不同免疫表型鼻息肉中黏膜炎症模式与组织重塑特征

发布时间：2018-02-25 02:23

本文关键词： 鼻息肉嗜酸粒细胞白细胞介素- 白细胞介素- 中性粒细胞 Th细胞亚群　出处：《临床耳鼻咽喉头颈外科杂志》2016年16期 　论文类型：期刊论文

【摘要】：目的:研究鼻息肉中免疫表型特点,比较分析TH2与TH17亚型鼻息肉的黏膜炎症模式及组织重塑特征。方法:鼻内镜手术中获取88例慢性鼻-鼻窦炎伴鼻息肉患者息肉组织,18例正常对照鼻中隔偏曲患者下鼻甲黏膜组织;ELISA法检测炎症及组织重塑相关免疫指标。免疫组织化学观察主导细胞因子蛋白表达及炎症细胞浸润状况等。结果:全部鼻息肉组织中TH效应细胞因子表达阳性IL-5占21%、IL-17占16%、IFN-γ占9%,而54%的息肉中无任何其他TH细胞关键性细胞因子的表达。其中IL-17表达阳性(TH17型)鼻息肉具有中性粒细胞炎症相关递质[主要是髓过氧化物酶(MPO),IL-1β,IL-6和IL-8]显著增高;组织学IL-17表达显著并MPO+细胞浸润明显;同时伴有组织重塑相关TGF-β1、MMP7等相对增高趋势。反之在IL-5阳性(TH2型)鼻息肉中以嗜酸性炎症相关递质升高为主(主要是IL-5,嗜酸性阳离子蛋白,TIgE和SAE-IgE);组织学IL-5表达显著并嗜酸粒细胞浸润特征;而TGF-β1与MMP7蛋白表达显著降低。结论:鼻息肉存在各种不同免疫表型并具有特殊炎症模式及组织重塑特点,即IL-5阳性鼻息肉具有嗜酸性炎症特征而组织重塑不显著;IL-17阳性息肉具有中性粒细胞炎症特征且具有组织重塑特征。
[Abstract]:Objective: to study the immunophenotypic characteristics of nasal polyps, The characteristics of mucosal inflammation and tissue remodeling of nasal polyps of TH2 and TH17 subtypes were compared and analyzed. Methods: 88 cases of chronic rhinosinusitis with nasal polyps were obtained from 88 cases of nasal polyps with nasal polyps. 18 cases of normal control patients with nasal septum deviation. Immunohistochemistry was used to observe the expression of dominant cytokines protein and infiltration of inflammatory cells. Results: th effector cells in all nasal polyps were detected by Elisa. The positive expression of IL-5 and IL-17 accounted for 9% of IFN- 纬, while no other cytokines of th cells were found in 54% polyps. The nasal polyps have neutrophil inflammation related transmitters (mainly medullary hypertrophy). The levels of IL-1 尾 IL-6 and IL-8 were significantly increased. The histological expression of IL-17 was significant and the infiltration of MPO cells was obvious. At the same time, there was a relative increase trend of tissue remodeling associated with TGF- 尾 1 and MMP7. On the contrary, in nasal polyps with IL-5 positive, the increase of eosinophilic related transmitters (mainly IL-5, eosinophilic cationic protein TIgE and SAE-IgE7) was predominant, and the expression of histological IL-5 was significant. And eosinophilic granulocyte infiltration; The expression of TGF- 尾 1 and MMP7 protein was significantly decreased. Conclusion: nasal polyps have different immunophenotypes and special inflammatory patterns and tissue remodeling characteristics. That is, IL-5 positive nasal polyps have eosinophilic inflammatory characteristics, while tissue remodeling is not significant. IL-17 positive polyps have the characteristics of neutrophil inflammation and tissue remodeling.
【作者单位】：西藏自治区人民医院耳鼻咽喉科;四川大学华西医院耳鼻咽喉头颈外科上呼吸道实验室;
【基金】：国家自然科学基金项目(No:81260158,81560172) 中国博士后科学基金项目(No:2014M552363)
【分类号】：R765

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