人BMSCs分化过程中免疫能力的实验研究
发布时间：2019-07-21 10:54
【摘要】：目的研究人BMSCs在成骨、成软骨及成脂分化过程中的免疫原性及其对外周血单个核细胞(peripheral blood mononuclear cell,PBMC)增殖的抑制能力。方法取健康志愿者骨髓分离培养BMSCs,分别进行成骨、成软骨和成脂诱导分化7、14、21 d。通过流式细胞术检测未分化及分化过程中BMSCs的人类白细胞抗原(human leukocyte antigen,HLA)Ⅰ类及Ⅱ类分子表达水平的改变。分离培养健康志愿者PBMC,按10∶1比例将PBMC与BMSCs共培养5 d,通过流式细胞术检测未分化及分化过程中BMSCs对PBMC增殖的抑制能力变化。结果未分化BMSCs表达HLA-Ⅰ类分子,基本不表达HLA-Ⅱ类分子。成骨、成软骨分化过程中各时间点HLA-Ⅰ、Ⅱ类分子表达无明显改变(P0.05),且HLA-Ⅱ类分子表达水平均较低。成脂分化14、21 d HLA-Ⅰ类分子表达逐渐升高,与0、7 d比较差异有统计学意义(P0.05);成脂分化7、14、21 d HLA-Ⅱ类分子表达也逐渐出现并升高,均显著高于0 d(P0.05)。PBMC在无抗CD3、CD28微球刺激下无明显增殖,加入抗CD3、CD28微球后显著增殖,未分化BMSCs能显著抑制PBMC的增殖。成骨、成软骨分化过程中BMSCs抑制PBMC增殖能力无明显改变(P0.05)。成脂分化7、14、21 d,BMSCs抑制PBMC增殖能力逐渐减弱,各时间点间差异均有统计学意义(P0.05)。结论人BMSCs成骨、成软骨分化过程中仍具有低免疫原性和较强的免疫抑制能力,可作为同种异体组织工程修复和生物治疗的细胞;而成脂分化后免疫原性升高、免疫抑制能力降低,不适合作为同种异体组织工程修复和生物治疗的细胞。
[Abstract]:Objective to study the immunogenicity of human BMSCs in osteogenesis, cartilage formation and adipogenic differentiation and its inhibitory effect on the proliferation of peripheral blood mononuclear cells (peripheral blood mononuclear cell,PBMC). Methods BMSCs, was isolated and cultured from bone marrow of healthy volunteers for osteogenesis, cartilage formation and adipogenic differentiation for 7, 14 and 21 days, respectively. The expression of human leukocyte antigen (human leukocyte antigen,HLA (human leukocyte antigen,HLA) class I and class II in undifferentiated and differentiated BMSCs was detected by flow cytometry. PBMC, was co-cultured with BMSCs for 5 days in the proportion of 10:1. Flow cytometry was used to detect the inhibitory effect of BMSCs on the proliferation of PBMC during undifferentiation and differentiation. Results HLA- class I molecules were expressed in undifferentiated BMSCs, but HLA- class II molecules were not expressed in undifferentiated HLA-. There was no significant change in the expression of HLA- class 鈪，
本文编号：2517130
[Abstract]:Objective to study the immunogenicity of human BMSCs in osteogenesis, cartilage formation and adipogenic differentiation and its inhibitory effect on the proliferation of peripheral blood mononuclear cells (peripheral blood mononuclear cell,PBMC). Methods BMSCs, was isolated and cultured from bone marrow of healthy volunteers for osteogenesis, cartilage formation and adipogenic differentiation for 7, 14 and 21 days, respectively. The expression of human leukocyte antigen (human leukocyte antigen,HLA (human leukocyte antigen,HLA) class I and class II in undifferentiated and differentiated BMSCs was detected by flow cytometry. PBMC, was co-cultured with BMSCs for 5 days in the proportion of 10:1. Flow cytometry was used to detect the inhibitory effect of BMSCs on the proliferation of PBMC during undifferentiation and differentiation. Results HLA- class I molecules were expressed in undifferentiated BMSCs, but HLA- class II molecules were not expressed in undifferentiated HLA-. There was no significant change in the expression of HLA- class 鈪，
本文编号：2517130
本文链接：https://www.wllwen.com/yixuelunwen/swyx/2517130.html
