联合抑制缺氧诱导因子1α和STAT3对人喉鳞状细胞癌裸鼠移植瘤化疗增敏作用的研究

发布时间：2018-01-04 09:27

  本文关键词：联合抑制缺氧诱导因子1α和STAT3对人喉鳞状细胞癌裸鼠移植瘤化疗增敏作用的研究　出处：《临床耳鼻咽喉头颈外科杂志》2016年04期 　论文类型：期刊论文

  更多相关文章： 喉肿瘤 癌 鳞状细胞 信号转导子和转录激活子 缺氧诱导因子α 化疗

【摘要】：目的:探讨联合抑制缺氧诱导因子1α(HIF-1α)和信号转导子和转录激活子3(STAT3)对裸鼠人喉鳞状细胞癌移植瘤化疗增敏的作用。方法:将制备的喉癌移植瘤裸鼠随机分为空白对照组(A组)、顺铂化疗组(B组)、顺铂化疗联合AG490组(C组)、顺铂化疗联合HIF-1α~(-/-)组(D组)、顺铂化疗联合AG490加HIF-1α~(-/-)组(E组)和HIF-1α~(-/-)组(F组)。给予腹腔注射顺铂3 mg/kg连续3d,然后间隔给予顺铂和10mg/kg AG490连续12d。应用免疫组织化学法检测Ki67和HIF-1α表达。应用Western blot法检测p-STAT3表达。结果:C组、D组与B组比较HIF-1α表达均有显著差异(t值分别为2.782、3.873,均P0.05);E组与C组、D组比较HIF-1α表达均有显著差异(t值分别为6.140、3.667,均P≤0.01)。C组与B组比较p-STAT3表达明显降低(t=17.840,P0.01);D组与B组比较p-STAT3表达无明显差异(t=0.038,P0.05);E组与C组、D组比较p-STAT3表达明显降低(均P0.01)。E组与B组比较抑瘤率有显著差异(t=5.509,P0.01);E组与C组、D组比较抑瘤率均有显著差异(t值分别为3.422、2.718,均P0.05)。E组与B组、D组比较Ki67指数有显著差异(t值分别为8.307、4.524,均P0.01);E组与C组比较Ki67指数有显著差异(t=3.736,P0.05)。结论:联合抑制HIF-1α表达和STAT3通路对于头颈鳞状细胞癌的增殖抑制作用明显较单独抑制一种通路强。
[Abstract]:Objective: to investigate the combined inhibition of hypoxia inducible factor-1 伪 (HIF-1 伪) and signal transducers and activators of transcription (3STAT3). Methods: the nude mice were randomly divided into control group (group A). Group B was treated with cisplatin, group C with cisplatin combined with AG490, group D with cisplatin combined with HIF-1 伪 -r-r). Cisplatin chemotherapy combined with AG490 plus HIF-1 伪 -r-r-group (group E) and HIF-1 group (group F) were given intraperitoneal injection of cisplatin for 3 mg/kg for 3 days. Cisplatin and 10mg / kg AG490 were given at intervals for 12 days. The expression of Ki67 and HIF-1 伪 was detected by immunohistochemical method. Western was used. The expression of p-STAT3 was detected by blot. The expression of HIF-1 伪 in group D was significantly different from that in group B (2.782 卤3.873, P 0.05). There was significant difference in the expression of HIF-1 伪 between group E and group C (6.140 卤3.667). The expression of p-STAT3 in group C was significantly lower than that in group B (P 鈮，

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