变压器噪声暴露对豚鼠旷场行为及听功能的影响

发布时间：2018-03-12 06:34

本文选题：变压器噪声　切入点：动物行为学　出处：《听力学及言语疾病杂志》2016年01期 　论文类型：期刊论文

【摘要】：目的探讨变压器噪声对豚鼠旷场行为及听功能的影响。方法取32只健康成年(5~6月龄)豚鼠随机分为实验组和对照组,每组16只。实验组给予录制的变压器噪声(声压级范围40.8~55dB SPL,频谱范围150~2 000Hz)连续暴露28天,10小时/天(晚10点到早上8点),对照组在相同条件下饲养,无噪声暴露。在噪声暴露前1天、噪声暴露第28天记录实验组、对照组的旷场行为并对两组豚鼠进行DPOAE、ABR检测后,取耳蜗行常规耳蜗铺片和硝酸银染色,观察耳蜗毛细胞损害情况。结果噪声暴露28天后,两组豚鼠旷场行为差异无统计学意义(P0.05);1.0、2.0、3.0、4.0、6.0、8.0kHz DPOAE反应幅值差异无统计学意义(P0.05)。噪声暴露前1天及噪声暴露28天后实验组豚鼠的ABR反应阈分别为31.38±1.78、32.13±2.24dB SPL,与对照组(分别为31.89±1.03和31.75±1.57dB SPL)比较差异均无统计学意义(P0.05)。噪声暴露28天后实验组豚鼠耳蜗外毛细胞形态基本正常,毛细胞缺失率(1.31%±0.52%)与对照组(0.85%±0.23%)比较差异无统计学意义(P0.05)。结论声压级范围为40.8~55dB SPL、频谱范围为150~2 000 Hz的变压器噪声连续暴露28天(10小时/天)对成年豚鼠旷场行为及听功能无明显影响。
[Abstract]:Objective to investigate the effect of transformer noise on open field behavior and auditory function of guinea pigs. Methods 32 healthy adult guinea pigs were randomly divided into experimental group and control group. 16 rats in each group were exposed to recorded transformer noise (sound pressure level 40.85dB SPLand spectrum 1502000Hz) for 28 days / day (10:00 to 8am). The control group was fed under the same conditions. The open field behavior of experimental group and control group were recorded one day before noise exposure and 28 days after noise exposure. The cochlea of the two groups were detected by DPOAEN ABR. Cochlea cochlea were taken for routine cochlea spreading and silver nitrate staining. The damage of cochlear hair cells was observed. Results after 28 days of noise exposure, There was no significant difference in open field behavior between the two groups (P 0.05). There was no significant difference in the amplitude of DPOAE response between the two groups. The ABR response threshold of the experimental group was 31.38 卤1.732.13 卤2.24dB before noise exposure and 28 days after noise exposure (31.89 卤1.03, respectively). Compared with 31.75 卤1.57dB SPL1, there was no significant difference between them (P 0.05). After 28 days of noise exposure, the morphology of cochlear hair cells in the experimental group was basically normal. There was no significant difference between the loss rate of hair cells (1.31% 卤0.52%) and the control group (0.85% 卤0.23). Conclusion the sound pressure level range is 40.855 dB SPLand the frequency spectrum range is 150 ~ 2 000 Hz transformer noise exposure for 28 days / 10 hours / day) to adult guinea pig open field behavior and hearing. The function has no obvious effect.
【作者单位】：武汉大学人民医院耳鼻咽喉头颈外科;中国电力科学研究院;
【基金】：国家电网公司总部科技项目
【分类号】：R764.433

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