水杨酸钠通过DA受体影响耳蜗螺旋神经节神经元NMDA受体与GABAa受体亚单位的表达

发布时间：2018-02-11 06:00

  本文关键词： 水杨酸钠 耳蜗螺旋神经节神经元 多巴胺受体 N-甲基-D-天冬氨酸受体 A型γ-氨基丁酸受体　出处：《临床耳鼻咽喉头颈外科杂志》2017年20期 　论文类型：期刊论文

【摘要】：目的:观察水杨酸钠(SS)对大鼠耳蜗螺旋神经节神经元(SGN)的多巴胺受体(DR)亚型表达的影响,以及观察SS作用下,DR对SGN的N-甲基-D-天冬氨酸(NMDA)受体和γ-氨基丁酸(GABA)a受体亚单位的影响,探讨SS介导的受体之间的相互作用。方法:应用免疫荧光技术,检测新生SD大鼠SGN中DR两家族DR1、DR2的表达;应用RT-PCR技术,检测SS处理后的SGN中DR1和DR2亚型的mRNA变化,观察在激活和抑制DR活动时SS对SGN的NMDA受体亚单位NR1、GABAa受体亚单位GABRα2的mRNA表达的影响。结果:免疫荧光显示:在SGN的胞体与突起均有DR1、DR2的免疫荧光显示。RT-PCR结果显示:(1)SGN有DR亚型(DRd1~DRd5)、GABRα2、NR1的表达;(2)5mmol/L SS作用SGN 30min后,除DRd3外,其余DR亚型及GABRα2、NR1的mRNA表达量均明显升高,其中DRd1表达增加34.64%(t=-5.123,P=0.007);DRd2表达增加34.60%(t=-5.206,P=0.006);DRd4表达增加20.87%(t=-3.337,P=0.029);DRd5表达增加26.42%(t=-6.054,P=0.004);GABRα2表达增加30.41%(t=-2.839,P=0.047);NR1表达增加39.22%(t=-6.243,P=0.003);(3)SS分别与多巴胺(100μmol/L)、DR1激动剂(SKF38393,20μmol/L)、DR2激动剂(Quinpirole,20μmol/L)分别作用SGN 30min后,GABRα2、NR1的mRNA表达量均明显升高,其中,GABRα2表达分别增加21.78%、27.45%、33.02%、33.42%(F=12.399,P=0.001),NR1表达分别增加28.70%、26.82%、29.03%、35.05%(F=50.395,P=0.000);(4)SS+DR1拮抗剂(SCH23390,20μmol/L)、SS+DR2拮抗剂(Eticlopride,20μmol/L)分别作用SGN 30min后,与单独SS作用相比,GABRα2的mRNA表达分别减少29.56%、37.10%(F=22.101,P=0.000),NR1的mRNA表达分别减少37.62%、32.83%(F=72.933,P=0.000)。结论:SS可诱导SGN的大部分DR亚型的mRNA表达明显升高;SS可能通过DR影响SGN上GABAaR、NMDAR的mRNA表达。
[Abstract]:Aim: to observe the effect of sodium salicylate (SSSS) on the expression of dopamine receptor (DRN) subtype in rat cochlear spiral ganglion neurons (SGNN). The effects of SS-induced Dr on the NMDA-NMDA-receptor and GABA receptor subunit of SGN were observed, and the interaction between the receptors mediated by SS-mediated was investigated. Methods: immunofluorescence technique was used. The expression of DR1DR2 in SGN of newborn SD rats was detected, and the mRNA changes of DR1 and DR2 subtypes in SGN treated with SS were detected by RT-PCR technique. To observe the effect of SS on the mRNA expression of SGN NMDA receptor subunit NR1GABAa receptor subunit GABR 伪 2 during activation and inhibition of Dr activity. Results: immunofluorescence showed that DR1 DR2 was present in the cell bodies and processes of SGN. RT-PCR showed that. The expression of GABR 伪 2NR1 in the SGN of Dr subtype DRd1, DRd5, and the expression of GABR 伪 2NR1 in SGN were detected after 30 minutes of SGN treatment with 5 mmol / L SS. With the exception of DRd3, all the Dr subtypes and the mRNA expression of GABR 伪 2nr 1 were significantly increased. The expression of DRd1 was increased by 34.64t-5.123P0. 007DRd2 and the expression of DRd4 was increased by 34.60t-5.206P0. 006DRd4. The expression of DRd5 was increased by 20.87t- 3.337P0. 029Dd5. The expression of GABR 伪 2 was increased by 26.42t-6.054P0.004 P0. 007. The expression of NR1 was increased by 39.22t-6.243P0. 243P0. 003SS and the expression of F3938320DR2 agonist Quinle20 / L SGN was significantly increased after the expression of NR1 was significantly increased, and the expression of NR1 was increased by 39.22t-6.243P0. 003SS, respectively. The expression of GABR 伪 2 was increased by 21.78 + 27.45 and 33.022, respectively, and the expression of NR1 was increased by 28.70,26.82and 29.035.050.395P0.0004SS DR1 antagonist, Eticlopride20 渭 mol / L, 20 渭 mol / L, respectively, after 30 minutes of treatment with Eticlopride20 渭 mol / L, a DR2 antagonist, Eticlopridea, a 20 渭 mol / L DR2 antagonist of Sch 23390,20 渭 mol / L, respectively. Compared with SS alone, the mRNA expression of GABR 伪 2 was reduced by 29.56% and 37.10%, respectively. The mRNA expression of NR1 was decreased by 37.62%, 32.833%, respectively. Conclusion the mRNA expression of most Dr subtypes of SGN can be induced by VSS to increase the mRNA expression of GABARARN NMDAR on SGN by the influence of VSS on the mRNA expression of GABARARN NMDAR on SGN. [WT5 "HZ] [WT5" BZ] [WT5 "BZ] [WT5" BZ] [WT5 "BZ] [WT5" BZ] [WT5BZ] [WT5BZ]
【作者单位】： 广西医科大学第一附属医院耳鼻咽喉头颈外科;钦州市第二人民医院耳鼻咽喉头颈外科;
【基金】：国家自然科学基金(No:81560174,81360157) 广西自然科学基金(No:2014GXNSFAA118137) 2017广西研究生教育创新计划项目(No:YCSW2017103)
【分类号】：R764

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