鼻咽癌组织中Cathepsin D和MMP-7表达的特点及意义

发布时间：2018-03-01 10:43

本文关键词： 鼻咽癌组织蛋白酶D 基质金属蛋白酶联合评估肿瘤侵袭肿瘤转移　出处：《广东医学》2017年05期 　论文类型：期刊论文

【摘要】：目的探讨鼻咽癌组织中组织蛋白酶D(Cathepsin D)和基质金属蛋白酶7(MMP-7)表达特点及其联合评估肿瘤侵袭转移的价值。方法采用免疫组化法检测42例鼻咽癌活检组织(A组)和30例同期慢性鼻咽炎活检组织(B组)中Cathepsin D和MMP-7蛋白表达情况。比较鼻咽癌组织Cathepsin D和MMP-7蛋白阳性表达与其阴性表达患者的肿瘤T分期、N分期、微血管密度(MVD)及淋巴管密度(LVD)等。采用Spearman无条件相关分析法分析鼻咽癌组织Cathepsin D蛋白阳性表达率和MMP-7蛋白阳性表达率与其T分期、N分期、MVD及LVD的关系。结果与B组比较,A组Cathepsin D和MMP-7蛋白阳性表达率均提高(P0.05)。A组Cathepsin D蛋白阳性表达+MMP-7蛋白阳性表达患者的T3~4分期患者比例、N_(2~3)患者比例均高于其Cathepsin D蛋白阴性表达+MMP-7蛋白阳性表达患者和Cathepsin D蛋白阴性表达+MMP-7蛋白阴性表达患者(P0.05)。A组Cathepsin D蛋白阳性表达+MMP-7蛋白阳性表达患者的MVD及LVD均高于其Cathepsin D蛋白阳性表达+MMP-7蛋白阴性表达患者、Cathepsin D蛋白阴性表达+MMP-7蛋白阳性表达患者和Cathepsin D蛋白阴性表达+MMP-7蛋白阴性表达患者(P0.05)。A组Cathepsin D蛋白阳性表达+MMP-7蛋白阴性表达患者和Cathepsin D蛋白阴性表达+MMP-7蛋白阳性表达患者的MVD及LVD亦均高于其Cathepsin D蛋白阴性表达+MMP-7蛋白阴性表达患者(P0.05)。Spearman无条件相关分析结果显示,鼻咽癌组织Cathepsin D蛋白阳性表达率和MMP-7蛋白阳性表达率与其T分期、N分期、MVD及LVD均呈正相关(r=0.798、0.785、0.855、0.842,P0.05)。结论鼻咽癌组织Cathepsin D和MMP-7蛋白阳性表达率高且均与其T分期、N分期、MVD及LVD相关,联合检测鼻咽癌组织Cathepsin D和MMP-7蛋白表达可作为鼻咽癌肿瘤侵袭转移的评估的参考指标。
[Abstract]:Objective to investigate the expression of cathepsin D in nasopharyngeal carcinoma (Cathepsin D) and matrix metalloproteinase 7 (MMP-7) expression and combined evaluation characteristics of tumor invasion and metastasis. Methods using immunohistochemistry in 42 cases of nasopharyngeal carcinoma biopsy (A group) and 30 cases of chronic nasopharyngitis biopsies (B group) in Cathepsin D and MMP-7 the expression of Cathepsin in nasopharyngeal carcinoma. The positive expression of D and MMP-7 protein and negative expression in patients with tumor T stage, N stage, microvessel density (MVD) and lymphatic vessel density (LVD). Using Spearman correlation analysis method to analyze the condition of nasopharyngeal carcinoma Cathepsin positive expression rate of D protein and MMP-7 protein positive expression rate with the T staging, N staging, the relationship between MVD and LVD. Results compared with B group, A group, Cathepsin D and MMP-7 protein expression rate was increased (P0.05) positive expression of.A Cathepsin D protein +MMP-7 protein positive group The expression of T3~4 in patients with stage N_ (2~3) the proportion of patients, the proportion of patients were higher than those of the Cathepsin negative expression of D protein positive expression of +MMP-7 protein and Cathepsin protein in patients with D negative expression of +MMP-7 protein in patients with negative expression of MVD (P0.05) and LVD.A group Cathepsin positive expression of D protein positive expression of +MMP-7 protein in patients with positive expression of Cathepsin was higher than that of the D +MMP-7 protein expression in patients with negative Cathepsin, negative expression of D protein positive expression of +MMP-7 protein and Cathepsin protein in patients with D negative expression of +MMP-7 protein in patients with negative expression of Cathepsin (P0.05).A group D protein positive expression of +MMP-7 protein in patients with negative expression of Cathepsin and D protein negative expression of MVD and LVD positive expression of +MMP-7 protein in patients were higher than those in the Cathepsin D protein negative expression of +MMP-7 protein in patients with negative expression (P0.05).Spearman unconditional correlation analysis showed that nasopharyngeal carcinoma group The Cathepsin positive expression rate of D protein and the expression rate of MMP-7 and T staging, N staging, MVD and LVD were positively correlated (r=0.798,0.785,0.855,0.842, P0.05). Conclusion Cathepsin in nasopharyngeal carcinoma D and MMP-7 protein positive expression rate is high and the T stage, N stage, MVD and LVD, the reference index of combined detection of nasopharyngeal carcinoma Cathepsin D and MMP-7 protein expression in nasopharyngeal carcinoma can be used as evaluation of tumor invasion and metastasis.

【作者单位】：南华大学附属第一医院耳鼻咽喉科;南华大学医学院病理学教研室;
【基金】：国家自然科学基金资助项目(编号:81372894)
【分类号】：R739.63

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