纳米银猪小肠黏膜下层(nano-silver modified porcine small intestinal submucosa,NS-PSIS)生物腹壁修复材料的生物安全性。方法取新鲜猪小肠,采用机械处理(去除浆膜层、黏膜层及肌层)、化学SDS法脱细胞制备猪小肠黏膜下层生物材料(porcine small intestinal submucosa,PSIS),配制50μg/mL的单体纳米银(直径15nm)溶液,将制备好的PSIS置入单体纳米银溶液中,制备成NS-PSIS,通过热原试验、皮肤刺激试验、皮内刺激试验、急性全身毒性试验及体内银吸收代谢试验对自制的纳米银生物腹壁修复材料进行生物安全性评价。结果纳米银生物腹壁修复材料无热原、皮肤刺激、皮内刺激及急性全身毒性。火焰原子吸收分光光度法显示纳米银生物腹壁修复材料不造成血液及肝、脑、肾等重要器官的银沉积。结论纳米银生物腹壁修复材料具有良好的生物安全性。
[Abstract]:Objective to study the biosafety of self-made nano-silver pig small intestinal submucosa (nano-silver modified porcine small intestinal submucosa,NS-PSIS) biological abdominal wall repair material. Methods the fresh pig small intestine was mechanically treated (removal of serosa, mucous membrane and muscle layer), the submucous biomaterial (porcine small intestinal submucosa,PSIS of pig small intestine was prepared by chemical SDS method, and the monomer silver nanoparticles (diameter 15nm) solution of 50 渭 g / mL was prepared. The prepared PSIS was put into the monomer silver nanoparticles solution, and the NS-PSIS, was prepared by pyrogen test, skin irritation test and intradermic stimulation test. Acute systemic toxicity test and in vivo silver absorption and metabolism test were used to evaluate the biosafety of self-made nano-silver biological abdominal wall repair materials. Results there were no pyrogen, skin irritation, intradermic irritation and acute systemic toxicity of nano-silver biological abdominal wall repair materials. Flame atomic absorption spectrophotometry showed that nano-silver biological abdominal wall repair materials did not cause silver deposition in blood, liver, brain, kidney and other important organs. Conclusion Nano-silver biological abdominal wall repair material has good biosafety.
【作者单位】：第二军医大学长征医院普外一科;
【基金】：国家自然科学基金(31100681) 上海市科学与技术委员会纳米专项基金(11nm0504800) 上海市晨光计划(11CG42)~~
【分类号】：R318.08

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