兔眼角膜后弹力层撕除角膜内皮移植术后排斥反应的研究

发布时间：2018-02-08 11:35

本文关键词： 角膜内皮细胞角膜移植排斥反应　出处：《眼科新进展》2017年12期 　论文类型：期刊论文

【摘要】：目的通过高危兔眼模型分析角膜后弹力层撕除角膜内皮移植术后效果及免疫排斥反应特点。方法根据手术方法的不同将28只兔分成2组:穿透性角膜移植组(PK组)和角膜后弹力层撕除角膜内皮移植组(DSEK组),每组各14只,右眼为手术眼。术后用裂隙灯检查角膜混浊、水肿和新生血管情况,并记录排斥指数(rejection index,RI)和新生血管指数(neovascularization index,NI)。利用HE染色进行病理分析。采用实时荧光定量PCR技术测定各组白细胞介素-2(interleukin-2,IL-2)、IL-10和血管内皮生长因子(vas-cular endothelial growth factor,VEGF)mR NA的表达。结果眼前节检查显示PK组大部分兔眼角膜水肿、混浊及大量新生血管,而DSEK组只有少量兔眼出现角膜混浊水肿。PK组13眼(92.9%)出现排斥反应,明显高于DSEK组的4眼(28.6%),差异有统计学意义(P0.05)。病理组织切片显示,PK组单核细胞浸润明显高于DSEK组。实时荧光定量PCR检测结果显示,PK组IL-2、IL-10和VEGF mR NA的表达均明显高于DSEK组。结论 DSEK术后出现免疫排斥反应几率低,且比PK术后排斥反应程度弱,角膜内皮移植术是治疗角膜内皮疾病的一个理想选择。
[Abstract]:Objective to analyze the effect of corneal posterior elastic layer avulsion after corneal endothelium transplantation and the characteristics of immune rejection in high-risk rabbit eyes. Methods 28 rabbits were divided into two groups according to the different surgical methods: penetrating keratoplasty group and PK group. ) and DSEK group (14 rats in each group). The right eye was operated on. Corneal opacity, edema and neovascularization were examined by slit lamp after operation. The rejection index (RI) and neovascularization index (neovascularization) were recorded. The expression of interleukin-2interleukin-2IL-2mR10 and vascular endothelial growth factor (VEGF) were measured by real-time fluorescence quantitative PCR. The corneal edema of most of the rabbits in the competition group was revealed by the examination of the anterior segment of the fruit. Opacity and neovascularization, while in DSEK group, only a few rabbit eyes had corneal opacity edema. In PK group, 13 eyes (92.9 eyes) showed rejection. The expression of IL-2IL-10 and VEGF mRNA in the PK group was significantly higher than that in the DSEK group, and the expression of IL-2 IL-10 and VEGF mRNA in the PK group was significantly higher than that in the DSEK group. Conclusion the incidence of immune rejection after DSEK is low. Corneal endothelial transplantation is an ideal choice for the treatment of corneal endothelial diseases.
【作者单位】：厦门大学附属厦门眼科中心;福州眼科医院;
【基金】：福建省自然科学基金青年创新项目(编号:2012D056) 厦门市科技局社会发展项目(编号:3502Z20144042)~~
【分类号】：R779.65

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