脉冲噪声暴露后大鼠下丘核生长相关蛋白-43表达的研究

发布时间：2018-03-10 01:23

本文选题：脉冲噪声　切入点：听性脑干反应　出处：《听力学及言语疾病杂志》2017年05期 　论文类型：期刊论文

【摘要】：目的探讨脉冲噪声暴露对大鼠下丘核生长相关蛋白-43(growth associated protein,Gap-43)表达的影响。方法 SPF级成年雄性SD大鼠30只,随机分为正常对照组6只、噪声暴露组24只,正常对照组不接受噪声暴露,噪声暴露组接受脉冲噪声暴露,噪声平均压力峰值156dB SPL,脉宽0.25ms,单次暴露6s,共暴露50次。于噪声暴露前及暴露后3、7、14、28d分别测试对照组及噪声暴露组(每个时间点6只)大鼠听性脑干反应(auditory brainstem response,ABR)阈值;然后取双侧下丘进行免疫组化染色,通过灰度值检测各组大鼠下丘Gap-43蛋白表达水平。结果噪声暴露后各组大鼠ABR反应阈显著高于正常组(P0.01),噪声暴露后14、28d组ABR阈值显著低于暴露后3d组(P0.05);噪声暴露后各组大鼠双侧下丘Gap-43蛋白表达显著高于正常组(P0.01),暴露后28d组大鼠下丘Gap-43蛋白表达显著低于暴露后3d组(P0.05)。结论脉冲噪声暴露可导致大鼠ABR反应阈显著升高,下丘Gap-43蛋白高表达;脉冲噪声暴露后不同时间大鼠ABR阈值升高和下丘Gap-43蛋白高表达的变化规律可能与听皮层突触重塑有关。
[Abstract]:Objective to investigate the effect of pulsed noise exposure on the expression of Gap-43 associated protein associated protein in the inferior colliculus nucleus of rats. Methods Thirty adult male SD rats of SPF grade were randomly divided into normal control group (n = 6) and noise exposure group (n = 24). The normal control group did not receive noise exposure, while the noise exposure group received impulse noise exposure. The mean noise pressure peak value was 156dB SPLL, pulse width 0.25ms, single exposure 6s, exposure 50 times. The auditory brainstem response (ABR) thresholds of the control group and the noise exposure group (6 rats at each time point) were measured before and after exposure to noise for 3 weeks and 14 days after exposure, and the threshold values of auditory brainstem response (ABR) were measured respectively in the control group and the noise exposure group (6 rats at each time point). Then the bilateral inferior colliculus was taken for immunohistochemical staining. Results the ABR response threshold of rats after noise exposure was significantly higher than that of normal group (P 0.01), and the threshold of ABR in group 14: 28 days after noise exposure was significantly lower than that in group 3 days after exposure to noise (P 0.05). The expression of Gap-43 protein in the bilateral inferior colliculus of the rats in the control group was significantly higher than that in the control group (P 0.01), and the expression of Gap-43 protein in the inferior colliculus of the rats 28 days after exposure was significantly lower than that in the group of 3 days after exposure. Conclusion Pulsed noise exposure can significantly increase the threshold of ABR response in rats. The high expression of Gap-43 protein in inferior colliculus and the increase of ABR threshold and Gap-43 protein expression in inferior colliculus of rats at different time after impulse noise exposure may be related to synaptic remodeling of auditory cortex.
【作者单位】：武汉大学人民医院耳鼻咽喉头颈外科;解放军武汉总医院耳鼻咽喉科;
【基金】：国家自然基金面上项目(30772397) 国家自然科学青年基金(81100712)资助
【分类号】：R764

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