变应性鼻炎新西兰兔静脉血中CRLR和CGRP-RCP的表达及变化研究

发布时间：2018-03-06 08:54

本文选题：降钙素受体样受体　切入点：降钙素基因相关肽受体组分蛋白　出处：《重庆医学》2017年28期 　论文类型：期刊论文

【摘要】：目的观察新西兰兔变应性鼻炎模型静脉血中降钙素受体样受体(CRLR)、降钙素基因相关肽受体组分蛋白(CGRP-RCP)的表达及变化,分析其作用机制。方法 24只新西兰兔分为正常对照组(A组)和变应性鼻炎组(B组)。用卵清蛋白(OVA)致敏新西兰兔并行鼻腔激发刺激,制作变应性鼻炎模型。观察两组新西兰兔行为改变,并取其鼻甲及鼻中隔黏膜制作HE切片,观察新西兰兔鼻腔黏膜病理改变。收集静脉血,采用ELISA检测血清中CRLR、CGRP-RCP的水平变化。结果行为学评分显示:两组新西兰兔在鼻腔激发后第1~5天的行为学评分比较差异有统计学意义(P0.05)。HE染色显示:A组鼻腔黏膜及间质稍水肿血管轻度增生、充血,炎性细胞浸润不明显,少量鼻分泌物;B组间质内水肿明显,血管增生、充血,可见大量嗜酸性粒细胞和其他炎性细胞浸润。B组CRLR、CGRP-RCP水平较A组明显增高(t=5.143、10.595,P0.05)。结论CRLR、CGRP-RCP在变应性鼻炎的发病机制中可能起着重要作用。
[Abstract]:Objective to observe the expression and changes of calcitonin receptor like receptor (CRLRN) and calcitonin gene-related peptide receptor component (CGRP-RCPP) in the venous blood of New Zealand rabbit allergic rhinitis model. Methods 24 New Zealand rabbits were divided into normal control group (group A) and allergic rhinitis group (group B). Ovalbumin (OVA) was used to sensitize New Zealand rabbits with nasal stimulation. In order to make allergic rhinitis model, the behavior changes of New Zealand rabbits were observed, and the nasal turbinate and nasal septum mucosa were taken to make HE sections, and the pathological changes of nasal mucosa were observed, and venous blood was collected. Results Behavioral score showed that there was a significant difference in behavioral score between the two groups on the 1st day after nasal stimulation. He staining showed that the nasal mucosa and stroma of group A were significantly different. Slight edema, slight proliferation of blood vessels, Hyperemia, inflammatory cell infiltration was not obvious, a small amount of nasal secretion group B interstitial edema, vascular proliferation, hyperemia, It can be seen that the level of CGRP-RCP in group B is significantly higher than that in group A. conclusion the level of CGRP-RCP may play an important role in the pathogenesis of allergic rhinitis in group B.Conclusion the level of CGRP-RCP may play an important role in the pathogenesis of allergic rhinitis in group B.Conclusion the level of CGRP-RCP may play an important role in the pathogenesis of allergic rhinitis.
【作者单位】：桂林医学院附属医院耳鼻咽喉头颈外科/桂林医学院耳鼻咽喉科学研究所;
【基金】：基金项目:人口健康安全技术开发与应用研究(20130120-6)
【分类号】：R765.21

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