慢性鼻窦炎患者金黄色葡萄球菌耐药性分析

发布时间：2018-01-20 16:28

本文关键词： 金黄色葡萄球菌耐药基因耐药性 K-B法　出处：《中国病原生物学杂志》2017年01期 　论文类型：期刊论文

【摘要】：目的了解慢性鼻窦炎患者分离的金黄色葡萄球菌耐药情况。方法收集慢性鼻窦炎患者标本,进行金黄色葡萄球菌培养及分离纯化,表征患者病灶部位及菌株,K-B法分析其耐药性,PCR检测耐药基因。结果金黄色葡萄球菌是引发慢性鼻窦炎患者发生感染的原因及主要病原菌。药敏试验显示金黄色葡萄球菌对阿米卡星、苯唑西林、四环素、红霉素、万古霉素、环丙沙星、替考拉宁、克林霉素、利福平、庆大霉素的耐药率、中介率及敏感率分别为14.94%、39.08%、37.93%、59.20%、0.00%、31.03%、0.00%、47.70%、17.82%、45.98%,10.92%、0.00%、5.17%、1.72%、0.00%、2.87%、0.00%、9.20%、0.00%、6.90%和74.14%、60.92%、56.90%、39.08%、100.00%、66.09%、100.00%、43.10%、82.18%、47.13%。PCR扩增ermA、ermC、qacA、ermB、aac(6')/aph(2')、mecA基因大小分别为421、572、629、359、220和162bp,且其基因检出率分别为21.26%、10.92%、16.09%、35.06%、29.89%、43.10%。结论金黄色葡萄球菌是引发慢性鼻窦炎患者感染的主要病原菌,药敏试验显示其对万古霉素和替考拉宁敏感。
[Abstract]:Objective to investigate the drug resistance of Staphylococcus aureus isolated from patients with chronic sinusitis. Drug resistance was analyzed by K-B method. Results Staphylococcus aureus was the cause of infection in patients with chronic sinusitis and the main pathogenic bacteria. Drug sensitivity test showed that staphylococcus aureus to amikacin and oxacillin. Tetracycline, erythromycin, vancomycin, ciprofloxacin, teicoplanin, clindamycin, rifampicin and gentamicin were resistant, mediating and sensitive rates were 14.94% and 39.08%, respectively. 37.93 59.20: 0.002: 31.03and about 0.002: 47.70 and about 17.82, 45.98 and 10.92%, up to 0.00%. 5.17 the part of 1.72 and the part of 2.870.000 are about 6.90% and 74.14% 60.92% and 56.90%, about 0.00% and 9.20%, about 6.90% and 74.14%, 60.92% and 56.90%, respectively. 39.08, 100.00000 and 66.09, including 43.100.10 and 82.18, and 47.13. PCR was used to amplify ermAmC. The gene size of QAC Aer mBU 6 / aphora 2 was 421,572,629,359,220 BP and 162bp, respectively, and the size of the gene was 162bp, and the size of the gene was 162bp, and the size of the gene was 162bp. The detection rate of its gene was 21.26% and 10.92%, and 16.09%, 35.06% and 29.89%, respectively. Conclusion Staphylococcus aureus is the main pathogen causing infection in patients with chronic sinusitis. Drug sensitivity test shows that Staphylococcus aureus is sensitive to vancomycin and teicoplanin.
【作者单位】：重庆市中医院耳鼻喉科;重庆医科大学神经病学实验室;
【分类号】：R765.41
【正文快照】： 慢性鼻窦炎在医院鼻病患者中的比例达半数以上,常由于反复发作化脓性急性鼻窦炎感染而导致患者病程较长,长达10年左右。葡萄球菌属常会引起化脓性感染,而临床上常以是否产生凝固酶分为凝固酶阳性葡萄球菌和凝固酶阴性葡萄球菌[1]。金黄色葡萄球菌为凝固酶阳性葡萄球菌,可分解

【参考文献】