沉默TRIM27基因对鼻咽癌5-8F细胞增殖、侵袭与迁移的影响

发布时间：2018-02-21 23:50

本文关键词： TRIM蛋白鼻咽癌细胞增殖细胞侵袭细胞迁移　出处：《中国病理生理杂志》2017年01期 　论文类型：期刊论文

【摘要】：目的:检测TRIM27蛋白在鼻咽癌组织和鼻咽部正常组织中的表达水平,观察沉默TRIM27对鼻咽癌5-8F细胞增殖、侵袭和迁移能力的影响。方法:通过免疫组化技术检测鼻咽癌组织和鼻咽部正常组织中TRIM27的表达水平,采用实时荧光定量PCR(real-time PCR)和Western blot法分别检测5-8F细胞和NP69细胞中TRIM27的mRNA和蛋白表达水平;利用脂质体2000将TRIM27干扰序列转入5-8F细胞中;采用real-time PCR检测转染后细胞中TRIM27 mRNA表达水平的变化,Western blot法检测转染后细胞中TRIM27蛋白水平的变化;CCK-8法检测细胞活力的变化;细胞平板集落形成实验观察转染后细胞增殖能力的变化;Transwell实验检测细胞侵袭能力的变化;划痕实验观察细胞迁移能力的变化。结果:免疫组化结果显示TRIM27在鼻咽癌组织中的表达率高于鼻咽部正常组织;TRIM27基因沉默后5-8F细胞增殖、侵袭和迁移能力均明显受到抑制(P0.05)。结论:TRIM27在鼻咽癌5-8F细胞中可能充当癌基因的角色。沉默TRIM27基因可以明显抑制鼻咽癌细胞的增殖、侵袭和迁移能力。
[Abstract]:Objective: to detect the expression of TRIM27 protein in nasopharyngeal carcinoma (NPC) and nasopharyngeal normal tissues, and to observe the proliferation of 5-8F cells by silencing TRIM27. Methods: immunohistochemical technique was used to detect the expression of TRIM27 in nasopharyngeal carcinoma and normal nasopharyngeal tissues. The expression levels of mRNA and protein of TRIM27 in 5-8F cells and NP69 cells were detected by real-time fluorescence quantitative PCR(real-time PCR and Western blot, respectively, and TRIM27 interference sequence was transferred into 5-8F cells by liposome 2000. The changes of TRIM27 mRNA expression in transfected cells were detected by real-time PCR. The changes of TRIM27 protein levels in transfected cells were detected by Western blot assay and the changes of cell viability were detected by CCK-8 method. The changes of cell proliferation ability after transfection were observed by cell plate colony formation assay and the change of cell invasion ability was detected by Transwell assay. Results: immunohistochemical results showed that the expression rate of TRIM27 in nasopharyngeal carcinoma was higher than that in normal nasopharyngeal tissue after silencing TRIM27 gene. Conclusion the invasion and migration ability of human nasopharyngeal carcinoma (NPC) cell line 5-8F may be inhibited by TRIM27 gene silencing, and the ability of proliferation, invasion and migration of nasopharyngeal carcinoma cells can be significantly inhibited by silencing TRIM27 gene.
【作者单位】：温州医科大学第一临床学院;台州市立医院耳鼻咽喉科;
【分类号】：R739.63

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