生物视膜抑制结膜纤维瘢痕形成的实验研究

发布时间：2018-01-06 01:23

本文关键词：生物视膜抑制结膜纤维瘢痕形成的实验研究　出处：《眼科》2016年03期 　论文类型：期刊论文

【摘要】：目的观察生物视膜对结膜纤维瘢痕形成的抑制作用。设计实验研究。研究对象新西兰大白兔39只。方法 39只大白兔随机分为三组,分别为生理盐水组、丝裂霉素C(MMC)组、实验组,每组13只。用8 mm环钻和维纳斯剪制备右眼球结膜缺损的结膜损伤模型,左眼均不做处理。生理盐水组伤口处放置10 mm的生理盐水棉片2分钟;MMC组伤口处放置10 mm的浓度为0.4 mg/ml的MMC棉片2分钟。实验组将直径10 mm、厚度2 mm的生物视膜放置在术区后复水,用无损伤10-0缝合线,将材料和伤口周围的球结膜间断缝合8针。术后7、14、28天观察评估穹窿收缩率、伤口愈合率;术后7、14、28天取眼球做切片,应用HE和MASSON染色,观察浸润细胞种类和胶原纤维的疏密程度与排列。主要指标穹窿收缩率、伤口愈合率、浸润细胞种类和胶原纤维的疏密程度与排列。结果 28天时生理盐水组、MMC组、实验组的穹窿收缩率分别为45.1%±1.2%、25.5%±2.7%、23.6%±2.0%,实验组的穹窿收缩率明显小于生理盐水组(P0.001),实验组和MMC组差异无统计学意义(P0.05)。28天时,实验组伤口愈合率为100%±0.0%,MMC组伤口愈合率为41.8%±2.0%,生理盐水组为100%±0.0%,实验组和生理盐水组之间差异无统计学意义(P0.05)。HE染色:术后28天时,生理盐水组有少量成纤维细胞,细胞周围亦可见致密排列的胶原纤维,实验组则与正常结膜组织相似,MMC组浸润细胞数亦减少。MASSON染色:术后28天时,生理盐水组胶原纤维致密排列,实验组胶原纤维疏松未排列,MMC组胶原纤维疏松未排列。结论生物视膜对结膜纤维组织增生具有抑制作用,可防止结膜下瘢痕形成,抑制穹窿收缩,并能促进伤口正常愈合,促使正常结膜下基质形成。
[Abstract]:Objective To observe the inhibitory effect of bio membrane formation depending on conjunctival scar. The experimental research design. The research object of 39 New Zealand white rabbits. Methods 39 rabbits were randomly divided into three groups, respectively, the normal saline group, mitomycin C (MMC) group, the experimental group, 13 rats in each group. 8 mm trephine and Venus the shear damage model for the preparation of the right eye conjunctiva conjunctival defect in the left eye, do not handle. The saline cotton saline group wound placed 10 mM concentration for 2 minutes; group MMC wound placed 10 mm for 0.4 mg/ml MMC cotton for 2 minutes. The experimental group will be 10 mm diameter and 2 mm thickness of biological the membrane is placed in operation area after water, with no damage of 10-0 suture, the surrounding materials and wound suture needle 8. Conjunctival fornix 7,14,28 days after operation to evaluate the shrinkage rate, wound healing rate; postoperative 7,14,28 days from eyeball slice, using HE and MASSON staining to observe the infiltration of fine The degree of density and arrangement of cell types and collagen fibers. The main indicators of fornix shrinkage, wound healing rate, density and arrangement of infiltrating cell type and collagen fibers. Results the normal saline group, 28 days group MMC, experimental group were 45.1% dome shrinkage + 1.2%, 25.5% + 2.7%, 23.6% + 2%. The experimental group of the fornix shrinkage rate is less than the saline group (P0.001), there was no significant difference between the experimental group and MMC group (P0.05).28 days, the experimental group wound healing rate was 100% + 0%, MMC group wound healing rate was 41.8% + 2%, 0% + 100% in the saline group, there was no significant difference between the experimental group and saline group (P0.05):.HE staining 28 days after transplantation, the saline group has a small amount of fibroblasts, cells are visible around the dense arranged collagen fibers, experimental group and normal conjunctival tissue similar to that of MMC group also decreased.MASSON staining of infiltrating cells: 28 days after the operation, the saline group of dense collagen fibers arranged in the experimental group of collagen fiber loose arrangement, loose arrangement of collagen fibers in MMC group. Conclusion the biological membrane as has an inhibitory effect on the proliferation of fibrous tissue can prevent conjunctival and subconjunctival scar formation, inhibition of fornix contraction, and can promote wound healing, promote the formation of normal conjunctival stroma.

【作者单位】：首都医科大学附属北京同仁医院国家药物临床试验机构;首都医科大学附属北京同仁医院北京同仁眼科中心北京市眼科研究所眼科学与视觉科学北京市重点实验室;
【基金】：“重大新药创制”国家科技重大专项资助项目(2011ZX09302-007-05)
【分类号】：R77
【正文快照】： 结膜是眼球最前面的防御组织,且大部分暴露于外界环境中,易受钝力、锐器、表浅异物、化学烧伤、辐射伤等而形成结膜损伤,结膜损伤愈合的最终结局是结膜瘢痕形成,结膜瘢痕的成因除上述因素造成的结膜损伤以外,自身免疫性疾病、感染、青光眼滤过术、斜视手术、翼状胬肉等均可导

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