灵长类动物自发性年龄相关眼病动物模型的探讨

发布时间：2018-03-21 11:18

本文选题：灵长类眼病模型　切入点：糖尿病视网膜病变　出处：《首都医科大学学报》2017年01期 　论文类型：期刊论文

【摘要】：目的本研究筛查老年猴群中自然产生的白内障,年龄相关性黄斑变性,糖尿病视网膜病变等眼病,通过对比灵长类动物眼底疾病改变与人类眼底疾病改变,探讨在老年猴群中建立年龄相关性眼病实验动物模型的可行性。方法 39只食蟹猴,平均年龄(11.9±2.05)岁,最小9岁,最大17岁和13只猕猴,平均年龄(15.5±5.2)岁,最小7岁,最大24岁,进行空腹血糖和2 h糖耐量检测。应用裂隙灯、眼底相机、眼压计初步筛查眼病和眼压值,疑似病例应用频域光学相干断层扫描(spectral-domain optical coherence tomography,SD-OCT)对视网膜病变进行确诊。结果血糖筛查中发现4例食蟹猴,3例猕猴血糖升高,眼底未发现类似人类糖尿病视网膜病变的相关病理改变。13只食蟹猴和6只猕猴出现不同程度的晶状体混浊。食蟹猴眼压均值为右眼(15.98±3.315)mm Hg(1 mm Hg=0.133 k Pa),左眼(15.82±4.021)mm Hg;猕猴眼压右眼(14.9±3.867)mm Hg,左眼(14.7±3.329)mm Hg。1例食蟹猴出现高眼压,眼底有早期青光眼改变。眼底图像筛查中,发现2例食蟹猴眼底出现散在玻璃膜疣分布于视网膜周围部。1例食蟹猴单眼出现地图样脉络膜病变。结论在老年猴筛查中发现了自发形成的晶状体混浊、视网膜玻璃膜疣以及脉络膜萎缩等年龄相关性眼病,证实了灵长类动物与人类有着相似的病理生理改变。同时未见与人类相似的糖尿病视网膜病变眼底改变,可能由于灵长类动物因高血糖引起的微血管结构改变与人类不同,或糖尿病尚未产生视网膜病变,为年龄相关性眼病灵长类动物模型提供了依据。
[Abstract]:Objective to screen natural cataract, age-related macular degeneration, diabetic retinopathy and other eye diseases in the elderly monkeys, and to compare the changes of primate fundus diseases with those of human fundus diseases. To explore the feasibility of establishing an experimental animal model of age-related ophthalmopathy among the elderly monkeys, 39 crab eating monkeys with an average age of 11.9 卤2.05 years, a minimum of 9 years, a maximum of 17 years and 13 macaques, with an average age of 15.5 卤5.2 years, a minimum of 7 years, and a maximum age of 24 years, were used. Fasting blood glucose and 2 h glucose tolerance were measured. Slit lamp, fundus camera and IOP were used to screen IOP and IOP. Retinopathy was diagnosed by frequency-domain optical coherence tomography (optical) optical coherence tomographygraphy (SD-OCTT). Results in the blood glucose screening, 4 cases of macaque macaque were found to have elevated blood glucose, and 3 cases of macaque macaques were found to have elevated blood glucose. 13 macaques and 6 macaques had different degrees of lens opacity. The mean IOP of the macaques was 15.98 卤3.315 mm Hg(1 Hg=0.133 k Pag and 15.82 卤4.021 mm Hgof the left eye. The intraocular pressure was 14.9 卤3.867mm Hg in the right eye, 14.7 卤3.329mm in the left eye and 14.7 卤3.329mm in the left eye. There are early glaucoma changes in the fundus. It was found that 2 cases of macaque macaque were scattered in vitreous membrane verruca around the retina. 1 case of monocular choroidal lesion was found in 1 case. Conclusion spontaneous lens opacification was found in the screening of senile monkey. Age-related ophthalmopathy, such as vitreous verruca and choroidal atrophy, has proved that primates have similar pathophysiological changes to human beings. It is possible that the changes of microvascular structure caused by hyperglycemia in primates are different from those in humans, or that retinopathy has not been produced in diabetes mellitus, which provides evidence for age-related eye disease primate models.
【作者单位】：首都医科大学附属北京同仁医院北京同仁眼科中心北京市眼科研究所眼科学与视觉科学北京市重点实验室;
【分类号】：R77

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