增生型糖尿病视网膜病变患眼玻璃体、增生膜组织miR-195表达及意义

发布时间：2018-03-09 03:34

  本文选题：增生型糖尿病视网膜病变　切入点：玻璃体　出处：《山东医药》2017年16期 　论文类型：期刊论文

【摘要】：目的探讨miR-195在增生型糖尿病视网膜病变(PDR)发生、发展中的作用及机制。方法选取PDR患者56例(观察组)、特发性黄斑裂孔患者30例(对照组),均为单侧发病。两组均接受常规玻璃体切割术,观察组术中采集玻璃体及视网膜前增生膜组织,对照组采集玻璃体及内界膜组织。采用实时荧光定量PCR法检测两组玻璃体和膜组织miR-195和高迁移率族蛋白B1(HMGB1)mRNA相对表达量,Western blotting法检测两组膜组织HMGB1蛋白相对表达量,并分析观察组玻璃体、膜组织中miR-195与HMGB1 mRNA相对表达量的关系。结果观察组玻璃体及膜组织miR-195相对表达量均低于对照组,HMGB1 mRNA相对表达量均高于对照组(P均0.01)。观察组膜组织HMGB1蛋白相对表达量为0.94±0.11,对照组为0.58±0.09,两组比较P0.05。观察组玻璃体及膜组织中miR-195与HMGB1 mRNA相对表达量均呈负相关(r分别为-0.369、-0.508,P均0.05)。结论 PDR患眼玻璃体及增生膜组织中miR-195表达均降低,其可能通过负性调控HMGB1表达而参与PDR的发生、发展。
[Abstract]:Objective to investigate the occurrence of miR-195 in proliferative diabetic retinopathy. Methods 56 patients with PDR (observation group) and 30 patients with idiopathic macular hole (control group) were selected. In the observation group, the vitreous body and the preretinal proliferative membrane were collected during the operation. The relative expression of miR-195 in vitreous and membranous tissue and high mobility group protein B1HMGB1 were detected by real-time fluorescence quantitative PCR method. The relative expression of HMGB1 protein in the two groups was detected by Western blotting. The vitreous body of the observation group was analyzed. Results the relative expression of miR-195 in vitreous and membrane tissues in the observation group was lower than that in the control group (P < 0.01). The relative expression of HMGB1 protein in the membrane tissue of the observation group was higher than that in the control group (P < 0.01). The relative expression of miR-195 and HMGB1 mRNA in vitreous and membranous tissues in the observation group was negatively correlated with that in the control group (-0.369 and -0.508) P respectively. Conclusion the expression of miR-195 in vitreous and proliferative membrane tissues of the patients with PDR was lower than that of the control group (P < 0.05). It may participate in the occurrence and development of PDR through negative regulation of HMGB1 expression.
【作者单位】： 平顶山市第二人民医院;河南省眼科研究所河南省立眼科医院;
【基金】：河南省科技发展计划项目(142102310429)
【分类号】：R587.2;R774.1
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本文编号：1586841