应用双向电泳和免疫印迹技术分析软叶针葵花粉变应原

发布时间：2018-03-09 17:40

本文选题：软叶针葵花粉　切入点：变应原　出处：《临床耳鼻咽喉头颈外科杂志》2016年11期 　论文类型：期刊论文

【摘要】：目的:采用双向电泳和免疫印迹技术对软叶针葵花粉提取液蛋白质组分进行分析,寻找软叶针葵花粉的主要变应原。方法:提取软叶针葵花粉变应原,应用双向电泳(2-DE)结合免疫印迹技术,获得相应的电泳图谱和免疫印迹图谱。结果:软叶针葵花粉蛋白提取液经双向电泳后,用考马斯亮蓝染色,得到的电泳图显示约有601个主要蛋白斑点。分子量(Mr)分布约20~130kD;等电点(pI)主要集中在4.0~8.0。免疫印迹分析结果显示,阳性组与对照组的差异蛋白点数约为19个。结论:双向电泳联合免疫印迹技术对软叶针葵花粉提取液蛋白质组分分析,可寻找到其主要变应原,并可进一步用于变应原致敏组分的研究。
[Abstract]:Objective: to find out the main allergens of sunflower powder extracted from soft leaf needle and sunflower powder by two dimensional electrophoresis and Western blotting. Methods: to extract the allergen of sunflower powder from soft leaf needle. The electrophoretic and immunoblotting patterns were obtained by two dimensional electrophoresis and Western blotting. Results: the protein extract of sunflower powder from soft leaf needle was stained with Coomassie brilliant blue after two dimensional electrophoresis. The electrophoretogram showed that there were 601 major protein spots, the molecular weight of Mr was about 20 ~ 130kD, and the isoelectric point (Pi) was mainly concentrated at 4.0 ~ 8.0. The results of Western blot analysis showed that, The difference of protein points between the positive group and the control group is about 19. Conclusion: Two-dimensional electrophoresis combined with Western blotting can be used to analyze the protein components of sunflower powder extract from soft leaf needle, and the main allergens can be found. It can be further used in the study of allergen allergens.
【作者单位】：海口市人民医院耳鼻咽喉头颈外科;海口市人民医院中心实验室;
【基金】：国家自然科学基金项目(No:81160129) 海南省自然科学基金资助项目(No:30871,811145) 海南省国际科技合作重点计划项目(No:2012-GH010) 海口市重点科技计划项目[No:海科立2007-0080号,海科工信立(2012)72号] 海南省卫生厅科研项目(No:琼卫2007-79)
【分类号】：R765.21

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