姜黄素对人脉络膜黑色素瘤细胞株OCM-1血管生成拟态形态学和病理改变的影响

发布时间：2018-02-03 01:17

本文关键词： 脉络膜黑色素瘤姜黄素血管生成拟态　出处：《眼科新进展》2017年01期 　论文类型：期刊论文

【摘要】：目的研究不同浓度姜黄素对人脉络膜黑色素瘤细胞株(OCM-1)血管生成拟态形态学和病理学改变的影响。方法三维培养OCM-1细胞株,观察梯度浓度为0μmol·L~(-1)、10μmol·L~(-1)、20μmol·L~(-1)、30μmol·L~(-1)、40μmol·L~(-1)的姜黄素对OCM-1细胞株血管生成拟态形成的影响,分别进行形态学观察和病理学PAS染色。结果形态学观察显示:姜黄素0μmol·L~(-1)组:OCM-1细胞有浸润,在鼠尾胶上叠加生长,随着时间的延长,部分细胞逐渐伸展、变形,呈长条状、梭状或弧状;培养1周后细胞与细胞之间相互连接,并可呈管状、腔样、树枝样、网状等管腔结构,即血管生成拟态;姜黄素10μmol·L~(-1)组:部分OCM-1细胞能够形成条梭状和长条形,血管生成拟态形成;姜黄素20μmol·L~(-1)组:部分OCM-1细胞能呈长条形和梭形,但不能形成血管生成拟态;姜黄素30μmol·L~(-1)组:大部分细胞呈圆形,少数细胞能形成长条形和梭形,但不能形成血管生成拟态;姜黄素40μmol·L~(-1)组细胞皱缩成圆形,不能形成血管生成拟态。病理学PAS染色显示:姜黄素0μmol·L~(-1)组:大部分肿瘤细胞表达PAS阳性物质,相互连接形成环状、管腔样结构,即血管生成拟态;姜黄素10μmol·L~(-1)组:部分OCM-1细胞呈条梭状和长条形,PAS染色阳性,形成网格状血管生成拟态结构;姜黄素20μmol·L~(-1)组:部分OCM-1细胞呈长条形和梭形,PAS染色阳性,少部分呈网格状结构;姜黄素30μmol·L~(-1):大部分细胞呈圆形,PAS染色阴性,少数细胞能形成长条形和梭形,PAS染色阳性,但不形成网格状结构;姜黄素40μmol·L~(-1)组:细胞皱缩成圆形,PAS染色阴性,不形成网格状结构。结论姜黄素在体外可以抑制OCM-1细胞株血管生成拟态的形成,存在形态学和病理学改变,呈浓度依赖性。姜黄素浓度达到30μmol·L~(-1)时,对OCM-1细胞株血管生成拟态的形成具有明显的抑制作用。
[Abstract]:Objective to study the effects of curcumin at different concentrations on angiogenesis of human choroidal melanoma cell line OCM-1. Methods OCM-1 cell line was cultured in three dimensional culture. The gradient concentration was 0 渭 mol 路L ~ (-1), 10 渭 mol 路L ~ (-1) and 20 渭 mol 路L ~ (-1), 30 渭 mol 路L ~ (-1). The effect of curcumin (40 渭 mol 路L ~ (-1)) on angiogenesis of OCM-1 cell line was studied. Morphological observation and pathological PAS staining were performed. Results Morphologic observation showed that curcumin 0 渭 mol 路L ~ (-1) group had infiltrated cell lines and proliferated on rat tail glue. With the extension of time, some cells gradually extended and deformed, showing long stripe, fusiform or arc shape. After 1 week of culture, the cells were connected with each other, and could be tubular, cavernous, dendriform, reticular and other lumen structures, that is, angiogenic mimicry. Curcumin (10 渭 mol 路L ~ (-1)) group: some OCM-1 cells could form fusiform and long stripe, and angiogenesis mimicry; Curcumin 20 渭 mol 路L ~ (-1) group: some of OCM-1 cells could be long and fusiform, but could not form angiogenic mimicry. Curcumin 30 渭 mol 路L ~ (-1) group: most of the cells were round, a few cells could form long and fusiform, but could not form angiogenic mimicry. The cells of curcumin 40 渭 mol 路L ~ (-1) group shrank into a circle. The pathological PAS staining showed that curcumin 0 渭 mol 路L ~ (-1) group: most tumor cells expressed PAS positive substances and connected each other to form a ring. Lumen like structure, that is, angiogenesis mimicry; In curcumin 10 渭 mol 路L ~ (-1) group, some of the OCM-1 cells were positive for pas staining and formed mesh-like vasculature. In curcumin 20 渭 mol 路L ~ (-1) group, some of the OCM-1 cells showed long and fusiform pas staining, and a few of them were reticular. Curcumin 30 渭 mol 路L ~ (-1): most cells were negative for pas staining, and a few cells were positive for long and fusiform pas, but did not form reticular structure. Curcumin 40 渭 mol 路L ~ (-1) group: cells shrank to round pas staining negative. Conclusion curcumin can inhibit the angiogenesis of OCM-1 cell line in vitro and has morphological and pathological changes. When the concentration of curcumin reached 30 渭 mol 路L ~ (-1), it could inhibit the angiogenesis of OCM-1 cell line.
【作者单位】：天津中医药大学第一附属医院眼科;天津医科大学眼科医院;
【分类号】：R739.7
【正文快照】： 脉络膜黑色素瘤是成人眼内最常见的原发性恶性肿瘤,是一种严重危害人类健康的疾病[1]。肿瘤是血管生成依赖性的,迄今为止,已经发现并报道的肿瘤血液供应方式有内皮依赖性血管、血管生成拟态、马赛克血管3种[2]。血管生成拟态是近年来提出的一种全新的肿瘤内血管生成模式,即

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