GSTA1基因C-69T多态性与糖尿病视网膜病变的相关性研究

发布时间：2018-01-26 05:17

  本文关键词： 糖尿病视网膜病变 GSTA基因 基因多态性　出处：《基因组学与应用生物学》2017年08期 　论文类型：期刊论文

【摘要】：糖尿病视网膜病变是糖尿病最常见的微血管病变之一,是工作年龄成人致盲的主要原因。氧化应激是活性氧生成和清除不平衡的结果,在糖尿病视网膜病变的发生中起重要作用。GSTA1是重要的氧化应激相关的谷胱甘肽硫转移酶之一,目前暂没有其多态性与糖尿病视网膜病变发病之间的研究。本实验以546例2型糖尿病样本为研究对象,根据直接眼底镜的结果把病人分成糖尿病视网膜病变组和有糖尿病但没有视网膜病变组。采用构象差异凝胶电泳方法检测GSTA1基因C-69T多态性。GSTA1基因C-69T多态性的CC、CT和TT种基因型在DR组的频率分别为79.8%、17.7%和2.5%,在NDR组的频率分别为77.8%、20.6%和1.6%,两组比较差异无统计学意义(x~2=1.254,p=0.561)。本实验结果表明,GSTA1基因C-69T多态性与中国江西汉族糖尿病视网膜病变的发病没有关联。
[Abstract]:Diabetic retinopathy is one of the most common microvascular lesions in diabetes and is the main cause of blindness in adults of working age. Oxidative stress is the result of imbalance of reactive oxygen species production and clearance. GSTA1 plays an important role in the pathogenesis of diabetic retinopathy. GSTA1 is one of the important oxidative stress-related glutathione sulfur transferases. At present, there is no study on the relationship between the polymorphism and the pathogenesis of diabetic retinopathy. 546 patients with type 2 diabetes mellitus were studied in this study. Patients were divided into diabetic retinopathy group and non-diabetic retinopathy group according to the results of direct ophthalmoscopy. Conformational differential gel electrophoresis was used to detect C-69T polymorphism of GSTA1 gene. G. CC with C-69T polymorphism of STA1 gene. The frequencies of CT and TT genotypes in Dr group were 79.7% and 2.5%, respectively, and those in NDR group were 77.8% and 1.6%, respectively. There was no statistical difference between the two groups. There is no association between C-69T polymorphism of GSTA1 gene and diabetic retinopathy in Jiangxi Han nationality.
【作者单位】： 南昌大学第二附属医院;南昌大学基础医学院生物化学与分子生物学教研室;南昌大学第一附属医院;南昌大学附属眼科医院;南昌大学公共卫生学院;
【基金】：江西省教育厅科学技术研究基金项目(No.:GJJ13165)资助
【分类号】：R587.2;R774.1
【正文快照】： *通讯作者,zwf100@163.comAssociation Analysis of the Polymorphism of GSTA1 Gene C-69T andDiabetic RetinopathyDeng Yan1Zhu Weifeng2*Yin Xiaolong1Xu Jixiong3Yi Jinglin4Li Yue51 The Second Affiliated Hospital to Nanchang University,Nangchang,330006;2 Departm，

本文编号：1464781