Effect of a Hybrid Micro/Nano-integrated Titanium Surface on

发布时间：2021-04-11 01:39

　　The objective of this study was to investigate the effect of a new combined micro/nanoscale implant surface feature on osteoblasts’ behaviors including cell morphology, adhesion, proliferation, differentiation, and mineralization in vitro. A new micro/nano-hybrid topography surface was fabricated on commercial pure titanium（Cp Ti） by a two-step sandblasted acid-etching and subsequent alkali-and heattreatment（SA-AH）. The conventional sandblasted/acid-etching（SA） treatment and alkali and heat（AH） ... 

【文章来源】：Journal of Wuhan University of Technology（Materials Science）. 2017,32(02)EISCI

【文章页数】：10 页

【文章目录】：
1 Introduction
2 Experimental
    2.1 Sample preparation
    2.2 Surface characteristic by scanning electron microscopy
    2.3 Cell preparation and culture
    2.4 Rat osteoblasts attachment assay
    2.5 Immunofluorescence of cellular cytos-keletal arrangements
    2.6 Cell morphology observation
    2.7 Cell proliferations assay
    2.8 Real time quantitative polymerase chain reaction（q RT-PCR）assay
    2.9 Mineralization assay
    2.1 0 Statistical analysis
3 Results
    3.1 Surface characteristics of Ti discs
    3.2 Osteoblast adhesion assay
    3.3 Immunofluorescence of cellular cytoskeletal arrangements
    3.4 Cell proliferations assay
    3.5 Cell morphological observation
    3.6 Gene expression of osteoblast markers
    3.7 Mineralization assay
4 Discussion
5 Conclusions


【参考文献】：
期刊论文
[1]Effects of nanotopography on stem cell phenotypes[J]. Rajeswari Ravichandran,Clarisse CH Ng,Casey K Chan,Michael Raghunath,Seeram Ramakrishna.  World Journal of Stem Cells. 2009(01)



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