大鼠OSAS与缺血性脑卒中的关系研究

发布时间：2018-03-26 14:31

本文选题：阻塞性睡眠呼吸暂停综合征　切入点：C-反应蛋白　出处：《遵义医学院》2012年硕士论文

【摘要】：目的：通过建立大鼠阻塞性睡眠呼吸暂停综合征(obstructive sleep apnea syndrome, OSAS)模型及高血脂、高血糖因素参与,探讨阻塞性睡眠呼吸暂停综合征病理生理机制及其与缺血性脑卒中的相关性。方法：Wistar大鼠随机分为6组：正常对照组、高脂组、糖尿病组、阻塞性睡眠呼吸暂停综合征组、阻塞性睡眠呼吸暂停综合征+高脂组、阻塞性睡眠呼吸暂停综合征+糖尿病组。在大鼠咽腔多点注射透明质酸钠凝胶建立OSAS模型,监测(?)OSAS大鼠脑电图(electroencephalogram, EEG)、口鼻气流及动态血氧2小时,平均血氧饱和度、最低血氧饱和度及睡眠呼吸暂停指数造模前后统计有差异判定模型成功。①采用全自动生化分析仪测血清C-反应蛋白(C-reactive protein, CRP)水平,全自动血凝仪测血浆纤维蛋白原(fibrinogen, Fbg)水平。②ELISA法测血浆同型半胱氨酸(Homocysteine,Hcy)水平。③HE染色法观察大鼠皮质及海马结构。结果：①OSAS大鼠平均血氧饱和度(89.75±2.01)、最低血氧饱和度(72.77±5.19)降低,睡眠呼吸暂停指数(5.70±1.02)增高,与造模前比较有统计学差异(P0.01)。②OSAS组CRP(0.15±0.04)、Fbg(2.46±0.90)较正常对照组升高,与平均血氧饱和度成负相关(r分别为-0.802、-0.867,P0.01),与睡眠呼吸暂停指数成正相关(r分别为0.874、0.941,P0.05)；OSAS+高脂组大鼠CRP(0.15±0.04)、Fbg(2.78±1.50)较正常对照组升高,与平均血氧饱和度成负相关(r分别为-0.901、-0.963,P0.05),与睡眠呼吸暂停指数成正相关(r分别为0.933、0.975,P0.05)；OSAS+糖尿病组大鼠CRP(0.16±0.03)、Fbg(3.27±1.83)较正常对照组升高,与平均血氧饱和度成负相关(r分别为-0.881、-0.869,P0.05),与睡眠呼吸暂停指数成正相关(r分别为0.928、0.990,P0.05)。③OSAS+高脂组大鼠Hcy(10.94±2.48)水平较其它五组增高,差异有统计学意义(P0.05),与睡眠呼吸暂停指数成正相关(r为0.867,P0.05)。④OSAS组大鼠脑组织HE染色皮质及海马区有神经元缺失、排列紊乱及脑小血管增生,OSAS+高脂组、OSAS+糖尿病组较OSAS组改变明显。结论：①OSAS大鼠CRP、Fbg、Hcy水平升高,与OSAS病情程度密切相关。OSAS大鼠炎症反应增强、血液高凝状态、高同型半胱氨酸血症,可能是OSAS发生缺血性脑卒中的发病机制。②OSAS大鼠皮质及海马区有神经元缺失、排列紊乱及脑小血管增生表现。
[Abstract]:Objective: to investigate the pathophysiological mechanism of obstructive sleep apnea syndrome (OSASs) and its correlation with ischemic stroke by establishing a rat model of obstructive sleep apnea syndrome (OSASs) and the involvement of hyperlipidemia and hyperglycemia. Methods Wistar rats were randomly divided into 6 groups: normal control group, hyperlipidemia group, diabetes group, obstructive sleep apnea syndrome group, obstructive sleep apnea syndrome hyperlipidemia group. OSAS model was established by injecting sodium hyaluronate gel into the pharyngeal cavity of rats with obstructive sleep apnea syndrome diabetes mellitus. OSAS rats electroencephalograms, EEGG, oral and nasal airflow and dynamic oxygen for 2 hours, mean oxygen saturation, The statistical difference between the lowest oxygen saturation and sleep apnea index before and after the establishment of the model. 1 the serum level of C-reactive protein (CRPP) was measured by automatic biochemical analyzer. The plasma fibrinogen fibrinogen (Fbg) level was measured by automatic hemagglutination instrument. 2The plasma homocysteine homocysteine homocysteine (HcyH) level was measured by Elisa. 3 he staining was used to observe the structure of cortex and hippocampus in rats. Results the mean blood oxygen saturation and the lowest oxygen saturation were decreased, and the sleep apnea index was 5.70 卤1.02). Compared with the control group, there was significant difference between the two groups (P < 0.01). The mean oxygen saturation (CRP(0.15) in the control group was 2.46 卤0.90), which was higher than that in the normal control group (P < 0.01), and was significantly higher than that in the control group (P < 0.01), and was significantly higher than that in the normal control group (P < 0.05), and the sleep apnea index was 5.70 卤1.02 (P < 0.05). The negative correlation with the mean oxygen saturation was -0.802- 0.867U P0.01g, and the positive correlation with the sleep apnea index was 0.874U 0.941P0.05OSAS in the hyperlipidemic rats, CRP(0.15 卤0.04 FBG 2.78 卤1.50 was higher than that in the normal control group. The negative correlation with the mean oxygen saturation was -0.901U -0.963U P0.05A, and the positive correlation with the sleep apnea index was 0.933 卤0.975P0.05. the CRP(0.16 of the diabetic rats in the diabetic group was higher than that in the normal control group (3.27 卤1.83). The negative correlation with the mean oxygen saturation was -0.881- 0.869U P0.05A, and the positive correlation with the sleep apnea index was 0.928890g P0.05P0.05.3OSAS, the level of Hcy(10.94 卤2.48in the hyperlipidemia group was higher than that in the other five groups. The difference was statistically significant (P 0.05), and the positive correlation with the sleep apnea index was 0. 867 (P 0. 05) P 0. 05. 4OSAS. There were neuronal deletions in the cortex and hippocampus of the rats in the HE staining group. The changes of neurons in the hyperlipidemic group were significantly different from those in the OSAS group. Conclusion the level of FbgCy in CRP1OSAS rats is increased, which is closely related to the severity of OSAS. The inflammatory response is increased, blood hypercoagulability and hyperhomocysteinemia are observed in OSAS rats. It may be the pathogenesis of ischemic stroke in OSAS. There are neuronal deletion, disarrangement and small vascular hyperplasia in cortex and hippocampus of OSAS rats.
【学位授予单位】：遵义医学院
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R766;R743.3

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