纳米壳聚糖骨形成蛋白水凝胶修复下颌骨缺损实验研究
发布时间：2019-07-31 13:39
【摘要】：目的:观察可注射性纳米壳聚糖骨形成蛋白水凝胶修复颌骨缺损的效果。方法:以纳米壳聚糖为载体,加入重组人骨形成蛋白-2制备成可注射性纳米壳聚糖骨形成蛋白水凝胶(NCS/BMP-2 Gel)。取9只SD大鼠,在双侧背部皮下分别注射200μl(100μg/ml)NCS/BMP-2 Gel及单纯纳米壳聚糖水凝胶(NCS Gel)。于术后10、20、30 d分别处死3只,取材进行大体及常规病理学观察;选取54只SD大鼠,随机均分为3组(n=18)制备下颌骨缺损模型,实验组骨缺损中注入NCS/BMP-2Gel、对照组注入NCS Gel、空白组不注射。术后3、6、9周各组处死6只大鼠。分离下颌骨,用钼靶扫描图像,Image Pro-Plus软件比较其颌骨缺损面积;常规病理观察颌骨成骨情况。结果:实验侧、对照侧均形成皮下结节,实验侧30 d结节内可见类骨样结构;钼靶扫描检查:植入后3、6、9周时,实验组剩余骨缺损面积均低于对照组及空白组(P0.05);组织学观察显示,实验组下颌骨成骨情况优于对照组及空白组。结论:NCS/BMP-2水凝胶有良好的生物相容性,能促进颌骨缺损修复。
[Abstract]:Objective: to observe the effect of injectable nano-chitosan bone morphogenetic protein hydrogel in the repair of jaw defects. Methods: injectable nano-chitosan bone morphogenetic protein hydrogel (NCS/BMP-2 Gel).) was prepared by adding recombinant human bone morphogenetic protein-2 with nano-chitosan as carrier. Nine SD rats were injected subcutaneously with 200 渭 l (100 渭 g / ml) NCS/BMP-2 Gel and pure nano-chitosan hydrogel (NCS Gel). Respectively. Three rats were killed at 10, 20 and 30 days after operation, and 54 SD rats were randomly divided into three groups (n 鈮，
本文编号：2521363
[Abstract]:Objective: to observe the effect of injectable nano-chitosan bone morphogenetic protein hydrogel in the repair of jaw defects. Methods: injectable nano-chitosan bone morphogenetic protein hydrogel (NCS/BMP-2 Gel).) was prepared by adding recombinant human bone morphogenetic protein-2 with nano-chitosan as carrier. Nine SD rats were injected subcutaneously with 200 渭 l (100 渭 g / ml) NCS/BMP-2 Gel and pure nano-chitosan hydrogel (NCS Gel). Respectively. Three rats were killed at 10, 20 and 30 days after operation, and 54 SD rats were randomly divided into three groups (n 鈮，
本文编号：2521363
本文链接：https://www.wllwen.com/yixuelunwen/swyx/2521363.html
