盐酸丙美卡因滴眼液对兔眼角膜中央厚度及角膜上皮形态学的影响

发布时间：2018-01-23 20:11

本文关键词： 角膜中央厚度盐酸丙美卡因角膜上皮细胞活体共聚焦显微镜　出处：《南昌大学》2012年硕士论文　论文类型：学位论文

【摘要】：目的：观察0.5%盐酸丙美卡因（Proparacaine Hydrochloride，PH）滴眼液对兔眼角膜上皮层及角膜中央厚度（Central Corneal Thickness，CCT）变化的影响。方法：取新西兰大白兔20只，全部选择其右眼进行实验，3%戊巴比妥全麻稳定后，先后使用Pentacam三维眼前节分析诊断系统、NT-530P眼压测厚一体机、A型超声角膜测厚仪测量CCT并记录，取其均值作为基准角膜中央厚度。在兔眼结膜囊内滴入1滴0.5%PH滴眼液后1、3、6、9、12、15、18、21min分别使用以上三种方法测量其值并进行比较。同时在点0.5%PH前后各时间段采用角膜共聚焦显微镜（IVCM）观察角膜上皮层是否有形态学变化。结果：使用0.5%PH前Pentacam、NT-530P、A超所测基准兔眼CCT分别为350.50±16.40μm、352.95±17.39μm、330.65±17.08μm。点0.5%PH后Pentacam、NT-530P组所测CCT在1min时分别为362.00±16.74μm和366.05±16.33μm，差异有统计学意义(P＜0.05)，，3min时分别为354.80±17.79μm和354.85±16.57μm，无统计学差异(P＞0.05)，以后各时间段CCT均未见明显变化(P＞0.05)。A超组CCT在点0.5%PH前与点0.5%PH后各时间段相比，均未见明显变化(P＞0.05)。IVCM显示点0.5%PH前后角膜浅表上皮细胞有变化，呈现一过性轻微水肿特征，角膜其他各层结构未见明显变化。结论： Pentacam和NT-530P在评估角膜测厚方面具有较好的可重复性及稳定性；0.5%PH滴眼液对兔眼Pentacam和NT-530P测量在1min内有影响，对A超则影响不明显；兔眼在滴用0.5%PH滴眼液3分钟后使用Pentacam和NT-530P测量角膜厚度更为合理；0.5%PH滴眼液可引起兔眼角膜浅表上皮细胞层形态学改变，可在24小时内恢复。
[Abstract]:Objective: 0.5% proparacaine Hydrochloride was observed. The effect of PH2 eye drops on the changes of corneal epithelial layer and central corneal thickness (CCT) in rabbit corneal epithelial layer and central corneal thickness was studied. Methods: Twenty New Zealand white rabbits were selected to perform 3% pentobarbital anesthesia stabilization in their right eyes, and then Pentacam three-dimensional anterior segment analysis and diagnosis system was used successively. CCT was measured and recorded by NT-530P intraocular pressure measuring machine and ultrasonic keratometer. Taking the mean value as the reference corneal central thickness, the rabbit conjunctival sac was injected with 1 drop of 0.5PH eye drops. The above three methods were used to measure and compare the values in 21min. At the same time, the corneal confocal microscope (IVCM) was used at every time before and after 0.5 PH. To observe whether there were morphological changes in corneal epithelium. Results: The CCT of rabbit eyes measured by pentacammoid NT-530PU A before 0.5 PH was 350.50 卤16.40 渭 m. 352.95 卤17.39 渭 m, 330.65 卤17.08 渭 m. Point 0.5 PH. Pentacam. The CCT of NT-530P group was 362.00 卤16.74 渭 m and 366.05 卤16.33 渭 m at 1 min, respectively (P < 0.05). 3min were 354.80 卤17.79 渭 m and 354.85 卤16.57 渭 m, respectively (P > 0.05). There was no significant change in CCT in each time period after that (P > 0.05). The CCT in group A was compared with that after 0.5 PH before point 0.5 and after 0.5 PH. No significant changes were found in the corneal superficial epithelial cells before and after PH, showing a transient slight edema in the point 0.5 and before and after PH, P > 0.05. IVCM showed that there was a slight edema in the cornea. There was no obvious change in the structure of the other layers of cornea. Conclusion: Pentacam and NT-530P have good reproducibility and stability in evaluating corneal thickness. 0.5 PH eye drops had an effect on the measurement of Pentacam and NT-530P in rabbit eyes within 1 minute, but not on A ultrasound. It was more reasonable to use Pentacam and NT-530P to measure corneal thickness in rabbit eyes after 3 minutes of dropping 0.5 PH eye drops. 0.5 PH eye drops caused morphological changes in the superficial epithelium of rabbit cornea and recovered within 24 hours.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R772.2

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