宽频带白噪声暴露对巴马香猪和豚鼠听力损伤的影响

发布时间：2018-01-27 11:08

本文关键词： 白噪声巴马香猪豚鼠动物模型毛细胞　出处：《中华耳科学杂志》2017年01期 　论文类型：期刊论文

【摘要】：目的通过120dB SPL宽频带白噪声对巴马香猪和豚鼠各进行单次3小时的暴露,观察比较该种噪声对两种模型动物听力损伤的特点,找到更适用于研究噪声性听力损伤的动物模型。方法选取8只6月龄ABR听阈正常的巴马香猪,和8只5~6周龄ABR听阈正常的豚鼠,设置4个ABR测听记录点:噪声暴露前,噪声暴露后即刻(P0)、1天(P1)、7天(P7)。全部测听结束后,取材制作耳蜗标本,进行扫描电镜观察形态。结果通过统计学分析,豚鼠组在120dB SPL宽频带白噪声暴露后即刻的ABR阈值与暴露1天的ABR阈值具有明显差异,具有统计学意义,而巴马香猪组则无明显统计学差异。豚鼠组在噪声暴露前和噪声暴露后7天的ABR阈值无明显统计学差异,而巴马香猪组却有明显的统计学差异。相对于豚鼠,巴马香猪耳蜗标本扫描电镜提示了更多的纤毛异常。结论120d B SPL宽频带白噪声暴露会对巴马香猪和豚鼠造成一定程度的听力损伤。其中巴马香猪较豚鼠表现更为明显的听力损失,且毛细胞出现了更多的病理改变,由此本研究表明在120d B SPL宽频带白噪声单次暴露3小时的条件下,巴马香猪是一个更适于白噪声与听力损伤的研究的动物模型,从而可以更好地为噪声性耳聋提供动物模型。
[Abstract]:Objective to observe and compare the characteristics of hearing impairment of Bama pig and guinea pig by 120 dB SPL broadband white noise exposure for 3 hours each. Methods eight Bama pigs with normal ABR hearing threshold and eight guinea pigs with normal ABR hearing threshold were selected. Four ABR audiometric recording points were set up: before noise exposure, immediately after noise exposure, P0 / P1 + P7 + P7 + P7 + P7 + P7 was taken immediately after noise exposure. Cochlea specimens were made after all the audiometry were completed. Results after 120 dB SPL white noise exposure, the ABR threshold of guinea pig group was significantly different from that of 1 day exposure. There was no significant difference in ABR threshold before noise exposure and 7 days after noise exposure in the guinea pig group. The Bama Xiang pig group, however, had a significant statistical difference compared to the guinea pigs. The scanning electron microscope of Bama pig cochlea specimen indicates more ciliated abnormalities. Conclusion 120d B. SPL broadband white noise exposure will cause a certain degree of hearing loss in Bama pigs and guinea pigs, in which Bama pigs have more obvious hearing loss than guinea pigs. There were more pathological changes in the hair cells, so this study showed that under the condition of 120 dB SPL wide band white noise exposure for 3 hours. Bama pig is a more suitable animal model for the study of white noise and hearing loss, which can provide a better animal model for noise-induced deafness.
【作者单位】：解放军总医院耳鼻咽喉头颈外科解放军耳鼻咽喉研究所聋病教育部重点实验室;福建医科大学附属第一医院耳鼻咽喉-头颈外科福建省耳鼻咽喉研究所;
【基金】：国家863青年科学家项目(2014AA020510) 国家973计划重大科学研究计划干细胞项目(2012CB967900) 中国科协创新驱动助力工程(2016CXQD01)~~
【分类号】：R764.433
【正文快照】： 噪声性耳聋(noise-induced deafness),亦可称之为噪声性听力损伤(noise-induced hearing loss),作为对影响现当代社会人类健康的一项疾病[1],其主要诱因为长期暴露于损害性噪声环境中,导致以内耳毛细胞损伤为主要表征的进行性感音神经性耳聋[2-4]。随着近年来科学技术的迅速发

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