角膜新生血管和淋巴管与房水中VEGF-A和VEGF-C表达量关系的临床研究

发布时间：2018-03-21 18:34

本文选题：角膜新生血管　切入点：角膜新生淋巴管　出处：《重庆医科大学》2012年硕士论文　论文类型：学位论文

【摘要】：目的：通过对因角膜病变行角膜移植患者的角膜组织中的角膜新生血管（Corneal neovascularization，CNV）和角膜新生淋巴管（Corneallymphangiogenesis，CL）行免疫组化染色计数以及检测其房水中血管内皮生长因子A（Vascular endothelial growth factor-A，VEGF-A）和血管内皮生长因子C（Vascular endothelial growth factor-C，VEGF-C）的表达的研究，建立房水中VEGF-A和VEGF-C与角膜新生血管和角膜新生淋巴管之间的定量关系，揭示房水中VEGF-A和VEGF-C表达量与角膜新生血管和角膜新生淋巴管数变化规律，，为角膜病患者实行角膜移植的手术时机选择提供理论依据。方法：10例因各类角膜溃疡和碱烧伤后需要行角膜移植的患者纳入本研究，根据病史记录病程长短，以及术前患者的裂隙灯照片，了解患者术前情况。采用管腔内皮非特异受体CD31，定量检测角膜新生血管数目（Blood vessels counting，BVC），采用淋巴管内皮细胞特异性抗体LYVE-1行免疫组织化学染色，定量检测角膜新生淋巴管的数目（Lymphatic vessels counting， LVC）。将10个实验组样本和10个对照组（单纯年龄相关性白内障患者）的房水用酶联免疫吸附测定法（Enzyme linked immunosorbentassay，ELISA）对比试验，测出房水中VEGF-A和VEGF-C的表达量。探索房水中VEGF-A和VEGF-C的表达量与BVC和LVC之间的关系。结果：免疫组化染色结果10例实验组角膜都有新生血管，4例既有新生血管又有新生淋巴管。实验组和对照组房水中VEGF-A和VEGF-C表达量有显著性差异，角膜新生血管、新生淋巴管与其房水的VEGF-A和VEGF-C的表达量有明显相关性。结论：角膜病变后有角膜新生血管和新生淋巴管的患者，其房水中的VEGF-A和VEGF-C的表达量明显增高，且VEGF-A和角膜新生血管的数量呈正相关，VEGF-C和角膜新生淋巴管归一化后变化趋势分析提示其同步性非常高，故通过房水中VEGF-A和VEGF-C的检测可推知角膜新生血管和淋巴管的生长情况。
[Abstract]:Objective: to count the corneal neovascularization (endothelial) and the corneal neovascularization (endothelial) in corneal tissue of corneal transplant patients with keratoplasty due to keratopathy, and to detect A(Vascular endothelial growth in aqueous humor by immunohistochemical staining. The expression of VEGF-A and C(Vascular endothelial growth factor-C- VEGF-C) was studied. To establish a quantitative relationship between VEGF-A and VEGF-C in aqueous humor and corneal neovascularization and corneal neovascularization lymphatic vessels, and to reveal the changes of VEGF-A and VEGF-C expression in aqueous humor and the number of corneal neovascularization and corneal neovascularization. To provide a theoretical basis for the choice of surgical timing for corneal transplantation in patients with keratopathy. Methods Ten patients who needed corneal transplantation due to various corneal ulcers and alkali burns were included in the study. The duration of the disease was recorded according to the history and the slit lamp photos of the patients before operation. To understand the preoperative status of the patients, the endothelial nonspecific receptor CD31 was used to quantitatively detect the number of corneal neovascularization and the number of blood vessels counting BVCs. The lymphatic endothelial cell-specific antibody (LYVE-1) was used for immunohistochemical staining. Quantitative detection of the number of lymphatic vessels in cornea (Lymphatic vessels counting). The aqueous humor of 10 experimental group samples and 10 control groups (simple age-related cataract patients) were tested by enzyme linked immunosorbent immunosorbent assay (Elisa) and compared with that of 10 control groups (simple age-related cataract patients) by enzyme-linked immunosorbent immunosorbent assay (Elisa). The expression of VEGF-A and VEGF-C in aqueous humor was measured and the relationship between the expression of VEGF-A and VEGF-C and BVC and LVC in aqueous humor was explored. Results: the expression of VEGF-A and VEGF-C in aqueous humor was significantly different between experimental group and control group, and the corneal neovascularization was found in 4 cases with neovascularization and lymphatic vessels, and the expression of VEGF-A and VEGF-C in aqueous humor of experimental group and control group were significantly different. There was a significant correlation between the expression of VEGF-A and VEGF-C in aqueous humor. Conclusion: the expression of VEGF-A and VEGF-C in aqueous humor of patients with corneal neovascularization and neovascularization after keratopathy was significantly increased. VEGF-A and corneal neovascularization were positively correlated with the number of corneal neovascularization. The trend analysis of the changes after normalization of corneal neovascularization showed that their synchronism was very high. Therefore, the growth of corneal neovascularization and lymphatic vessels could be inferred by the detection of VEGF-A and VEGF-C in aqueous humor.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R772.2

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