血管内皮生长因子靶向抗体对大鼠角膜碱烧伤后新生血管抑制作用的研究

发布时间：2018-01-30 05:23

本文关键词： 血管内皮生长因子 MIL 角膜新生血管碱烧伤　出处：《眼科新进展》2017年11期 　论文类型：期刊论文

【摘要】：目的通过对角膜碱烧伤大鼠模型结膜下注射VEGF靶向抗体MIL60,观察其对角膜新生血管(corneal neovascularization,CoNV)生长的影响,初步探讨MIL60抑制大鼠CoNV生长的基本作用机制。方法建立碱烧伤诱导的SD大鼠CoNV模型(右眼造模),根据碱烧伤后第1天结膜下注射的药物不同将72只大鼠随机分为:25 mg·m L~(-1) MIL60组、地塞米松组、MIL60溶剂组和NaCl组。观察并记录CoNV生长情况及角膜的形态变化,通过软件分析Co NV的长度及累及面积。碱烧伤后第3天、第7天、第14天、第21天和第28天分别处死动物,取角膜组织行HE染色和免疫组织化学染色,同时检测角膜中血管内皮生长因子(vascular endothelial growth factor,VEGF)、VEGF受体-1(VEGF receptor-1,VEGFR-1)、VEGFR-2和基质金属蛋白酶-9(matrix metallopeptidase-9,MMP-9)蛋白的表达。结果在各个时间点,与MIL60溶剂组和NaCl组比较,地塞米松组、25 mg·m L~(-1) MIL60组CoNV面积和长度显著减少,差异均具有统计学意义(均为P0.01);25 mg·m L~(-1)MIL60组的CoNV长度和面积与地塞米松组相当(均为P0.05)。同时,MIL60还能有效降低角膜组织中VEGF、VEGFR-1、VEGFR-2以及MMP-9蛋白的表达。结论 MIL60能显著抑制角膜碱烧伤后CoNV的生长,并且还能减轻碱烧伤引起的炎症反应。
[Abstract]:Objective to conjunctival corneal alkali burn rat model by injection of VEGF antibody targeting MIL60, observe the corneal neovascularization (corneal neovascularization, CoNV) on the growth, study the basic mechanism of MIL60 inhibiting the growth of rat CoNV. Methods CoNV SD rats model induced by alkali burn up (right eye model) according to the first day after alkali burn, the subconjunctival injection of different drugs 72 rats were randomly divided into: 25 mg - M L~ (-1) MIL60 group, dexamethasone group, MIL60 solvent group and NaCl group. Observe and record the growth of CoNV and corneal morphological change through the analysis software of Co NV and the length of in the area. Third days after alkali burn for seventh days, fourteenth days, twenty-first days and twenty-eighth days respectively were animal corneas, HE staining and immunohistochemical staining, and detection of corneal vascular endothelial growth factor (vascular endothelial, growth factor, VEGF) VEGF, -1 (VEGF receptor-1, VEGFR-1 receptor, VEGFR-2) and matrix metalloproteinase -9 (matrix metallopeptidase-9 MMP-9) protein expression. Results at each time point, compared with MIL60 solvent group and NaCl group, dexamethasone group, 25 mg - M L~ (-1) MIL60 group of CoNV area and length were significantly decreased, the difference were statistically significant (P0.01); 25 mg m L~ (-1) MIL60 group CoNV and dexamethasone group equal length and area (P0.05). At the same time, MIL60 can effectively reduce the VEGF, VEGFR-1 and VEGFR-2 in corneal tissue, the expression of MMP-9 protein. Conclusion MIL60 can significantly inhibit the growth of CoNV after the corneal alkali burn, and can reduce inflammation caused by alkali burns.

【作者单位】：武警总医院眼科;火箭军总医院眼科;军事医学科学院;解放军总医院眼科;
【基金】：武警总医院院内课题(编号:wz2015011)~~
【分类号】：R779.13
【正文快照】： 血管内皮生长因子(vascular endothelial growth factor,VEGF)是目前已知的促新生血管形成相关因子中最主要的因子之一,它在新生血管发生和发展过程中都发挥着重要作用。研究发现,VEGF在角膜新生血管(corneal neovascularization,Co NV)的形成过程中也发挥重要作用[1]。在碱烧

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